Legionella Pneumophila Antigen, DFA
Test Summary
Bacteria in the genus Legionella primarily cause respiratory illness, i.e., either Legionnaires' Disease, a systemic illness manifested primarily by pneumonia, or Pontiac Fever, a non-pneumonic, influenza-like illness. Legionellae may also cause a variety of other illnesses.
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 gram fresh (unfixed)lung or other respiratory tissue submitted in a sterile leak proof container or 3 mL bronchial or transtracheal wash/lavage/aspirate or sputum or pleural fluid submitted in a sterile leak-proof container
Minimum Volume
1 g lung tissue or other respiratory tissue • 2 mL respiratory fluids
Instructions
Lung Tissue: Place into sterile container with a small amount of sterile, non-bacteriostatic, distilled water. Do not add saline because it may be inhibitory.
Sputum: Instruct patient to gargle with water and cough deeply to collect deep respiratory specimen in sterile container.
Sputum: Instruct patient to gargle with water and cough deeply to collect deep respiratory specimen in sterile container.
Patient Preparation
N/A
Storage
Sterile leak-proof container
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
- Room temperature: Unacceptable
- Refrigerated: 7 days
- Frozen: Unacceptable
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Slides: serum; urine; expired transport device; environmental sources; specimens received in a viral transport media
Order Code
LEGNAG
EPIC (Premier) Code
LAB546
Includes
N/A
CPT Code
- 87278
Billing Code
- 700000
CPT Statement
Methodology
Direct Immunofluorescence Assay (DFA)
FDA Status
FDA Approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153
14225 Newbrook Drive
Chantilly VA, 20153
Reference Range
Not detected
Setup Schedule / Expected Turnaround Time
Monday - Saturday; Report available: 1 day
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 gram fresh (unfixed)lung or other respiratory tissue submitted in a sterile leak proof container or 3 mL bronchial or transtracheal wash/lavage/aspirate or sputum or pleural fluid submitted in a sterile leak-proof container
Minimum Volume
1 g lung tissue or other respiratory tissue • 2 mL respiratory fluids
Instructions
Lung Tissue: Place into sterile container with a small amount of sterile, non-bacteriostatic, distilled water. Do not add saline because it may be inhibitory.
Sputum: Instruct patient to gargle with water and cough deeply to collect deep respiratory specimen in sterile container.
Sputum: Instruct patient to gargle with water and cough deeply to collect deep respiratory specimen in sterile container.
Patient Preparation
N/A
Storage
Sterile leak-proof container
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
- Room temperature: Unacceptable
- Refrigerated: 7 days
- Frozen: Unacceptable
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Slides: serum; urine; expired transport device; environmental sources; specimens received in a viral transport media
Result Information
Methodology
Direct Immunofluorescence Assay (DFA)
Testing Laboratory
N/A
Reference Range
Not detected
Setup Schedule / Expected Turnaround Time
Monday - Saturday; Report available: 1 day