JAK2 V617F Mutation Analysis
Order Code:
JK2617
Test Summary
This advanced DNA-based, next-generation sequencing (NGS) assay analyses leukocytes from blood or bone marrow aspirate for mutation at condon 617 of JAK2. The JAK2 V617F mutation is associated with myeloproliferative neoplasms, including polycythemia vera, essential thrombocythemia and primary myelofibrosis.
Aliases
- Myeloproliferative neoplasm
- PNF
- MPN
- Next-Generation Sequencing Essential Thrombocythemia (ET)
- NGS
- Janus Kinase 2
- Polycythemia Vera (PV)
- JAK
- ET
- Primary Myelofibrosis (PMF)
- PV
Specimen Collection
Special Instructions
N/A
Preferred Specimen
4 mL whole blood or 3 mL bone marrow aspirate collected in an EDTA (lavender-top) tube
Minimum Volume
3 mL whole blood • 1.0 mL bone marrow aspirate
Instructions
Do not reject specimens, send to laboratory for screening.
Cell Pellets: Send fixed samples within 7 days of fixation date in a plastic, leak-proof tube/container. Indicate type of fixative used along with patient name, specimen source, date collected, and date harvested/fixed. Cell pellets submitted in Carnoy's or other alcohol-based fixatives are acceptable.
Cell Pellets: Send fixed samples within 7 days of fixation date in a plastic, leak-proof tube/container. Indicate type of fixative used along with patient name, specimen source, date collected, and date harvested/fixed. Cell pellets submitted in Carnoy's or other alcohol-based fixatives are acceptable.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Whole blood, Bone marrow aspirate, Fixed cell pellets:
- Room temperature: 7 days
- Refrigerated: 7 days
- Frozen: Unacceptable
- Extracted DNA:
- Room temperature:7 days
- Refrigerated: 14 days
- Frozen: 1 day
Limitations
N/A
Other Acceptable Specimens
Whole blood or bone marrow aspirate collected in: Sodium heparin (green-top) tube• Fixed cell pellets collected in a plastic leak-proof container• Extracted DNA from CLIA-certified laboratory collected in a sterile lead-proof container
Unacceptable Specimens
Do not reject specimens, send to laboratory for screening
Order Code
JK2617
EPIC (Premier) Code
LAB6122
Includes
N/A
CPT Code
- 81270
Billing Code
- 671151
CPT Statement
Methodology
Next-Generation Sequencing
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153
14225 Newbrook Drive
Chantilly VA, 20153
Department
Reference Testing
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Daily; Report available: 5 - 7 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
4 mL whole blood or 3 mL bone marrow aspirate collected in an EDTA (lavender-top) tube
Minimum Volume
3 mL whole blood • 1.0 mL bone marrow aspirate
Instructions
Do not reject specimens, send to laboratory for screening.
Cell Pellets: Send fixed samples within 7 days of fixation date in a plastic, leak-proof tube/container. Indicate type of fixative used along with patient name, specimen source, date collected, and date harvested/fixed. Cell pellets submitted in Carnoy's or other alcohol-based fixatives are acceptable.
Cell Pellets: Send fixed samples within 7 days of fixation date in a plastic, leak-proof tube/container. Indicate type of fixative used along with patient name, specimen source, date collected, and date harvested/fixed. Cell pellets submitted in Carnoy's or other alcohol-based fixatives are acceptable.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Whole blood, Bone marrow aspirate, Fixed cell pellets:
- Room temperature: 7 days
- Refrigerated: 7 days
- Frozen: Unacceptable
- Extracted DNA:
- Room temperature:7 days
- Refrigerated: 14 days
- Frozen: 1 day
Limitations
N/A
Other Acceptable Specimens
Whole blood or bone marrow aspirate collected in: Sodium heparin (green-top) tube• Fixed cell pellets collected in a plastic leak-proof container• Extracted DNA from CLIA-certified laboratory collected in a sterile lead-proof container
Unacceptable Specimens
Do not reject specimens, send to laboratory for screening
Result Information
Methodology
Next-Generation Sequencing
Testing Laboratory
N/A
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Daily; Report available: 5 - 7 days