MPL Mutation Analysis
Test Summary
This DNA-based assay tests leukocytes from blood or bone marrow aspirate for mutations in exon 10 of the thrombopoietin receptor (MPL), including codons 505 and 515, using an advanced DNA sequencing method. Mutations at these sites in MPL are associated with myeloproliferative neoplasms, particularly essential thrombocythemia and primary myelofibrosis.
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
4 mL whole blood or 3 mL bone marrow aspirate collected in an EDTA (lavender-top) tube
Minimum Volume
3 mL whole blood • 1 mL bone marrow aspirate
Instructions
Note: Do not reject specimens, send to lab for screening.
Cell Pellets: Send fixed samples within 7 days of fixation date in a plastic, leak-proof tube/container. Indicate type of fixative used along with patient name, specimen source, date collected, and date harvested/fixed. Cell pellets submitted in Carnoy's or other alcohol-based fixatives are acceptable.
Cell Pellets: Send fixed samples within 7 days of fixation date in a plastic, leak-proof tube/container. Indicate type of fixative used along with patient name, specimen source, date collected, and date harvested/fixed. Cell pellets submitted in Carnoy's or other alcohol-based fixatives are acceptable.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Whole blood, Bone marrow aspirate, Fixed cell pellets
- Room temperature: 7 days
- Refrigerated: 7 days
- Frozen: Unacceptable
- Extracted DNA
- Room temperature:7 days
- Refrigerated: 1
Limitations
N/A
Other Acceptable Specimens
Whole blood or bone marrow aspirate collected in a sodium heparin (green-top) tube • Fixed Cell pellet collected in a plastic leak-proof container • Extracted DNA from CLIA-certified laboratory collected in a sterile leak-proof container
Unacceptable Specimens
N/A
Order Code
MPLMUT
EPIC (Premier) Code
LAB6124
Includes
N/A
CPT Code
- 81339
Billing Code
- 670857
CPT Statement
Methodology
Polymerase Chain Reaction-based DNA Sequencing
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153
14225 Newbrook Drive
Chantilly VA, 20153
Department
Reference Testing
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Daily; Report available: 5 - 7 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
4 mL whole blood or 3 mL bone marrow aspirate collected in an EDTA (lavender-top) tube
Minimum Volume
3 mL whole blood • 1 mL bone marrow aspirate
Instructions
Note: Do not reject specimens, send to lab for screening.
Cell Pellets: Send fixed samples within 7 days of fixation date in a plastic, leak-proof tube/container. Indicate type of fixative used along with patient name, specimen source, date collected, and date harvested/fixed. Cell pellets submitted in Carnoy's or other alcohol-based fixatives are acceptable.
Cell Pellets: Send fixed samples within 7 days of fixation date in a plastic, leak-proof tube/container. Indicate type of fixative used along with patient name, specimen source, date collected, and date harvested/fixed. Cell pellets submitted in Carnoy's or other alcohol-based fixatives are acceptable.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Whole blood, Bone marrow aspirate, Fixed cell pellets
- Room temperature: 7 days
- Refrigerated: 7 days
- Frozen: Unacceptable
- Extracted DNA
- Room temperature:7 days
- Refrigerated: 1
Limitations
N/A
Other Acceptable Specimens
Whole blood or bone marrow aspirate collected in a sodium heparin (green-top) tube • Fixed Cell pellet collected in a plastic leak-proof container • Extracted DNA from CLIA-certified laboratory collected in a sterile leak-proof container
Unacceptable Specimens
N/A
Result Information
Methodology
Polymerase Chain Reaction-based DNA Sequencing
Testing Laboratory
N/A
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Daily; Report available: 5 - 7 days