RET (10q11), FISH, Tissue
Order Code:
RETF
Test Summary
Identifying RET gene rearrangements in patients with late-stage, lung adenocarcinomas that are negative for EGFR mutations and ALK rearrangements
Aliases
- Non-small cell lung cancer (NSCLC)
Specimen Collection
Special Instructions
N/A
Preferred Specimen
Formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used. Paraffin-embedded specimens can be from any anatomic location (skin, soft tissue, lymph node, etc). Bone specimens that have been decalcified will be attempted for testing, but the success rate is approximately 50%.
Minimum Volume
Slides: 1 Hematoxylin and eosin stained and 2 unstained
Instructions
A pathology report is required for testing to be performed. If not provided, appropriate testing and/or interpretation may be compromised or delayed. Acceptable pathology reports include working drafts, preliminary pathology, or surgical pathology reports.
Patient Preparation
N/A
Storage
Room temperature: indefinite
Refrigerated: indefinite
Frozen: NA
Refrigerated: indefinite
Frozen: NA
Transport Temperature
Ambient
Specimen Stability
- N/A
Limitations
N/A
Other Acceptable Specimens
Tissue slides: 1 Hematoxylin and eosin stained and 4 unstained. Submit 4 consecutive unstained, positively charged, unbaked slides with 5 micron-thick sections of the tumor tissue and 1 slide stained with hematoxylin and eosin.
Unacceptable Specimens
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.
Order Code
RETF
EPIC (Premier) Code
LAB6472
Includes
N/A
CPT Code
- 88271x2
- 88291 – DNA probe
- each (first probe set)
- Interpretation and report 88271x2 – DNA probe
- each additional probe set (if appropriate) 88271x1 – DNA probe
- each coverage for sets containing 3 probes (if appropriate) 88271x2 – DNA probe
- each coverage for sets containing 4 probes (if appropriate) 88271x3 – DNA probe
- each coverage for sets containing 5 probes (if appropriate) 88274 w/modifier 52 – Interphase in situ hybridization < 25 cells
- each probe set (if appropriate) 88274 – Interphase in situ hybridization 25 to 99 cells
- each probe set (if appropriate) 88275 – Interphase in situ hybridization 100 to 300 cells
- each probe set (if appropriate)
Billing Code
- 671157
- 671166
CPT Statement
Methodology
Fluorescence In Situ Hybridization (FISH)
FDA Status
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Mayo Clinic Laboratories
200 First Street SW
Rochester MN, 55905
200 First Street SW
Rochester MN, 55905
Department
Reference Testing
Reference Range
N/A
Setup Schedule / Expected Turnaround Time
Monday - Friday; Report available: 7 - 10 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
Formalin-fixed, paraffin-embedded (FFPE) tumor tissue block. Blocks prepared with alternative fixation methods may be acceptable; provide fixation method used. Paraffin-embedded specimens can be from any anatomic location (skin, soft tissue, lymph node, etc). Bone specimens that have been decalcified will be attempted for testing, but the success rate is approximately 50%.
Minimum Volume
Slides: 1 Hematoxylin and eosin stained and 2 unstained
Instructions
A pathology report is required for testing to be performed. If not provided, appropriate testing and/or interpretation may be compromised or delayed. Acceptable pathology reports include working drafts, preliminary pathology, or surgical pathology reports.
Patient Preparation
N/A
Storage
Room temperature: indefinite
Refrigerated: indefinite
Frozen: NA
Refrigerated: indefinite
Frozen: NA
Transport Temperature
Ambient
Specimen Stability
- N/A
Limitations
N/A
Other Acceptable Specimens
Tissue slides: 1 Hematoxylin and eosin stained and 4 unstained. Submit 4 consecutive unstained, positively charged, unbaked slides with 5 micron-thick sections of the tumor tissue and 1 slide stained with hematoxylin and eosin.
Unacceptable Specimens
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.
Billing
CPT Code
- 88271x2
- 88291 – DNA probe
- each (first probe set)
- Interpretation and report 88271x2 – DNA probe
- each additional probe set (if appropriate) 88271x1 – DNA probe
- each coverage for sets containing 3 probes (if appropriate) 88271x2 – DNA probe
- each coverage for sets containing 4 probes (if appropriate) 88271x3 – DNA probe
- each coverage for sets containing 5 probes (if appropriate) 88274 w/modifier 52 – Interphase in situ hybridization < 25 cells
- each probe set (if appropriate) 88274 – Interphase in situ hybridization 25 to 99 cells
- each probe set (if appropriate) 88275 – Interphase in situ hybridization 100 to 300 cells
- each probe set (if appropriate)
Billing Code
- 671157
- 671166
CPT Statement
Result Information
Methodology
Fluorescence In Situ Hybridization (FISH)
Testing Laboratory
N/A
Reference Range
N/A
Setup Schedule / Expected Turnaround Time
Monday - Friday; Report available: 7 - 10 days