Mycophenolic Acid, LC/MS/MS
Test Summary
Mycophenolic acid is an immunosuppressant used in tissue transplants. It prevents graft rejection by the host's immune system. It is very important to monitor its level. Too little of this drug will cause graft rejectoin, while too much will lead to infection. Monitoring its level is essential to optimize therapeutic effects, avoid toxicity, and assure compliance.
Aliases
- CELLECPT
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL serum collected in a red-top tube (no gel)
Minimum Volume
0.5 mL
Instructions
Serum (preferred): Collect blood in a plain red-top tube (no gel). Allow blood to clot at 15-28° C for 20-30 minutes. Centrifuge at 25-28° C (2200-2500 rpm, 1000 x g) for 8-10 minutes. Transfer serum to polypropylene or polyethylene transport tube. Ship refrigerated (cold packs). Samples left at room temperature for >72 hours are unacceptable.
Plasma (acceptable): Draw blood into an EDTA (lavender-top) tube. Separate cells by centrifuge at 18-25° C (2500-2800 rpm) for 5-10 minutes. Transfer plasma to polypropylene or polyethylene transport tube. Ship refrigerated (cold packs). Samples left at room temperature for >72 hours are unacceptable.
Plasma (acceptable): Draw blood into an EDTA (lavender-top) tube. Separate cells by centrifuge at 18-25° C (2500-2800 rpm) for 5-10 minutes. Transfer plasma to polypropylene or polyethylene transport tube. Ship refrigerated (cold packs). Samples left at room temperature for >72 hours are unacceptable.
Patient Preparation
Optimum time to collect sample: 1/2 hour to 1 hour before next dose (trough) at steady-state (3-5 days after treatment with oral doses)
Storage
Plastic screw-cap vial
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
- Room temperature: 72 hours
- Refrigerated: 14 days
- Frozen: 60 days
Limitations
N/A
Other Acceptable Specimens
Plasma collected in: Sodium heparin (green-top) tube or EDTA (lavender-top) tube
Unacceptable Specimens
Hemolysis • Lipemia • Improperly labeled specimens • Specimens left at room temperature >72 hours • Gel barrier/serum separator tube (SST)
Order Code
MYCPHN
EPIC (Premier) Code
LAB3066
Includes
Mycophenolic Acid and MPA Glucuronide
CPT Code
- 80180
Billing Code
- 671024
CPT Statement
Methodology
Chromatography/Mass Spectrometry
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153
14225 Newbrook Drive
Chantilly VA, 20153
Department
Reference Testing
Reference Range
| Mycophenolic Acid | 1.0-3.5 mcg/mL |
| MPA Glucuronide | 35.0-100.0 mcg/mL |
Setup Schedule / Expected Turnaround Time
Monday - Saturday morning; Report available: 2 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL serum collected in a red-top tube (no gel)
Minimum Volume
0.5 mL
Instructions
Serum (preferred): Collect blood in a plain red-top tube (no gel). Allow blood to clot at 15-28° C for 20-30 minutes. Centrifuge at 25-28° C (2200-2500 rpm, 1000 x g) for 8-10 minutes. Transfer serum to polypropylene or polyethylene transport tube. Ship refrigerated (cold packs). Samples left at room temperature for >72 hours are unacceptable.
Plasma (acceptable): Draw blood into an EDTA (lavender-top) tube. Separate cells by centrifuge at 18-25° C (2500-2800 rpm) for 5-10 minutes. Transfer plasma to polypropylene or polyethylene transport tube. Ship refrigerated (cold packs). Samples left at room temperature for >72 hours are unacceptable.
Plasma (acceptable): Draw blood into an EDTA (lavender-top) tube. Separate cells by centrifuge at 18-25° C (2500-2800 rpm) for 5-10 minutes. Transfer plasma to polypropylene or polyethylene transport tube. Ship refrigerated (cold packs). Samples left at room temperature for >72 hours are unacceptable.
Patient Preparation
Optimum time to collect sample: 1/2 hour to 1 hour before next dose (trough) at steady-state (3-5 days after treatment with oral doses)
Storage
Plastic screw-cap vial
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
- Room temperature: 72 hours
- Refrigerated: 14 days
- Frozen: 60 days
Limitations
N/A
Other Acceptable Specimens
Plasma collected in: Sodium heparin (green-top) tube or EDTA (lavender-top) tube
Unacceptable Specimens
Hemolysis • Lipemia • Improperly labeled specimens • Specimens left at room temperature >72 hours • Gel barrier/serum separator tube (SST)
Result Information
Methodology
Chromatography/Mass Spectrometry
Testing Laboratory
N/A
Reference Range
| Mycophenolic Acid | 1.0-3.5 mcg/mL |
| MPA Glucuronide | 35.0-100.0 mcg/mL |
Setup Schedule / Expected Turnaround Time
Monday - Saturday morning; Report available: 2 days