Lyme Antibody w/ Western Blot Confirmation
Test Summary
Lyme disease is caused by a bacterium borrelia burgdorferi and is transmitted by ticks. EIA is the screening test with high sensitivity for antibody detection. Western blot testing qualitatively examines with high specificity antibodies in a patient's specimen. Western blot testing is appropriate for confirming a detected EIA test result.
Aliases
- B Burgdorferi
- Borrelia B Earl
- Borrelia Burgdorferi (serum)
- Borrelia Burgdorferi Antibodies
- Lyme (serum)
- Lyme Disease
- Lime Disease AB Screen (Total), EIA
- Lyme Early
- Lyme IgG &
- IgM
- &nbs
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL serum (minimum 0.5 mL)
Minimum Volume
N/A
Instructions
SST or Red top: Avoid hemolysis. Invert a minimum of 5 times, then allow to clot in an upright position for 30 minutes. Centrifuge the tube for at least 10 minutes. Keep tightly stoppered.
Patient Preparation
N/A
Storage
SST (speckled top), Red top , Plastic Vial (transfer) tube
Transport Temperature
Refrigerated (preferred)
Specimen Stability
- Room Temperature = n/a
- Refrigerated = 7 days
- Frozen = at least 7 days
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Rejected for gross hemolysis, and lipemia. Microbial contamination may interfere. Plasma will be rejected
Order Code
LYME
EPIC (Premier) Code
LAB4342
Includes
Western blot confirmation CPT code(s) 86617x2 performed when screen is positive at an additional charge.
CPT Code
- 86618 (reflex 86617(2))
Billing Code
- 300000
CPT Statement
Methodology
Enzyme Immunoassay (EIA)
FDA Status
Approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Reference Range
| SEX | AGE | Report Normals | Units |
| N/M/F | 133Y | < OR = 0.90 Negative | |
| 0.91 - 1.09 Equivocal | |||
| > OR = 1.10 Positive | |||
| Due to recommendations by the food and drug administration (FDA), all positive or equivocal Borrelia Burgdorferi Antibody EIA (screening) tests will be followed by the western blot. The screening test for B. Burgdorferi has a low predictive value for a negative result when used to detect early infection and a low predictive value of a positive result when exposure history, symptoms, and clinical findings are not consistent with Lyme disease. Positive or equivocal results should not be interpreted as true positives until a second-step testing of the specimen is done using a method that is more specific for antibodies to B. Burgdorferi (e.g. Western Blot). | |||
Setup Schedule / Expected Turnaround Time
Tuesday - Thursday; BS; Report available: 6 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL serum (minimum 0.5 mL)
Minimum Volume
N/A
Instructions
SST or Red top: Avoid hemolysis. Invert a minimum of 5 times, then allow to clot in an upright position for 30 minutes. Centrifuge the tube for at least 10 minutes. Keep tightly stoppered.
Patient Preparation
N/A
Storage
SST (speckled top), Red top , Plastic Vial (transfer) tube
Transport Temperature
Refrigerated (preferred)
Specimen Stability
- Room Temperature = n/a
- Refrigerated = 7 days
- Frozen = at least 7 days
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Rejected for gross hemolysis, and lipemia. Microbial contamination may interfere. Plasma will be rejected
Result Information
Methodology
Enzyme Immunoassay (EIA)
Testing Laboratory
N/A
Reference Range
| SEX | AGE | Report Normals | Units |
| N/M/F | 133Y | < OR = 0.90 Negative | |
| 0.91 - 1.09 Equivocal | |||
| > OR = 1.10 Positive | |||
| Due to recommendations by the food and drug administration (FDA), all positive or equivocal Borrelia Burgdorferi Antibody EIA (screening) tests will be followed by the western blot. The screening test for B. Burgdorferi has a low predictive value for a negative result when used to detect early infection and a low predictive value of a positive result when exposure history, symptoms, and clinical findings are not consistent with Lyme disease. Positive or equivocal results should not be interpreted as true positives until a second-step testing of the specimen is done using a method that is more specific for antibodies to B. Burgdorferi (e.g. Western Blot). | |||
Setup Schedule / Expected Turnaround Time
Tuesday - Thursday; BS; Report available: 6 days