FISH, FGFR1, 8p11-12
Test Summary
FISH testing with the FGFR1 probe allows detection of translocations involving FGFR1 and another chromosome region (usually a tyrosine kinase coding gene). Fusion of FGFR1 with a tyrosine kinase coding gene leads to kinase activation.
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
3 mL bone marrow or 5 mL whole blood collected in sodium heparin (green-top) tube
Minimum Volume
1 mL bone marrow • 3 mL whole blood
Instructions
Clinical history/reason for referral is required with test order. Prior therapy and transplant history should be provided with test order.
1-3 mL bone marrow in transport medium or 3-5 mL whole blood in a sodium heparin tube (green top, dark/royal blue top, tan top). Formalin fixed paraffin embedded tumor biopsy or 5 mm x 5 mm fresh tumor biopsy in transport medium.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
1-3 mL bone marrow in transport medium or 3-5 mL whole blood in a sodium heparin tube (green top, dark/royal blue top, tan top). Formalin fixed paraffin embedded tumor biopsy or 5 mm x 5 mm fresh tumor biopsy in transport medium.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: See instructions
- Refrigerated: See instructions
- Frozen: See instructions
Limitations
N/A
Other Acceptable Specimens
Bone marrow or whole blood collected in: sodium heparin (royal blue-top), or lead-free (tan-top) tube • Bone marrow submitted in culture transport media • 5x5mm fresh tumor tissue in transport medium • 5x5mm tumor tissue in formalin-fixed, paraffin-embedded tissue block
Unacceptable Specimens
N/A
Order Code
FGFFSH
EPIC (Premier) Code
LAB6336
Includes
N/A
CPT Code
- 88271 (x2)
- 88275
Billing Code
- 670294
- 670295
CPT Statement
Methodology
Fluorescence In Situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153
14225 Newbrook Drive
Chantilly VA, 20153
Department
Reference Testing
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Daily; Report available: 5 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
3 mL bone marrow or 5 mL whole blood collected in sodium heparin (green-top) tube
Minimum Volume
1 mL bone marrow • 3 mL whole blood
Instructions
Clinical history/reason for referral is required with test order. Prior therapy and transplant history should be provided with test order.
1-3 mL bone marrow in transport medium or 3-5 mL whole blood in a sodium heparin tube (green top, dark/royal blue top, tan top). Formalin fixed paraffin embedded tumor biopsy or 5 mm x 5 mm fresh tumor biopsy in transport medium.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
1-3 mL bone marrow in transport medium or 3-5 mL whole blood in a sodium heparin tube (green top, dark/royal blue top, tan top). Formalin fixed paraffin embedded tumor biopsy or 5 mm x 5 mm fresh tumor biopsy in transport medium.
Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: See instructions
- Refrigerated: See instructions
- Frozen: See instructions
Limitations
N/A
Other Acceptable Specimens
Bone marrow or whole blood collected in: sodium heparin (royal blue-top), or lead-free (tan-top) tube • Bone marrow submitted in culture transport media • 5x5mm fresh tumor tissue in transport medium • 5x5mm tumor tissue in formalin-fixed, paraffin-embedded tissue block
Unacceptable Specimens
N/A
Result Information
Methodology
Fluorescence In Situ Hybridization (FISH)
Testing Laboratory
N/A
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Daily; Report available: 5 days