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Penicilloyl G (C1) IgE

Test Summary

This procedure is based on a fluorescent enzyme immunoassay (FEIA) technique. The allergen of interest, covalently coupled to an ImmunoCap, reacts with the specific IgE in the patient's serum. After washing away nonspecific IgE, enzyme-labelled antibodies against IgE are added to form a complex. After incubation, unbound enzyme anti-IgE is washed away and the bound complex is then incubated with a developing agent. After the reaction is stopped, the fluorescence of the eluate is measured.
The higher the fluorescence value, the more specific IgE is present in the specimen. To classify test results, fluorescence for patient samples is compared directly with fluorescence for a reference serum run in parallel. Results are reported in both kU/L and Conventional Class score.

Aliases

  • N/A

Specimen Collection

Special Instructions

N/A

Preferred Specimen

0.3 mL serum

Minimum Volume

0.15 mL

Instructions

Collect blood in a red-top (no gel) or serum separator tube (red-top). Allow blood to clot (10-15 minutes) at room temperature. Centrifuge and separate the serum from the cells and transfer serum to a clean transport tube.

Patient Preparation

N/A

Storage

Transport tube

Transport Temperature

Room temperature

Specimen Stability

  • Room temperature: 14 days
  • Refrigerated: 14 days
  • Frozen: 30 days

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

N/A

Order Code

PENGC1

EPIC (Premier) Code

LAB3472

Includes

N/A

CPT Code

  • 86003

Billing Code

  • 660029

CPT Statement

Methodology

Immunoassay (IA)

FDA Status

FDA Approved

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153

Department

Reference Testing

Reference Range

See Laboratory Report

Setup Schedule / Expected Turnaround Time

Monday - Saturday; Report available: 1-3 days

Specimen Collection

Special Instructions

N/A

Preferred Specimen

0.3 mL serum

Minimum Volume

0.15 mL

Instructions

Collect blood in a red-top (no gel) or serum separator tube (red-top). Allow blood to clot (10-15 minutes) at room temperature. Centrifuge and separate the serum from the cells and transfer serum to a clean transport tube.

Patient Preparation

N/A

Storage

Transport tube

Transport Temperature

Room temperature

Specimen Stability

  • Room temperature: 14 days
  • Refrigerated: 14 days
  • Frozen: 30 days

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

N/A

Billing

CPT Code

  • 86003

Billing Code

  • 660029

CPT Statement

Result Information

Methodology

Immunoassay (IA)

Testing Laboratory

N/A

Reference Range

See Laboratory Report

Setup Schedule / Expected Turnaround Time

Monday - Saturday; Report available: 1-3 days