Quanti-FERON® TB-Gold
Test Summary
The QuantiFERON test is based upon a cell-mediated immune response in TB-infected individuals. The T cells of the patient are stimulated with Mycobacterium tuberculosis antigens. If the patient has previously been exposed to Mycobacterium tuberculosis, the exposure to these antigens in the QuantiFERON assay stimulate the patient's T cells to secrete the cytokine interferon-γ. The presence of the interferon-γ is then detected using an EIA method. According to the CDC, this assay may be used as a substitute for a TB skin test. It is more specific than the TB skin test, will detect latent TB, and a positive result is obtained in about 85% of the patients with active TB.
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
QuantiFERON In-Tube collection tubes required.
In-Tube collection tubes are a set 4 tubes that are used specifically for collection for Quanti-FERON® TB-Gold testing.
In-Tube collection tubes are a set 4 tubes that are used specifically for collection for Quanti-FERON® TB-Gold testing.
Minimum Volume
N/A
Instructions
NOTE: Outpatients from a doctor's office must go to a PSC to have the sample collected.
Hospitalized patients will be drawn by the phlebotomy staff at the hospital.
QuantiFERON(TM)In-Tube collection tubes REQUIRED. In-Tube collection tubes are a set 4 tubes that are used specifically for collection for QuantiFERON(TM) testing.
Draw blood in all 4 tubes, shake vigorously (until frothy), and send to laboratory at room temperature (DO NOT REFRIGERATE). Must be processed within 16 hours of collection.
DO NOT SPIN THE TUBES
Hospitalized patients will be drawn by the phlebotomy staff at the hospital.
QuantiFERON(TM)In-Tube collection tubes REQUIRED. In-Tube collection tubes are a set 4 tubes that are used specifically for collection for QuantiFERON(TM) testing.
Draw blood in all 4 tubes, shake vigorously (until frothy), and send to laboratory at room temperature (DO NOT REFRIGERATE). Must be processed within 16 hours of collection.
DO NOT SPIN THE TUBES
Patient Preparation
N/A
Storage
Quanti-FERON® TB-Gold in-tube collection tubes
Transport Temperature
Room Temperature
Specimen Stability
- Room Temperature = 16 hours
- Refrigerated = unacceptable
- Frozen = unacceptable
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Reject specimen if older than 16 hours post collection, refrigerated or frozen
Order Code
QTF
EPIC (Premier) Code
LAB1401
Includes
N/A
CPT Code
- 86480
Billing Code
- 300553
CPT Statement
Methodology
Lymphocyte Stimulation with Immunoassay
FDA Status
FDA Approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Department
Molecular/Serology
Reference Range
A positive and negative control are performed with each patient sample.
If these controls are within the acceptable limits, any value <0.35 IU/ML is interpreted as negative and any value >=0.35 IU/ML is interpreted as positive. If the positive and negative control are outside the acceptable limits, the results are interpreted as indeterminate.
If these controls are within the acceptable limits, any value <0.35 IU/ML is interpreted as negative and any value >=0.35 IU/ML is interpreted as positive. If the positive and negative control are outside the acceptable limits, the results are interpreted as indeterminate.
Setup Schedule / Expected Turnaround Time
Collect Samples: Monday - Sunday. Testing Performed: Tuesday, Thursday, and Saturday or Sunday; BS; Report available: 4 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
QuantiFERON In-Tube collection tubes required.
In-Tube collection tubes are a set 4 tubes that are used specifically for collection for Quanti-FERON® TB-Gold testing.
In-Tube collection tubes are a set 4 tubes that are used specifically for collection for Quanti-FERON® TB-Gold testing.
Minimum Volume
N/A
Instructions
NOTE: Outpatients from a doctor's office must go to a PSC to have the sample collected.
Hospitalized patients will be drawn by the phlebotomy staff at the hospital.
QuantiFERON(TM)In-Tube collection tubes REQUIRED. In-Tube collection tubes are a set 4 tubes that are used specifically for collection for QuantiFERON(TM) testing.
Draw blood in all 4 tubes, shake vigorously (until frothy), and send to laboratory at room temperature (DO NOT REFRIGERATE). Must be processed within 16 hours of collection.
DO NOT SPIN THE TUBES
Hospitalized patients will be drawn by the phlebotomy staff at the hospital.
QuantiFERON(TM)In-Tube collection tubes REQUIRED. In-Tube collection tubes are a set 4 tubes that are used specifically for collection for QuantiFERON(TM) testing.
Draw blood in all 4 tubes, shake vigorously (until frothy), and send to laboratory at room temperature (DO NOT REFRIGERATE). Must be processed within 16 hours of collection.
DO NOT SPIN THE TUBES
Patient Preparation
N/A
Storage
Quanti-FERON® TB-Gold in-tube collection tubes
Transport Temperature
Room Temperature
Specimen Stability
- Room Temperature = 16 hours
- Refrigerated = unacceptable
- Frozen = unacceptable
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Reject specimen if older than 16 hours post collection, refrigerated or frozen
Result Information
Methodology
Lymphocyte Stimulation with Immunoassay
Testing Laboratory
N/A
Reference Range
A positive and negative control are performed with each patient sample.
If these controls are within the acceptable limits, any value <0.35 IU/ML is interpreted as negative and any value >=0.35 IU/ML is interpreted as positive. If the positive and negative control are outside the acceptable limits, the results are interpreted as indeterminate.
If these controls are within the acceptable limits, any value <0.35 IU/ML is interpreted as negative and any value >=0.35 IU/ML is interpreted as positive. If the positive and negative control are outside the acceptable limits, the results are interpreted as indeterminate.
Setup Schedule / Expected Turnaround Time
Collect Samples: Monday - Sunday. Testing Performed: Tuesday, Thursday, and Saturday or Sunday; BS; Report available: 4 days