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Neuromyelitis Optica (NMO)/Aquaporin-4-IgG Fluorescence-Activated Cell Sorting (FACS) Assay, Serum

Test Summary

Diagnosis of a neuromyelitis optica spectrum disorder (NMOSD)
Diagnosis of autoimmune AQP4 channelopathy
Diagnosis of neuromyelitis optica (NMO)
Distinguishing NMOSD from multiple sclerosis early in the course of disease

Aliases

  • NMO (Neruomyelitis Optica) NMO-IgG AQP4 Aquaporin Devic'
  • s Antibody Transverse Myelitis Antibody Optic Neuritis Antibody Vision Loss Antibody

Specimen Collection

Special Instructions

N/A

Preferred Specimen

3 mL serum from a red top tube

Minimum Volume

2 mL

Instructions

N/A

Patient Preparation

N/A

Storage

Pour off tube

Transport Temperature

Refrigerated

Specimen Stability

  • Room Temperature: 72 hours
  • Refrigerated: 28 days
  • Frozen: 28 days

Limitations

AQP4-IgG antibodies may drop below detectable levels in setting of therapies for acute attack (IV methylprednisolone or plasmapheresis) or attack prevention (immunosuppressants).

Other Acceptable Specimens

Serum from a serum separator tube

Unacceptable Specimens

N/A

Order Code

NMOFS

EPIC (Premier) Code

LAB6451

Includes

N/A

CPT Code

  • 86053
  • 86053- NMO/AQP4-IgG FACS titer (if appropriate)

Billing Code

  • 650026

CPT Statement

Methodology

Flow Cytometry (FC)

FDA Status

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Mayo Clinic Laboratories
200 First Street SW
Rochester MN, 55905

Department

Reference Testing

Reference Range

N/A

Setup Schedule / Expected Turnaround Time

Monday, Tuesday, Thursday; Report available: 5 - 8 days

Specimen Collection

Special Instructions

N/A

Preferred Specimen

3 mL serum from a red top tube

Minimum Volume

2 mL

Instructions

N/A

Patient Preparation

N/A

Storage

Pour off tube

Transport Temperature

Refrigerated

Specimen Stability

  • Room Temperature: 72 hours
  • Refrigerated: 28 days
  • Frozen: 28 days

Limitations

AQP4-IgG antibodies may drop below detectable levels in setting of therapies for acute attack (IV methylprednisolone or plasmapheresis) or attack prevention (immunosuppressants).

Other Acceptable Specimens

Serum from a serum separator tube

Unacceptable Specimens

N/A

Billing

CPT Code

  • 86053
  • 86053- NMO/AQP4-IgG FACS titer (if appropriate)

Billing Code

  • 650026

CPT Statement

Result Information

Methodology

Flow Cytometry (FC)

Testing Laboratory

N/A

Reference Range

N/A

Setup Schedule / Expected Turnaround Time

Monday, Tuesday, Thursday; Report available: 5 - 8 days