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Test Directory

HIV-1 RNA, Quantitative, Real-Time PCR

Order Code

HIV1QT

Test Summary

This test is intended for use in conjunction with clinical presentation and other laboratory markers of disease progress for the clinical management of HIV-1 infected patients. The test can be used to assess patient prognosis by measuring the baseline HIV-1 RNA level or to monitor the effects of antiretroviral therapy by measuring changes in EDTA plasma HIV-1 RNA levels during the course of antiretroviral treatment.

Aliases

  • N/A

Specimen Collection

Special Instructions

N/A

Preferred Specimen

3 mL plasma collected in an EDTA (lavender-top) tube

Minimum Volume

1 mL

Instructions

Do not freeze whole blood or any samples stored in primary tube. Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation separate plasma from cells within 24 hours of collection by centrifugation. Follow manufacturer’s instructions for collection tube handling.

Patient Preparation

N/A

Storage

N/A

Transport Temperature

Frozen

Specimen Stability

  • Room temperature = Unacceptable
  • Refrigerated 6 days
  • Frozen 84 days

Limitations

N/A

Other Acceptable Specimens

Plasma collected in PPT (white-top) tube

Unacceptable Specimens

Received room temperature

Order Code

HIV1QT

EPIC (Premier) Code

LAB5325

Includes

N/A

CPT Code

  • 87536

Billing Code

  • 670003

CPT Statement

Methodology

Real-Time Polymerase Chain Reaction (RT-PCR)

FDA Status

FDA Approved

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153

Department

Reference Testing

Reference Range

HIV 1 RNA, QN PCR Not Detected copies/mL
HIV 1 RNA, QN PCR Not Detected Log copies/mL
This test was performed using Real-Time Polymerase Chain Reaction.
Reportable Range: 20 copies/mL to 10,000,000 copies/mL (1.30 log copies/mL to 7.00 log copies/mL)

Setup Schedule / Expected Turnaround Time

Monday - Saturday; Report available: 1 day

Specimen Collection

Special Instructions

N/A

Preferred Specimen

3 mL plasma collected in an EDTA (lavender-top) tube

Minimum Volume

1 mL

Instructions

Do not freeze whole blood or any samples stored in primary tube. Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation separate plasma from cells within 24 hours of collection by centrifugation. Follow manufacturer’s instructions for collection tube handling.

Patient Preparation

N/A

Storage

N/A

Transport Temperature

Frozen

Specimen Stability

  • Room temperature = Unacceptable
  • Refrigerated 6 days
  • Frozen 84 days

Limitations

N/A

Other Acceptable Specimens

Plasma collected in PPT (white-top) tube

Unacceptable Specimens

Received room temperature

Billing

CPT Code

  • 87536

Billing Code

  • 670003

CPT Statement

Result Information

Methodology

Real-Time Polymerase Chain Reaction (RT-PCR)

Testing Laboratory

N/A

Reference Range

HIV 1 RNA, QN PCR Not Detected copies/mL
HIV 1 RNA, QN PCR Not Detected Log copies/mL
This test was performed using Real-Time Polymerase Chain Reaction.
Reportable Range: 20 copies/mL to 10,000,000 copies/mL (1.30 log copies/mL to 7.00 log copies/mL)

Setup Schedule / Expected Turnaround Time

Monday - Saturday; Report available: 1 day