Functional Protein C Activity
Order Code:
FUNPRC
Test Summary
This assay detects a deficiency in Protein C that maybe otherwise normal when tested using Antigenic and Chromogenic methods. Protein C deficiencies exist as both acquired and inherited disorders. Acquired Protein C deficiencies are found in hepatic disorders, oral anticoagulant therapies and DIC. Congenital Protein C deficiencies are characterized by recurrent venous thrombosis and exist in different types.
Aliases
- N/A
Specimen Collection
Special Instructions
See Link and Print "Drug List for Special Coagualtion Tests"
Preferred Specimen
1 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tube
Minimum Volume
2 Frozen Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
Instructions
Use standard venipuncture and disposal techniques.
Collect the required quantity of Sodium Citrate (light blue top) tubes.
Call Transportation for a STAT pick-up at 937-297-8262. If unable to obtain STAT pick-up then proceed to the Sodium Citrate (light blue top) tubes instructions below.
Sodium Citrate (light blue top) tube(s): Process immediately (stable only 4 hours), following instructions below:
Note: We recommend aliquot tubes to be made of polypropylene plastic as this material is safe to freeze at ≤-70 degrees C.
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (≤5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat, and combine all plasma into a 15mL plastic conical centrifuge tube. Note: If the plasma shows any hemolysis, it must be recollected.
3. Centrifuge the plasma in the 15mL plastic conical centrifuge tube for a second time as in step 1.
4. In one smooth motion, carefully and quickly pour the centrifuged plasma into a second 15mL plastic conical centrifuge tube. A small amount of plasma along with a red cell button should remain on the bottom of the original conical tube if the pour was executed correctly. Note: This quick-pour method prevents platelet transfer and contamination.
5. Perform a platelet count on the well mixed double-spun pooled plasma from the second 15 mL plastic conical centrifuge tube. Platelet count must be ≤ 5/kmm3. Notes: If the platelet count is greater than 5/kmm3 then repeat the process starting at step 3. If unable to perform a platelet count then indicate on requisition.
6. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
7. Finally, place all aliquots into a ≤-20°C freezer within 4 hours. Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.
Collect the required quantity of Sodium Citrate (light blue top) tubes.
Call Transportation for a STAT pick-up at 937-297-8262. If unable to obtain STAT pick-up then proceed to the Sodium Citrate (light blue top) tubes instructions below.
Sodium Citrate (light blue top) tube(s): Process immediately (stable only 4 hours), following instructions below:
Note: We recommend aliquot tubes to be made of polypropylene plastic as this material is safe to freeze at ≤-70 degrees C.
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (≤5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat, and combine all plasma into a 15mL plastic conical centrifuge tube. Note: If the plasma shows any hemolysis, it must be recollected.
3. Centrifuge the plasma in the 15mL plastic conical centrifuge tube for a second time as in step 1.
4. In one smooth motion, carefully and quickly pour the centrifuged plasma into a second 15mL plastic conical centrifuge tube. A small amount of plasma along with a red cell button should remain on the bottom of the original conical tube if the pour was executed correctly. Note: This quick-pour method prevents platelet transfer and contamination.
5. Perform a platelet count on the well mixed double-spun pooled plasma from the second 15 mL plastic conical centrifuge tube. Platelet count must be ≤ 5/kmm3. Notes: If the platelet count is greater than 5/kmm3 then repeat the process starting at step 3. If unable to perform a platelet count then indicate on requisition.
6. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
7. Finally, place all aliquots into a ≤-20°C freezer within 4 hours. Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.
Patient Preparation
N/A
Storage
Whole blood Sodium Citrate (light blue top) tube(s)
OR
Frozen - Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
OR
Frozen - Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
Transport Temperature
Room Temperature: Whole blood Sodium Citrate (light blue top) tube(s)
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma
Specimen Stability
- Room Temperature: Whole blood Sodium Citrate (light blue top) tube(s) <
- 4 hours
- Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
- Frozen: Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended) 3 months at &le
- -70degreesC
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Rejected for hemolysis, clotted, improper fill, incorrect specimen type, thawed frozen aliquots, refrigerated plasma or blood, expired test tubes, >4 hours old if not processed into separated frozen plasma vials.
Order Code
FUNPRC
EPIC (Premier) Code
LAB4443
Includes
N/A
CPT Code
- 85303
Billing Code
- 300456
CPT Statement
Methodology
Clot Based
FDA Status
FDA approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Department
Hematology
Reference Range
See Report
Setup Schedule / Expected Turnaround Time
Wednesday; SCOAG; Report available: 1 - 4 days
Specimen Collection
Special Instructions
See Link and Print "Drug List for Special Coagualtion Tests"
Preferred Specimen
1 plastic (2.7mL) completely filled 3.2% Sodium Citrate (light blue top) tube
Minimum Volume
2 Frozen Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
Instructions
Use standard venipuncture and disposal techniques.
Collect the required quantity of Sodium Citrate (light blue top) tubes.
Call Transportation for a STAT pick-up at 937-297-8262. If unable to obtain STAT pick-up then proceed to the Sodium Citrate (light blue top) tubes instructions below.
Sodium Citrate (light blue top) tube(s): Process immediately (stable only 4 hours), following instructions below:
Note: We recommend aliquot tubes to be made of polypropylene plastic as this material is safe to freeze at ≤-70 degrees C.
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (≤5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat, and combine all plasma into a 15mL plastic conical centrifuge tube. Note: If the plasma shows any hemolysis, it must be recollected.
3. Centrifuge the plasma in the 15mL plastic conical centrifuge tube for a second time as in step 1.
4. In one smooth motion, carefully and quickly pour the centrifuged plasma into a second 15mL plastic conical centrifuge tube. A small amount of plasma along with a red cell button should remain on the bottom of the original conical tube if the pour was executed correctly. Note: This quick-pour method prevents platelet transfer and contamination.
5. Perform a platelet count on the well mixed double-spun pooled plasma from the second 15 mL plastic conical centrifuge tube. Platelet count must be ≤ 5/kmm3. Notes: If the platelet count is greater than 5/kmm3 then repeat the process starting at step 3. If unable to perform a platelet count then indicate on requisition.
6. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
7. Finally, place all aliquots into a ≤-20°C freezer within 4 hours. Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.
Collect the required quantity of Sodium Citrate (light blue top) tubes.
Call Transportation for a STAT pick-up at 937-297-8262. If unable to obtain STAT pick-up then proceed to the Sodium Citrate (light blue top) tubes instructions below.
Sodium Citrate (light blue top) tube(s): Process immediately (stable only 4 hours), following instructions below:
Note: We recommend aliquot tubes to be made of polypropylene plastic as this material is safe to freeze at ≤-70 degrees C.
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (≤5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat, and combine all plasma into a 15mL plastic conical centrifuge tube. Note: If the plasma shows any hemolysis, it must be recollected.
3. Centrifuge the plasma in the 15mL plastic conical centrifuge tube for a second time as in step 1.
4. In one smooth motion, carefully and quickly pour the centrifuged plasma into a second 15mL plastic conical centrifuge tube. A small amount of plasma along with a red cell button should remain on the bottom of the original conical tube if the pour was executed correctly. Note: This quick-pour method prevents platelet transfer and contamination.
5. Perform a platelet count on the well mixed double-spun pooled plasma from the second 15 mL plastic conical centrifuge tube. Platelet count must be ≤ 5/kmm3. Notes: If the platelet count is greater than 5/kmm3 then repeat the process starting at step 3. If unable to perform a platelet count then indicate on requisition.
6. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
7. Finally, place all aliquots into a ≤-20°C freezer within 4 hours. Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.
Patient Preparation
N/A
Storage
Whole blood Sodium Citrate (light blue top) tube(s)
OR
Frozen - Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
OR
Frozen - Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
Transport Temperature
Room Temperature: Whole blood Sodium Citrate (light blue top) tube(s)
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma
Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
Frozen: Capped aliquots of Sodium Citrate plasma
Specimen Stability
- Room Temperature: Whole blood Sodium Citrate (light blue top) tube(s) <
- 4 hours
- Refrigerated: Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
- Frozen: Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended) 3 months at &le
- -70degreesC
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Rejected for hemolysis, clotted, improper fill, incorrect specimen type, thawed frozen aliquots, refrigerated plasma or blood, expired test tubes, >4 hours old if not processed into separated frozen plasma vials.
Result Information
Methodology
Clot Based
Testing Laboratory
N/A
Reference Range
See Report
Setup Schedule / Expected Turnaround Time
Wednesday; SCOAG; Report available: 1 - 4 days