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FISH, B-Cell Chronic Lymphocytic Leukemia Panel

Test Summary

⁠⁠⁠⁠⁠⁠⁠This test is performed to detect the rearrangements of 6q21 (SEC63), 6q23 (MYB), ATM (11q22.3), centromere 12 (D12Z3), 13q14.3 (DLEU), 13q34 (LAMP1) and TP53 (17p13.1) regions, by FISH (fluorescence in situ hybridization). This assay is useful for prognostic assessment for chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL).

Aliases

  • N/A

Specimen Collection

Special Instructions

N/A

Preferred Specimen

3 mL bone marrow or 5 mL whole blood collected a sodium heparin (green-top) tube

Minimum Volume

1 mL bone marrow • 3 mL whole blood

Instructions

Clinical history and reason for referral are required with test order. Prior therapy and transplant history should be provided with test order.

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Note: If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.

Patient Preparation

N/A

Storage

N/A

Transport Temperature

Room temperature

Specimen Stability

  • Room temperature: See Collection Instructions
  • Refrigerated: See Collection Instructions
  • Frozen: See Collection Instructions

Limitations

N/A

Other Acceptable Specimens

Bone marrow or whole blood collected in: Sodium heparin (royal blue-top) tube or sodium heparin lead-free (tan-top) tube • 5x5mm lymph node tissue collected in Culture Transport Medium

Unacceptable Specimens

N/A

Order Code

FSHBCL

EPIC (Premier) Code

LAB5250

Includes

N/A

CPT Code

  • 88271 (x6)
  • 88275 (x3)

Billing Code

  • 671060
  • 671061

CPT Statement

Methodology

Fluorescence In Situ Hybridization (FISH)

FDA Status

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153

Department

Reference Testing

Reference Range

See Laboratory Report

Setup Schedule / Expected Turnaround Time

Daily; Report available: 8 days

Specimen Collection

Special Instructions

N/A

Preferred Specimen

3 mL bone marrow or 5 mL whole blood collected a sodium heparin (green-top) tube

Minimum Volume

1 mL bone marrow • 3 mL whole blood

Instructions

Clinical history and reason for referral are required with test order. Prior therapy and transplant history should be provided with test order.

Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Note: If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.

Patient Preparation

N/A

Storage

N/A

Transport Temperature

Room temperature

Specimen Stability

  • Room temperature: See Collection Instructions
  • Refrigerated: See Collection Instructions
  • Frozen: See Collection Instructions

Limitations

N/A

Other Acceptable Specimens

Bone marrow or whole blood collected in: Sodium heparin (royal blue-top) tube or sodium heparin lead-free (tan-top) tube • 5x5mm lymph node tissue collected in Culture Transport Medium

Unacceptable Specimens

N/A

Billing

CPT Code

  • 88271 (x6)
  • 88275 (x3)

Billing Code

  • 671060
  • 671061

CPT Statement

Result Information

Methodology

Fluorescence In Situ Hybridization (FISH)

Testing Laboratory

N/A

Reference Range

See Laboratory Report

Setup Schedule / Expected Turnaround Time

Daily; Report available: 8 days