FISH, MALT Lymphoma, MALT1, rea18q21 with Reflex to API2/MALT1, t(11;18)
Test Summary
The extra-nodal marginal zone B-cell lymphoma (MZBCL) of mucosa associated lymphoid tissue (MALT) type, which comprises 7% of all non-Hodgkin lymphomas, is an indolent disease, involving most often the stomach, where it usually follows chronic gastritis, due to Helicobacter pylori (HP) infection. This lymphoma may also localize in the lung, the thyroid, the salivary gland, and the orbit. Two common translocations in this lymphoma are the t(11;18)(q12;q21) (20-50% of cases) and the t(14;18) (q32;q21) (10-20% of cases). This break apart 18q21 probe is able to detect either of these translocations. It is suggested to follow up a positive FISH result with the specific dual-fusion API2/MALT1 probe to detect the presence of the translocation (11;18) since this is often associated with resistance to HP treatment.
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
5 mL whole blood collected in a sodium heparin (green-top) tube, or
3 mL bone marrow collected in a sodium heparin (green-top) tube
3 mL bone marrow collected in a sodium heparin (green-top) tube
Minimum Volume
1 mL
Instructions
Clinical history and reason for referral are required with test order. Prior therapy and transplant history should be provided with test order.
For bone marrow (1-3 mL) or whole blood (3-5 mL) use green top (sodium heparin) tube. For lymph node biopsy 5x5 mm in a sterile container in Hank's Ringer's solution or culture medium with antibiotics. Transportation medium available upon request. Formalin-fixed, paraffin-embedded tissue.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
For bone marrow (1-3 mL) or whole blood (3-5 mL) use green top (sodium heparin) tube. For lymph node biopsy 5x5 mm in a sterile container in Hank's Ringer's solution or culture medium with antibiotics. Transportation medium available upon request. Formalin-fixed, paraffin-embedded tissue.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: See instructions
- Refrigerated: See instructions
- Frozen: See instructions
Limitations
N/A
Other Acceptable Specimens
Formalin-fixed, paraffin-embedded tissue • 5x5 mm lymph node tissue or lymph node collected in a sterile container in Hank's Ringer's solution or culture medium with antibiotics
Unacceptable Specimens
N/A
Order Code
MALT1
EPIC (Premier) Code
N/A
Includes
If FISH, MALT Lymphoma, MALT1, rea18q21 is positive, then FISH, MALT Lymphoma, API2/MALT1, t(11;18) will be performed at an additional charge (CPT code(s): 88271 (x2), 88275),
CPT Code
- 88271 (x2)
- 88275
Billing Code
- 671072
- 671073
CPT Statement
Methodology
Fluorescence In Situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153
14225 Newbrook Drive
Chantilly VA, 20153
Department
Reference Testing
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Sunday - Friday; Report available: 7 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
5 mL whole blood collected in a sodium heparin (green-top) tube, or
3 mL bone marrow collected in a sodium heparin (green-top) tube
3 mL bone marrow collected in a sodium heparin (green-top) tube
Minimum Volume
1 mL
Instructions
Clinical history and reason for referral are required with test order. Prior therapy and transplant history should be provided with test order.
For bone marrow (1-3 mL) or whole blood (3-5 mL) use green top (sodium heparin) tube. For lymph node biopsy 5x5 mm in a sterile container in Hank's Ringer's solution or culture medium with antibiotics. Transportation medium available upon request. Formalin-fixed, paraffin-embedded tissue.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
For bone marrow (1-3 mL) or whole blood (3-5 mL) use green top (sodium heparin) tube. For lymph node biopsy 5x5 mm in a sterile container in Hank's Ringer's solution or culture medium with antibiotics. Transportation medium available upon request. Formalin-fixed, paraffin-embedded tissue.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: See instructions
- Refrigerated: See instructions
- Frozen: See instructions
Limitations
N/A
Other Acceptable Specimens
Formalin-fixed, paraffin-embedded tissue • 5x5 mm lymph node tissue or lymph node collected in a sterile container in Hank's Ringer's solution or culture medium with antibiotics
Unacceptable Specimens
N/A
Result Information
Methodology
Fluorescence In Situ Hybridization (FISH)
Testing Laboratory
N/A
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Sunday - Friday; Report available: 7 days