FISH, Myeloid Neoplasms, MECOM (EVI1), 3q26.2
Order Code:
EVI1
Test Summary
Disruption of the human entropic virus integration site (EVI1 or MECOM) gene located at chromosome band 3q26.2 is associated with myeloid neoplasms and is indicative of a poor prognosis. EVI1 rearrangements are especially frequent in myeloid blast crisis that evolves through tyrosine kinase inhibitors. It has been associated with dysplasia of megakaryocytes and alters differentiation of other myeloid cells. Associated rearrangements include inv(3)(q21q26), t(3;3)q21;q26), t(3;21)(q26;q22) and t(3;12)(q26;p13).
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
3 mL bone marrow or 5 mL whole blood collected in a sodium heparin (green-top) tube
Minimum Volume
1 mL bone marrow • 3 mL whole blood
Instructions
1-3 mL bone marrow in a sodium heparin (green-top) tube is preferred. Bone marrow or peripheral blood in sodium heparin tube (green top, dark/royal blue, tan top) are acceptable. Ship at room temperature.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Limitations
N/A
Other Acceptable Specimens
Bone marrow collected in: sodium heparin lead-free (tan-top), or sodium heparin (royal blue-top) tube
Whole blood (not NY approved) collected in: sodium heparin (green-top) tube, sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube
Whole blood (not NY approved) collected in: sodium heparin (green-top) tube, sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube
Unacceptable Specimens
N/A
Order Code
EVI1
EPIC (Premier) Code
LAB5355
Includes
N/A
CPT Code
- 88271 (x3)
- 88275 - Please note: Whole blood is not New York approved for patient testing.
Billing Code
- 670286
- 670287
CPT Statement
Methodology
Fluorescence In Situ Hybridization (FISH)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153
14225 Newbrook Drive
Chantilly VA, 20153
Department
Reference Testing
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Daily; Report available: 6 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
3 mL bone marrow or 5 mL whole blood collected in a sodium heparin (green-top) tube
Minimum Volume
1 mL bone marrow • 3 mL whole blood
Instructions
1-3 mL bone marrow in a sodium heparin (green-top) tube is preferred. Bone marrow or peripheral blood in sodium heparin tube (green top, dark/royal blue, tan top) are acceptable. Ship at room temperature.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
If results are not possible, the test order may be canceled and replaced with a Cytogenetics Communication.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.
Limitations
N/A
Other Acceptable Specimens
Bone marrow collected in: sodium heparin lead-free (tan-top), or sodium heparin (royal blue-top) tube
Whole blood (not NY approved) collected in: sodium heparin (green-top) tube, sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube
Whole blood (not NY approved) collected in: sodium heparin (green-top) tube, sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube
Unacceptable Specimens
N/A
Billing
CPT Code
- 88271 (x3)
- 88275 - Please note: Whole blood is not New York approved for patient testing.
Billing Code
- 670286
- 670287
CPT Statement
Result Information
Methodology
Fluorescence In Situ Hybridization (FISH)
Testing Laboratory
N/A
Reference Range
See Laboratory Report
Setup Schedule / Expected Turnaround Time
Daily; Report available: 6 days