FISH, MET Amplification
Order Code:
METFSH
Test Summary
The proto-oncogene MET (c-MET) product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. Overexpression of MET has been observed in variety of neoplasms such as kidney, lung, head and neck, ovary, breast, thyroid, brain, stomach, pancreas and colon. MET gene amplification, detected by FISH, has been associated with an unfavorable prognosis in non-small cell lung cancer (NSCLC) and resistance to EGFR inhibitors.
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
Formalin-fixed, paraffin-embedded tissue block
Minimum Volume
N/A
Instructions
For MET determination in solid tumors, only biopsies with histologically proven tumor involvement should be tested.
Samples that can be tested include tumor biopsy in tissue culture media, formalin fixed paraffin-embedded block or a minimum of 4 charged/ (+)slides from formalin fixed paraffin embedded tissue. Specimen MUST be fixed in 10% neutral buffered formalin. Fixation between 6 and 48 hours is recommended.
Pathology report must accompany paraffin block or slides.
Specimen viability decreases in transit. Send specimen to testing lab for viability determination. Do not reject.
Note: 100 interphase cells are microscopically scored for the copy number of MET and centromere 7 to detect amplification.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Samples that can be tested include tumor biopsy in tissue culture media, formalin fixed paraffin-embedded block or a minimum of 4 charged/ (+)slides from formalin fixed paraffin embedded tissue. Specimen MUST be fixed in 10% neutral buffered formalin. Fixation between 6 and 48 hours is recommended.
Pathology report must accompany paraffin block or slides.
Specimen viability decreases in transit. Send specimen to testing lab for viability determination. Do not reject.
Note: 100 interphase cells are microscopically scored for the copy number of MET and centromere 7 to detect amplification.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: See instructions
- Refrigerated: See instructions
- Frozen: See instructions
Limitations
N/A
Other Acceptable Specimens
5 x5mm tumor tissue collected in culture transport medium, or 5 micron (4 micron minimum) sections collected on each of (x5) charged slides
Unacceptable Specimens
N/A
Order Code
METFSH
EPIC (Premier) Code
LAB5315
Includes
N/A
CPT Code
- 88271 (x2)
- 88275
Billing Code
- 671080
- 671081
CPT Statement
Methodology
Fluorescence In Situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153
14225 Newbrook Drive
Chantilly VA, 20153
Department
Reference Testing
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Daily; Report available: 5 - 7 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
Formalin-fixed, paraffin-embedded tissue block
Minimum Volume
N/A
Instructions
For MET determination in solid tumors, only biopsies with histologically proven tumor involvement should be tested.
Samples that can be tested include tumor biopsy in tissue culture media, formalin fixed paraffin-embedded block or a minimum of 4 charged/ (+)slides from formalin fixed paraffin embedded tissue. Specimen MUST be fixed in 10% neutral buffered formalin. Fixation between 6 and 48 hours is recommended.
Pathology report must accompany paraffin block or slides.
Specimen viability decreases in transit. Send specimen to testing lab for viability determination. Do not reject.
Note: 100 interphase cells are microscopically scored for the copy number of MET and centromere 7 to detect amplification.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Samples that can be tested include tumor biopsy in tissue culture media, formalin fixed paraffin-embedded block or a minimum of 4 charged/ (+)slides from formalin fixed paraffin embedded tissue. Specimen MUST be fixed in 10% neutral buffered formalin. Fixation between 6 and 48 hours is recommended.
Pathology report must accompany paraffin block or slides.
Specimen viability decreases in transit. Send specimen to testing lab for viability determination. Do not reject.
Note: 100 interphase cells are microscopically scored for the copy number of MET and centromere 7 to detect amplification.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: See instructions
- Refrigerated: See instructions
- Frozen: See instructions
Limitations
N/A
Other Acceptable Specimens
5 x5mm tumor tissue collected in culture transport medium, or 5 micron (4 micron minimum) sections collected on each of (x5) charged slides
Unacceptable Specimens
N/A
Result Information
Methodology
Fluorescence In Situ Hybridization (FISH)
Testing Laboratory
N/A
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Daily; Report available: 5 - 7 days