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Paroxysmal Nocturnal Hemoglobinuria (PNH) with FLAER (High Sensitivity)

Test Summary

The high sensitivity and quantitative flow cytometry assay is used in the diagnosis and follow-up of patients with paroxysmal nocturnal hemoglobinuria (PNH). FLAER as well as antibodies directed against glycosylphosphosphatidylinositol (GPI)-linked antigens are used to evaluate for the presence of (GPI)-deficient cell populations down to a level of 0.01%.

Aliases

  • N/A

Specimen Collection

Special Instructions

N/A

Preferred Specimen

5-10 mL whole blood collected in a sodium heparin (green-top) tube

Minimum Volume

3 mL

Instructions

N/A

Patient Preparation

N/A

Storage

N/A

Transport Temperature

Room temperature

Specimen Stability

  • Room temperature: 72 hours
  • Refrigerated: Unacceptable
  • Frozen: Unacceptable

Limitations

N/A

Other Acceptable Specimens

Whole blood collected in: ACD-A (yellow-top) tube or EDTA (lavender-top) tube

Unacceptable Specimens

N/A

Order Code

PNHFL

EPIC (Premier) Code

LAB6384

Includes

Antibodies directed against CD45, glycophorin A, CD59, CD24, CD14, CD15, CD64, as well as FLAER.

CPT Code

  • 88184
  • 88185 (x7)
  • 88187 - This test code is for non-New York patient testing. For New York patient testing

Billing Code

  • 670786
  • 670787
  • 670788

CPT Statement

Methodology

Flow Cytometry (FC)

FDA Status

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153

Department

Reference Testing

Reference Range

See Laboratory Report

Setup Schedule / Expected Turnaround Time

Daily; Report available: 2 - 3 days

Specimen Collection

Special Instructions

N/A

Preferred Specimen

5-10 mL whole blood collected in a sodium heparin (green-top) tube

Minimum Volume

3 mL

Instructions

N/A

Patient Preparation

N/A

Storage

N/A

Transport Temperature

Room temperature

Specimen Stability

  • Room temperature: 72 hours
  • Refrigerated: Unacceptable
  • Frozen: Unacceptable

Limitations

N/A

Other Acceptable Specimens

Whole blood collected in: ACD-A (yellow-top) tube or EDTA (lavender-top) tube

Unacceptable Specimens

N/A

Billing

CPT Code

  • 88184
  • 88185 (x7)
  • 88187 - This test code is for non-New York patient testing. For New York patient testing

Billing Code

  • 670786
  • 670787
  • 670788

CPT Statement

Result Information

Methodology

Flow Cytometry (FC)

Testing Laboratory

N/A

Reference Range

See Laboratory Report

Setup Schedule / Expected Turnaround Time

Daily; Report available: 2 - 3 days