Primary Membranous Nephropathy Diagnostic Cascade, Serum
Test Summary
Distinguishing primary from secondary membranous nephropathy
using an algorithmic approach
Monitoring patients with membranous nephropathy at very low
antibody titers
Screening for anti-phospholipase A2 receptor antibodies
Aliases
- Anti-PLA2R, PLA2R, Anti-THSD7A, Thrombospondin, THSD7
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL serum
Minimum Volume
1 mL serum from a serum separator tube
Instructions
Centrifuge and aliquot serum into plastic vial within 2 hours of collection.
Patient Preparation
N/A
Storage
Plastic screw-cap vial
Transport Temperature
Refrigerated
Specimen Stability
- Room temperature: 8 hours
- Refrigerated: 14 days
- Frozen: 14 days
Limitations
N/A
Other Acceptable Specimens
serum from a red top tube
Unacceptable Specimens
Gross hemolysis
Order Code
PMND1
EPIC (Premier) Code
LAB6815
Includes
The phospholipase A2 receptor (PLA2R) enzyme-linked
immunosorbent assay (ELISA) is initially performed.
If the PLA2R ELISA result is less than 20, then the PLA2R
immunofluorescence testing will be performed at an
additional charge.
If the PLA2R immunofluorescence result is negative,
thrombospondin type-1 domain-containing 7A (THSD7A) antibody
testing will be performed at an additional charge.
immunosorbent assay (ELISA) is initially performed.
If the PLA2R ELISA result is less than 20, then the PLA2R
immunofluorescence testing will be performed at an
additional charge.
If the PLA2R immunofluorescence result is negative,
thrombospondin type-1 domain-containing 7A (THSD7A) antibody
testing will be performed at an additional charge.
CPT Code
- 83520
Billing Code
- 650063
CPT Statement
Methodology
EURO: Enzyme-Linked Immunosorbent Assay (ELISA), PLA2I, THSD7: Indirect Immunofluorescence Assay (IFA)
FDA Status
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Mayo Clinic Laboratories
200 First Street SW
Rochester MN, 55905
200 First Street SW
Rochester MN, 55905
Department
Reference Testing
Reference Range
N/A
Setup Schedule / Expected Turnaround Time
Monday, Wednesday, Friday; Reports available: 3 - 7 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL serum
Minimum Volume
1 mL serum from a serum separator tube
Instructions
Centrifuge and aliquot serum into plastic vial within 2 hours of collection.
Patient Preparation
N/A
Storage
Plastic screw-cap vial
Transport Temperature
Refrigerated
Specimen Stability
- Room temperature: 8 hours
- Refrigerated: 14 days
- Frozen: 14 days
Limitations
N/A
Other Acceptable Specimens
serum from a red top tube
Unacceptable Specimens
Gross hemolysis
Result Information
Methodology
EURO: Enzyme-Linked Immunosorbent Assay (ELISA), PLA2I, THSD7: Indirect Immunofluorescence Assay (IFA)
Testing Laboratory
N/A
Reference Range
N/A
Setup Schedule / Expected Turnaround Time
Monday, Wednesday, Friday; Reports available: 3 - 7 days