Cardiolipin IGM Antibody
Test Summary
Anti-cardiolipin (aCL) antibodies are frequently found in patients with systemic lupus erythematosus (SLE). They are also found in patients with other autoimmune diseases, as well as in some individuals with no apparent previous underlying disease. Elevated levels of aCL antibodies have been reported to be significantly associated with the presence of both venous and arterial thrombosis, thrombocytopenia, and recurrent fetal loss. The term “anti-phospholipid syndrome” (APS) has been introduced to describe patients who present these clinical manifestations, in association with aCL antibodies or the lupus anticoagulant. Beta 2 Glycoprotein Antibodies are anti-phospholipid antibodies and are a heterogeneous group of immunoglobulins that bind to several anionic phospholipids, including cardiolipin and phosphatidylserine. High serum levels of anti-phospholipid antibodies are frequently detected in patients with autoimmune (e.g., SLE) and non-autoimmune diseases, as well as in apparently healthy individuals. These antibodies have been associated with an increased risk for recurrent arterial and venous thrombotic events, thrombocytopenia, and fetal loss. These manifestations are the main features of the anti-phospholipid syndrome (APS).
Aliases
- ACA IgM
- AntiCardiolipin Antibodies IgM
Specimen Collection
Special Instructions
Preferred Specimen
Minimum Volume
Instructions
SST/GST tube: Avoid hemolysis.
1. Invert a minimum of 5 times, and then allow clotting in an upright position for 30 minutes.
2. Centrifuge the SST/GST for at 3000 rpm for 10 minutes.
3. Spun SST/GST tube remains at refrigerated temperature (2-8 degrees C) for transport up to 72 hours.
4. Serum must be removed from original SST/GST tube and frozen at <-20 degrees C in plastic polypropylene aliquot tubes within 72 hours of collection. Refer to the Minimum Volume section above for the number of aliquots required per test.
Patient Preparation
Storage
OR
Frozen- Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
Transport Temperature
Refrigerated: SST (speckled top) tube or GST (gold top) tube
Frozen: Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
Specimen Stability
- Room Temperature: Unacceptable
- Refrigerated: SST (speckled top) or GST (gold top) tube <
- 72 hours
- Frozen: Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)at <
- -20⁰C for <
- 60 days
- at <
- -70⁰C for 12months
Limitations
Other Acceptable Specimens
Unacceptable Specimens
Order Code
EPIC (Premier) Code
Includes
CPT Code
- 86147
Billing Code
- 300511
CPT Statement
Methodology
FDA Status
Physician Attestation of Informed Consent
Testing Laboratory
2308 Sandridge Drive
Moraine OH, 45439
Department
Reference Range
Setup Schedule / Expected Turnaround Time
Specimen Collection
Special Instructions
Preferred Specimen
Minimum Volume
Instructions
SST/GST tube: Avoid hemolysis.
1. Invert a minimum of 5 times, and then allow clotting in an upright position for 30 minutes.
2. Centrifuge the SST/GST for at 3000 rpm for 10 minutes.
3. Spun SST/GST tube remains at refrigerated temperature (2-8 degrees C) for transport up to 72 hours.
4. Serum must be removed from original SST/GST tube and frozen at <-20 degrees C in plastic polypropylene aliquot tubes within 72 hours of collection. Refer to the Minimum Volume section above for the number of aliquots required per test.
Patient Preparation
Storage
OR
Frozen- Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
Transport Temperature
Refrigerated: SST (speckled top) tube or GST (gold top) tube
Frozen: Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)
Specimen Stability
- Room Temperature: Unacceptable
- Refrigerated: SST (speckled top) or GST (gold top) tube <
- 72 hours
- Frozen: Capped aliquots of at least 0.5 mL serum (polypropylene tubes recommended)at <
- -20⁰C for <
- 60 days
- at <
- -70⁰C for 12months