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Test Directory

Test Directory Hemoglobin, Free, Plasma

Hemoglobin, Free, Plasma

Test Summary

The presence of free hemoglobin in plasma is an indication of intravascular hemolysis resulting from numerous conditions, among which include paroxysmal nocturnal hemoglobinuria (PNH), sickle-cell disease (SCD), thalassemias, hereditary spherocytosis, microangiopathic hemolytic anemias, pyruvate kinase deficiency, ABO mismatch transfusion reaction, cardiopulmonary bypass or mechanical heart valve-induced anemia. Reference: JAMA. 2005;293(13):1653-1662

Aliases

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 mL plasma collected in a sodium heparin (green-top) tube

Minimum Volume

0.2 mL

Instructions

Centrifuge plasma within 1 hour of collection. Transfer the plasma to a sterile, plastic, screw-cap vial.

Patient Preparation

N/A

Storage

Plastic screw-cap vial

Transport Temperature

Room Temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 7 days
Frozen: Unacceptable

Limitations

N/A

Other Acceptable Specimens

Lithium heparin (green-top) tube

Unacceptable Specimens

Received frozen

Order Code

HGBFR

EPIC (Premier) Code

LAB206

Includes

N/A

CPT Code

83051

Billing Code

670043

CPT Statement

Methodology

Colorimetric (C)

FDA Status

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153

Department

Reference Testing

Reference Range

<8.4 mg/dL

Setup Schedule / Expected Turnaround Time

Tuesday - Saturday; Report available: 1 day