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Test Directory

Catecholamines, Fractionated, 24-Hour Urine with Creatinine

Test Summary

The three catecholamines (norepinephrine, epinephrine and dopamine) are the principal secretory products of neural tissue. Clinically, the measurement of circulating catecholamines is valuable in the diagnosis of catecholamine secreting tumors associated chiefly with hypertension (pheochromocytomas, neuroblastomas and gangliomas) and with the evaluation of orthostatic hypotension.

Aliases

  • N/A

Specimen Collection

Special Instructions

N/A

Preferred Specimen

10 mL aliquot from 24-hour urine preserved with 25 mL 6N HCl, collected in plastic 24-hour urine container

Minimum Volume

5 mL

Instructions

Collect 24-hour urine with 25 mL 6N HCL to maintain a pH < or = 3. If no preservative is used, specimen must be stored refrigerated during collection but shipped frozen and pH must be < or = 6. Record 24-hour urine volume and patient's age on test request form and on urine container.

Patient Preparation

It is preferable for the patient to be off medications for three days prior to collection. However, common antihypertensives (diuretics, ACE Inhibitors, calcium channel blockers, alpha and beta blockers) cause minimal or no interference. Patient should avoid alcohol, coffee, tea, tobacco and strenuous exercise prior to collection.

Storage

N/A

Transport Temperature

Preserved urine: Room temperature
Unpreserved urine: Frozen

Specimen Stability

  • Preserved
  • Room temperature: 7 days
  • Refrigerated: 30 days
  • Frozen: 49 days
  • Unpreserved
  • Room temperature: Unacceptable
  • Refrigerated: Unacceptable
  • Frozen: 30 days

Limitations

Recent surgery, traumatic injury, upright posture, cold, anxiety, pain, clonidine withdrawal, and concurrent acute or chronic illness may produce elevated results..

Other Acceptable Specimens

10 mL aliquot from 24-hour urine, unpreserved, with pH < or = 6, collected in plastic 24-hour urine container

Unacceptable Specimens

Preserved urine received refrigerated with pH >3 • Unpreserved urine received frozen with pH >6

Order Code

CAT24U

EPIC (Premier) Code

LAB3956

Includes

Creatinine, Norepinephrine, Epinephrine, Dopamine, and Total Catecholamines (calculated)

CPT code 81050 may be added at an additional charge for volume measurement

CPT Code

  • 82384
  • 82570

Billing Code

  • 670625
  • 670626

CPT Statement

Methodology

High Performance Liquid Chromatography (HPLC)

FDA Status

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153

Department

Reference Testing

Reference Range

See Laboratory Report

Setup Schedule / Expected Turnaround Time

Monday - Saturday morning; Report available: 3 days

Specimen Collection

Special Instructions

N/A

Preferred Specimen

10 mL aliquot from 24-hour urine preserved with 25 mL 6N HCl, collected in plastic 24-hour urine container

Minimum Volume

5 mL

Instructions

Collect 24-hour urine with 25 mL 6N HCL to maintain a pH < or = 3. If no preservative is used, specimen must be stored refrigerated during collection but shipped frozen and pH must be < or = 6. Record 24-hour urine volume and patient's age on test request form and on urine container.

Patient Preparation

It is preferable for the patient to be off medications for three days prior to collection. However, common antihypertensives (diuretics, ACE Inhibitors, calcium channel blockers, alpha and beta blockers) cause minimal or no interference. Patient should avoid alcohol, coffee, tea, tobacco and strenuous exercise prior to collection.

Storage

N/A

Transport Temperature

Preserved urine: Room temperature
Unpreserved urine: Frozen

Specimen Stability

  • Preserved
  • Room temperature: 7 days
  • Refrigerated: 30 days
  • Frozen: 49 days
  • Unpreserved
  • Room temperature: Unacceptable
  • Refrigerated: Unacceptable
  • Frozen: 30 days

Limitations

Recent surgery, traumatic injury, upright posture, cold, anxiety, pain, clonidine withdrawal, and concurrent acute or chronic illness may produce elevated results..

Other Acceptable Specimens

10 mL aliquot from 24-hour urine, unpreserved, with pH < or = 6, collected in plastic 24-hour urine container

Unacceptable Specimens

Preserved urine received refrigerated with pH >3 • Unpreserved urine received frozen with pH >6

Billing

CPT Code

  • 82384
  • 82570

Billing Code

  • 670625
  • 670626

CPT Statement

Result Information

Methodology

High Performance Liquid Chromatography (HPLC)

Testing Laboratory

N/A

Reference Range

See Laboratory Report

Setup Schedule / Expected Turnaround Time

Monday - Saturday morning; Report available: 3 days