APTT, Activated Partial Thromboplastin Time
Order Code:
PTT
Test Summary
Activated Partial Thromboplastin Time (aPTT, APTT, PTT) is a common screening assay to detect inherited or acquired factor deficiencies, inhibitors and monitoring the effectiveness of standard heparin therapy. All inherited and acquired deficiencies, inhibitors and heparin cause prolonged results. The ability of the APTT reagent to detect these disorders is related to the sensitivity of the reagent selected for use in this assay.
Aliases
- Activated PTT APTT PTT
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tube
Minimum Volume
1 Frozen Capped aliquot of Sodium Citrate plasma
Instructions
Use standard venipuncture and disposal techniques.
Collect the required quantity of Sodium Citrate (light blue top) tubes.
If unable to process specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.
Sodium Citrate (light blue top) tube(s): Process immediately (stable only 4 hours), following instructions below:
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat. Note: If the plasma shows any hemolysis, it must be recollected.
3. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
4. Finally, place all aliquots into a <-20°C freezer within 4 hours. Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.
Collect the required quantity of Sodium Citrate (light blue top) tubes.
If unable to process specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.
Sodium Citrate (light blue top) tube(s): Process immediately (stable only 4 hours), following instructions below:
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat. Note: If the plasma shows any hemolysis, it must be recollected.
3. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
4. Finally, place all aliquots into a <-20°C freezer within 4 hours. Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.
Patient Preparation
N/A
Storage
Whole blood Sodium Citrate (light blue top) tube
OR
Frozen - Capped aliquots of Sodium Citrate plasma
OR
Frozen - Capped aliquots of Sodium Citrate plasma
Transport Temperature
Room Temperature: Whole blood Sodium Citrate (light blue top) tube(s)
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma
Specimen Stability
- Room Temperature: Whole blood Sodium Citrate (light blue top) tube <
- 4 hours
- Refrigerated: Unacceptable for Sodium Citrate (light blue top) tube whole blood or capped plasma aliquots
- Frozen: Capped aliquots of Sodium Citrate plasma <
- 14 days at <
- &minus
- 20 degrees C or <
- 6 months at <
- &minus
- 70 degrees C
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Rejected for hemolysis, clotted, improper fill, incorrect specimen type, thawed frozen aliquots, refrigerated plasma or blood, expired test tubes, >4 hours old if not processed into separated frozen plasma vials.
Order Code
PTT
EPIC (Premier) Code
LAB012
Includes
N/A
CPT Code
- 85730
Billing Code
- 300492
CPT Statement
Methodology
Mechanical Clot Detection
FDA Status
FDA approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Department
Hematology
Reference Range
24.5-35.2 seconds
Setup Schedule / Expected Turnaround Time
Daily; Report available: 1 day
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tube
Minimum Volume
1 Frozen Capped aliquot of Sodium Citrate plasma
Instructions
Use standard venipuncture and disposal techniques.
Collect the required quantity of Sodium Citrate (light blue top) tubes.
If unable to process specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.
Sodium Citrate (light blue top) tube(s): Process immediately (stable only 4 hours), following instructions below:
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat. Note: If the plasma shows any hemolysis, it must be recollected.
3. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
4. Finally, place all aliquots into a <-20°C freezer within 4 hours. Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.
Collect the required quantity of Sodium Citrate (light blue top) tubes.
If unable to process specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.
Sodium Citrate (light blue top) tube(s): Process immediately (stable only 4 hours), following instructions below:
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat. Note: If the plasma shows any hemolysis, it must be recollected.
3. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
4. Finally, place all aliquots into a <-20°C freezer within 4 hours. Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.
Patient Preparation
N/A
Storage
Whole blood Sodium Citrate (light blue top) tube
OR
Frozen - Capped aliquots of Sodium Citrate plasma
OR
Frozen - Capped aliquots of Sodium Citrate plasma
Transport Temperature
Room Temperature: Whole blood Sodium Citrate (light blue top) tube(s)
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma
Specimen Stability
- Room Temperature: Whole blood Sodium Citrate (light blue top) tube <
- 4 hours
- Refrigerated: Unacceptable for Sodium Citrate (light blue top) tube whole blood or capped plasma aliquots
- Frozen: Capped aliquots of Sodium Citrate plasma <
- 14 days at <
- &minus
- 20 degrees C or <
- 6 months at <
- &minus
- 70 degrees C
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Rejected for hemolysis, clotted, improper fill, incorrect specimen type, thawed frozen aliquots, refrigerated plasma or blood, expired test tubes, >4 hours old if not processed into separated frozen plasma vials.
Result Information
Methodology
Mechanical Clot Detection
Testing Laboratory
N/A
Reference Range
24.5-35.2 seconds
Setup Schedule / Expected Turnaround Time
Daily; Report available: 1 day