HLA-B*5701 Typing
Order Code:
HLATYP
Test Summary
Abacavir therapy for HIV treatment is associated with significant drug hypersensitivity in approximately 8% of recipients, with retrospective studies indicating a strong association with the HLA-B*5701 Allele. HLA-B*5701 typing can be used for genetic risk stratification prior to initiation of Abacavir therapy.
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
5 mL whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
3 mL
Instructions
A separate EDTA (lavender-top) tube must be submitted for all HLA testing. If submitted with any other test requiring an EDTA tube, please submit a separate tube for this test. Do not open tube.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 7 days
- Refrigerated: 7 days
- Frozen: Unacceptable
Limitations
A negative result does not rule out the risk for ABC HSR and clinical vigilance should be maintained regardless of the result. The frequency of HLA/-B5701 is approximately 8% in Caucasians and 2% in African Americans.
Other Acceptable Specimens
Whole blood collected in: sodium heparin (green-top) tube, EDTA (pink-top), EDTA (royal blue-top), or sodium citrate (light blue-top) tube
Unacceptable Specimens
Received frozen • Clotted
Order Code
HLATYP
EPIC (Premier) Code
LAB3312
Includes
Additional testing by Sequence Based Typing (SBT) may be performed when necessary.
CPT Code
- 81381
Billing Code
- 670053
CPT Statement
Methodology
Polymerase Chain Reaction (PCR) • Sequence Specific Oligonucleotide Probes (SSO)
FDA Status
Typing performed by using AS-PCR with reflex to the FDA-cleared LABType® SSO Kit. The AS-PCR portion of the test was developed and its performance characteristics have been determined by Quest Diagnostics.
Physician Attestation of Informed Consent
This germline genetic test requires physician attestation that patient consent has been received if ordering medical facility is located in AK, DE, FL, GA, IA, MA, MN, NV, NJ, NY, OR, SD or VT or test is performed in MA.
Testing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153
14225 Newbrook Drive
Chantilly VA, 20153
Department
Reference Testing
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Daily; Report available: 6 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
5 mL whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
3 mL
Instructions
A separate EDTA (lavender-top) tube must be submitted for all HLA testing. If submitted with any other test requiring an EDTA tube, please submit a separate tube for this test. Do not open tube.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 7 days
- Refrigerated: 7 days
- Frozen: Unacceptable
Limitations
A negative result does not rule out the risk for ABC HSR and clinical vigilance should be maintained regardless of the result. The frequency of HLA/-B5701 is approximately 8% in Caucasians and 2% in African Americans.
Other Acceptable Specimens
Whole blood collected in: sodium heparin (green-top) tube, EDTA (pink-top), EDTA (royal blue-top), or sodium citrate (light blue-top) tube
Unacceptable Specimens
Received frozen • Clotted
Result Information
Methodology
Polymerase Chain Reaction (PCR) • Sequence Specific Oligonucleotide Probes (SSO)
Testing Laboratory
N/A
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Daily; Report available: 6 days