T-Spot TB
Test Summary
The T-Spot test is based upon a cell-mediated immune response in TB-infected individuals. The T cells of the patient are stimulated with Mycobacterium tuberculosis antigens. If the patient has previously been exposed to Mycobacterium tuberculosis, the exposure to these antigens in the T-Spot assay stimulate the patient's T cells to secrete the cytokine interferon-γ. The presence of the interferon-γ is then detected using an EIA method. According to the CDC, this assay may be used as a substitute for a TB skin test. It is more specific than the TB skin test, will detect latent TB, and a positive result is obtained in about 85% of the patients with active TB.
Aliases
- T Spot AB
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 Sodium Heparin (dark green top) tube. Adults and children over 10 years: 6 mL Whole Blood (minimum). Children 2 - 9 years of age: 4 mL Whole Blood (minimum). Children up to 2 years of age: 2 mL Whole Blood (minimum).
Minimum Volume
N/A
Instructions
Avoid hemolysis. Store at room temperature. DO NOT refrigerate.
Specimen should arrive at CompuNet's main lab before midnight (on the collection date).
Specimen should arrive at CompuNet's main lab before midnight (on the collection date).
Patient Preparation
N/A
Storage
Sodium Heparin (dark green top) tube
Transport Temperature
Room Temperature
Specimen should arrive at CompuNet's Main Lab before midnight (on the collection date).
Specimen should arrive at CompuNet's Main Lab before midnight (on the collection date).
Specimen Stability
- Room Temperature = 30 hours
- Refrigerated = unacceptable
- Frozen = unacceptable
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Rejected if received refrigerated or frozen. Rejected for low white cell count.
Order Code
TSPOT
EPIC (Premier) Code
LAB4457
Includes
N/A
CPT Code
- 86481
Billing Code
- 300000
CPT Statement
Methodology
Lymphocyte Stimulation with Immunoassay
FDA Status
Approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Reference Range
N/A
Setup Schedule / Expected Turnaround Time
Monday - Sunday; BS; Report available: 2 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 Sodium Heparin (dark green top) tube. Adults and children over 10 years: 6 mL Whole Blood (minimum). Children 2 - 9 years of age: 4 mL Whole Blood (minimum). Children up to 2 years of age: 2 mL Whole Blood (minimum).
Minimum Volume
N/A
Instructions
Avoid hemolysis. Store at room temperature. DO NOT refrigerate.
Specimen should arrive at CompuNet's main lab before midnight (on the collection date).
Specimen should arrive at CompuNet's main lab before midnight (on the collection date).
Patient Preparation
N/A
Storage
Sodium Heparin (dark green top) tube
Transport Temperature
Room Temperature
Specimen should arrive at CompuNet's Main Lab before midnight (on the collection date).
Specimen should arrive at CompuNet's Main Lab before midnight (on the collection date).
Specimen Stability
- Room Temperature = 30 hours
- Refrigerated = unacceptable
- Frozen = unacceptable
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Rejected if received refrigerated or frozen. Rejected for low white cell count.
Result Information
Methodology
Lymphocyte Stimulation with Immunoassay
Testing Laboratory
N/A
Reference Range
N/A
Setup Schedule / Expected Turnaround Time
Monday - Sunday; BS; Report available: 2 days