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PT/APTT

Test Summary

PT or Prothrombin Time Screening test useful in those patients with high risk to predict post op hemorrhage. The Prothrombin Time (PT) in conjunction with the INR is used to monitor oral anticoagulants such as coumadin. Additional uses for PT testing in patients where acquired multiple deficiencies may be suspected. Such disorders may include DIC, liver disease, renal disease and severe malabsorption. Activated Partial Thromboplastin Time (aPTT) is a common screening assay to detect inherited or acquired factor deficiencies or the presence of inhibitors.

Aliases

  • N/A

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tube

Minimum Volume

1 Frozen Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)

Instructions

Use standard venipuncture and disposal techniques.

Collect the required quantity of Sodium Citrate (light blue top) tubes.

If unable to process specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.
Sodium Citrate (light blue top) tube(s): Process immediately (stable only 4 hours), following instructions below:
Note: We recommend aliquot tubes to be made of polypropylene plastic as this material is safe to freeze at <-70 degrees C.
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat.
Note: If the plasma shows any hemolysis, it must be recollected.
3. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
4. Finally, place all aliquots into a <-20°C freezer within 4 hours.
Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.

Patient Preparation

N/A

Storage

Whole blood Sodium Citrate (light blue top) tube(s)
OR
Frozen - Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)

Transport Temperature

Room Temperature: Whole blood Sodium Citrate (light blue top) tube(s)
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma

Specimen Stability

  • Room Temperature: Whole blood Sodium Citrate (light blue top) tube <
  • 4 hours
  • Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
  • Frozen: Capped aliquots of Sodium Citrate plasma <
  • 14 days at <
  • &minus
  • 20 degrees C
  • or <
  • 6 months at <
  • &minus
  • 70 degrees C

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

Rejected for hemolysis, clotted, improper fill, incorrect specimen type, thawed frozen aliquots, refrigerated plasma or blood, expired test tubes, >4 hours old if not processed into separated frozen plasma vials.

Order Code

PTPTT

EPIC (Premier) Code

LAB700

Includes

Prothrombin Time w/INR, Partial Thromboplastin Time Activated

CPT Code

  • 85610
  • 85730

Billing Code

  • 300482
  • 300492

CPT Statement

Methodology

Mechanical Clot Detection

FDA Status

FDA approved

Physician Attestation of Informed Consent

N/A

Testing Laboratory

CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439

Department

Hematology

Reference Range

  • PT=11.7-13.9 Seconds and
  • APTT=24.5-35.2 Seconds

Setup Schedule / Expected Turnaround Time

24/7; Report available: 1 day

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tube

Minimum Volume

1 Frozen Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)

Instructions

Use standard venipuncture and disposal techniques.

Collect the required quantity of Sodium Citrate (light blue top) tubes.

If unable to process specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.
Sodium Citrate (light blue top) tube(s): Process immediately (stable only 4 hours), following instructions below:
Note: We recommend aliquot tubes to be made of polypropylene plastic as this material is safe to freeze at <-70 degrees C.
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat.
Note: If the plasma shows any hemolysis, it must be recollected.
3. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
4. Finally, place all aliquots into a <-20°C freezer within 4 hours.
Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.

Patient Preparation

N/A

Storage

Whole blood Sodium Citrate (light blue top) tube(s)
OR
Frozen - Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)

Transport Temperature

Room Temperature: Whole blood Sodium Citrate (light blue top) tube(s)
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma

Specimen Stability

  • Room Temperature: Whole blood Sodium Citrate (light blue top) tube <
  • 4 hours
  • Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
  • Frozen: Capped aliquots of Sodium Citrate plasma <
  • 14 days at <
  • &minus
  • 20 degrees C
  • or <
  • 6 months at <
  • &minus
  • 70 degrees C

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

Rejected for hemolysis, clotted, improper fill, incorrect specimen type, thawed frozen aliquots, refrigerated plasma or blood, expired test tubes, >4 hours old if not processed into separated frozen plasma vials.

Billing

CPT Code

  • 85610
  • 85730

Billing Code

  • 300482
  • 300492

CPT Statement

Result Information

Methodology

Mechanical Clot Detection

Testing Laboratory

N/A

Reference Range

  • PT=11.7-13.9 Seconds and
  • APTT=24.5-35.2 Seconds

Setup Schedule / Expected Turnaround Time

24/7; Report available: 1 day