Acquired Thrombotic Profile
Order Code:
ACTHP
Test Summary
Lupus Anticoagulants are acquired circulating inhibitors, usually IgG immunoglobulin, but sometimes IgM or IgA. Due to the wide variability of phospholipid activity among patients, there is no single test that will detect the presence of Lupus Anticoagulants. Therefore, multiple assays using reagents with varying amounts of phospholipids are used to detect the presence of Lupus Anticoagulants. Venous as well as arterial thrombosis may occur in patients with Lupus Anticoagulants. Lupus Anticoagulant antibodies occur in various clinical conditions, including autoimmune diseases, unexplained thrombosis and recurrent spontaneous abortions and fetal demise.
Aliases
- Includes a homocysteine, Factor VIII Activity, Lupus Anticoagulant Assay, Platelet count, PT/INR, APTT, Anticardiolipin Antibodies, Phosphatidylserine Antibodies, Beta 2 Glycoprotein Antibodies.
Specimen Collection
Special Instructions
See Link and Print "Drug List for Special Coagualtion Tests"
Preferred Specimen
(2) K2EDTA (lavender top) tubes
2 SST (speckled top) tubes OR GST (gold top) tubes
6 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tubes
2 SST (speckled top) tubes OR GST (gold top) tubes
6 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tubes
Minimum Volume
1 K2EDTA (lavender top) tube
3 Frozen Capped aliquots of at least 0.5mL serum (Polypropylene tubes recommended)
AND
4 Frozen Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
3 Frozen Capped aliquots of at least 0.5mL serum (Polypropylene tubes recommended)
AND
4 Frozen Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
Instructions
Use standard venipuncture and disposal techniques.
SST/GST tube: Avoid hemolysis.
1. Invert a minimum of 5 times, and then allow clotting in an upright position for 30 minutes.
2. Centrifuge the SST/GST for at 3000 rpm for 10 minutes.
3. Spun SST/GST tube remains at refrigerated temperature (2-8 degrees C) for transport up to 72 hours.
4. Serum must be removed from original SST/GST tube and frozen at <-20 degrees C in plastic polypropylene aliquot tubes within 72 hours of collection. Refer to the Minimum Volume section above for the number of aliquots required per test.
SST/GST tube: Avoid hemolysis.
1. Invert a minimum of 5 times, and then allow clotting in an upright position for 30 minutes.
2. Centrifuge the SST/GST for at 3000 rpm for 10 minutes.
3. Spun SST/GST tube remains at refrigerated temperature (2-8 degrees C) for transport up to 72 hours.
4. Serum must be removed from original SST/GST tube and frozen at <-20 degrees C in plastic polypropylene aliquot tubes within 72 hours of collection. Refer to the Minimum Volume section above for the number of aliquots required per test.
Patient Preparation
N/A
Storage
SST (speckled top) tube or GST (gold top) tube
OR
Frozen- Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
OR
Frozen- Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
Transport Temperature
Room Temperature: Unacceptable
Refrigerated: SST (speckled top) tube or GST (gold top) tube
Frozen: Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
Refrigerated: SST (speckled top) tube or GST (gold top) tube
Frozen: Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
Specimen Stability
- Room Temperature: Unacceptable
- Refrigerated: SST (speckled top) or GST (gold top) tube <
- 72 hours
- Frozen: Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended) at <
- -20⁰C for <
- 60 days
- at <
- -70⁰C for 12months
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Hemolysis, lipemia, incorrect specimen type, insufficient sample volume, improper storage conditions, exceeds specimen stability guidelines, contaminated sample
Order Code
ACTHP
EPIC (Premier) Code
LAB4205
Includes
Includes a homocysteine, Factor VIII Activity, Lupus Anticoagulant Assay, Platelet count, PT/INR, APTT, Anticardiolipin Antibodies, Phosphatidlyserine Antibodies, Beta 2 Glycoprotein Antibodies.
CPT Code
- 83090
- 85240
- 85670
- 85613
- 85347
- 85610
- 85730
- 86147(3)
- 86148(3)
- 86146(3)
- 85049(2)
Billing Code
- 500331
- 300440
- 300490
- 300485
- 300461
- 300482
- 300492
- 300511
- 300512
- 300510
- 300434
CPT Statement
Methodology
Clot Based, ELISA
FDA Status
FDA Approved; PNP test is performed if not FDA approved.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Department
Hematology
Reference Range
See Report
Setup Schedule / Expected Turnaround Time
Tuesday, Friday; SCOAG; Report available: 1 - 4 days
Specimen Collection
Special Instructions
See Link and Print "Drug List for Special Coagualtion Tests"
Preferred Specimen
(2) K2EDTA (lavender top) tubes
2 SST (speckled top) tubes OR GST (gold top) tubes
6 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tubes
2 SST (speckled top) tubes OR GST (gold top) tubes
6 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tubes
Minimum Volume
1 K2EDTA (lavender top) tube
3 Frozen Capped aliquots of at least 0.5mL serum (Polypropylene tubes recommended)
AND
4 Frozen Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
3 Frozen Capped aliquots of at least 0.5mL serum (Polypropylene tubes recommended)
AND
4 Frozen Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
Instructions
Use standard venipuncture and disposal techniques.
SST/GST tube: Avoid hemolysis.
1. Invert a minimum of 5 times, and then allow clotting in an upright position for 30 minutes.
2. Centrifuge the SST/GST for at 3000 rpm for 10 minutes.
3. Spun SST/GST tube remains at refrigerated temperature (2-8 degrees C) for transport up to 72 hours.
4. Serum must be removed from original SST/GST tube and frozen at <-20 degrees C in plastic polypropylene aliquot tubes within 72 hours of collection. Refer to the Minimum Volume section above for the number of aliquots required per test.
SST/GST tube: Avoid hemolysis.
1. Invert a minimum of 5 times, and then allow clotting in an upright position for 30 minutes.
2. Centrifuge the SST/GST for at 3000 rpm for 10 minutes.
3. Spun SST/GST tube remains at refrigerated temperature (2-8 degrees C) for transport up to 72 hours.
4. Serum must be removed from original SST/GST tube and frozen at <-20 degrees C in plastic polypropylene aliquot tubes within 72 hours of collection. Refer to the Minimum Volume section above for the number of aliquots required per test.
Patient Preparation
N/A
Storage
SST (speckled top) tube or GST (gold top) tube
OR
Frozen- Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
OR
Frozen- Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
Transport Temperature
Room Temperature: Unacceptable
Refrigerated: SST (speckled top) tube or GST (gold top) tube
Frozen: Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
Refrigerated: SST (speckled top) tube or GST (gold top) tube
Frozen: Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
Specimen Stability
- Room Temperature: Unacceptable
- Refrigerated: SST (speckled top) or GST (gold top) tube <
- 72 hours
- Frozen: Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended) at <
- -20⁰C for <
- 60 days
- at <
- -70⁰C for 12months
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Hemolysis, lipemia, incorrect specimen type, insufficient sample volume, improper storage conditions, exceeds specimen stability guidelines, contaminated sample
Billing
CPT Code
- 83090
- 85240
- 85670
- 85613
- 85347
- 85610
- 85730
- 86147(3)
- 86148(3)
- 86146(3)
- 85049(2)
Billing Code
- 500331
- 300440
- 300490
- 300485
- 300461
- 300482
- 300492
- 300511
- 300512
- 300510
- 300434
CPT Statement
Result Information
Methodology
Clot Based, ELISA
Testing Laboratory
N/A
Reference Range
See Report
Setup Schedule / Expected Turnaround Time
Tuesday, Friday; SCOAG; Report available: 1 - 4 days