DIC Profile
Test Summary
Identify the presence and follow the course of disseminated intravascular coagulation (DIC)
Aliases
- Platelet Count Activated Partial Thromboplastin Time (aPTT) Prothrombin Time Thrombin Time Plasminogen Activity Fibrinogen Quantitative D-Dimer
Specimen Collection
Special Instructions
See Link and Print "Drug List for Special Coagualtion Tests"
Preferred Specimen
1 K2EDTA lavender top tube
3 plastic (2.7mL) completely 3.2% filled Sodium Citrate (light blue top) tubes
3 plastic (2.7mL) completely 3.2% filled Sodium Citrate (light blue top) tubes
Minimum Volume
1 K2EDTA 3mL or 4mL lavender top tube (Minimum fill of 1mL)
or K2EDTA Microtainer® lavender top
4 Frozen Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
or K2EDTA Microtainer® lavender top
4 Frozen Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
Instructions
Use standard venipuncture and disposal techniques.
Collect the required quantity of Sodium Citrate (light blue top) tubes.
If unable to process specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.
K2EDTA lavender top tube: Gently invert 10 times immediately at time of collection (do not spin) then maintain at room temperature or refrigerate.
Sodium Citrate (light blue top) tubes: Process immediately (stable only 4 hours), following instructions below:
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat, and combine all plasma into a plastic conical centrifuge tube.
Note: If the plasma shows any hemolysis, it must be recollected.
3. Centrifuge the plasma in the plastic conical centrifuge tube for a second time as in step 1.
4. In one smooth motion, carefully and quickly pour the centrifuged plasma into a second plastic conical centrifuge tube. A small amount of plasma along with a red cell button should remain on the bottom of the original conical tube if the pour was executed correctly.
Note: This quick-pour method prevents platelet transfer and contamination.
5. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
6. Finally, place all aliquots into a <-20°C freezer within 4 hours.
Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.
Collect the required quantity of Sodium Citrate (light blue top) tubes.
If unable to process specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.
K2EDTA lavender top tube: Gently invert 10 times immediately at time of collection (do not spin) then maintain at room temperature or refrigerate.
Sodium Citrate (light blue top) tubes: Process immediately (stable only 4 hours), following instructions below:
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat, and combine all plasma into a plastic conical centrifuge tube.
Note: If the plasma shows any hemolysis, it must be recollected.
3. Centrifuge the plasma in the plastic conical centrifuge tube for a second time as in step 1.
4. In one smooth motion, carefully and quickly pour the centrifuged plasma into a second plastic conical centrifuge tube. A small amount of plasma along with a red cell button should remain on the bottom of the original conical tube if the pour was executed correctly.
Note: This quick-pour method prevents platelet transfer and contamination.
5. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
6. Finally, place all aliquots into a <-20°C freezer within 4 hours.
Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.
Patient Preparation
N/A
Storage
K2EDTA lavender top tube Whole blood Sodium Citrate (light blue top) tubes
OR
Frozen - Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
OR
Frozen - Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
Transport Temperature
Room Temperature: K2EDTA lavender top tube; Whole Blood
Specimen Stability
- Room Temperature: K2EDTA lavender top tube <
- 24 hours
- Whole blood Sodium Citrate (light blue top) tubes <
- 4 hours
- Refrigerated: K2EDTA lavender top tube <
- 48hours
- Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
- Frozen: Unacceptable for K2EDTA lavender top tube
- Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended) <
- 14 days at <
- &minus
- 20 degrees C
- or <
- 6 months at <
- -70 degrees C
Limitations
Platelet count: Platelet clumping, if present an estimate will be given i.e. decreased, adequate or increased. A numeral result is not possible if clumping is present.
Anti-coagulant medications
Anti-coagulant medications
Other Acceptable Specimens
N/A
Unacceptable Specimens
K2EDTA Lavender Rejected for gross hemolysis, clotted, <1mL, non-EDTA tube type, >48hours old and received frozen. Sodium Citrate Plasma: Rejected for hemolysis, clotted, improper fill, incorrect specimen type, thawed frozen aliquots, refrigerated plasma or blood, expired test tubes, >4 hours old if not processed into separated frozen plasma vials.
Order Code
DICPRO
EPIC (Premier) Code
LAB10120
Includes
Platelet Count
Activated Partial Thromboplastin Time (aPTT)
Prothrombin Time
Thrombin Time
Plasminogen Activity
Fibrinogen
Quantitative D-Dimer
Activated Partial Thromboplastin Time (aPTT)
Prothrombin Time
Thrombin Time
Plasminogen Activity
Fibrinogen
Quantitative D-Dimer
CPT Code
- 85049
- 85670
- 85420
- 85384
- 85610
- 85730
- 85379
Billing Code
- 300482
- 300492
- 300434
- 300468
- 300472
- 300490
- 300467
CPT Statement
Methodology
Automated Cell counting, Clot Based, Immunoturbidimetric
FDA Status
FDA approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Department
Hematology
Reference Range
See Report
Setup Schedule / Expected Turnaround Time
Daily; Thrombin/Plasminogen on Tuesday, Thursday SCOAG; Report available: 1 - 4 days
Specimen Collection
Special Instructions
See Link and Print "Drug List for Special Coagualtion Tests"
Preferred Specimen
1 K2EDTA lavender top tube
3 plastic (2.7mL) completely 3.2% filled Sodium Citrate (light blue top) tubes
3 plastic (2.7mL) completely 3.2% filled Sodium Citrate (light blue top) tubes
Minimum Volume
1 K2EDTA 3mL or 4mL lavender top tube (Minimum fill of 1mL)
or K2EDTA Microtainer® lavender top
4 Frozen Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
or K2EDTA Microtainer® lavender top
4 Frozen Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
Instructions
Use standard venipuncture and disposal techniques.
Collect the required quantity of Sodium Citrate (light blue top) tubes.
If unable to process specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.
K2EDTA lavender top tube: Gently invert 10 times immediately at time of collection (do not spin) then maintain at room temperature or refrigerate.
Sodium Citrate (light blue top) tubes: Process immediately (stable only 4 hours), following instructions below:
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat, and combine all plasma into a plastic conical centrifuge tube.
Note: If the plasma shows any hemolysis, it must be recollected.
3. Centrifuge the plasma in the plastic conical centrifuge tube for a second time as in step 1.
4. In one smooth motion, carefully and quickly pour the centrifuged plasma into a second plastic conical centrifuge tube. A small amount of plasma along with a red cell button should remain on the bottom of the original conical tube if the pour was executed correctly.
Note: This quick-pour method prevents platelet transfer and contamination.
5. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
6. Finally, place all aliquots into a <-20°C freezer within 4 hours.
Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.
Collect the required quantity of Sodium Citrate (light blue top) tubes.
If unable to process specimens within 4 hours of collection then call CompuNet transportation for a STAT pick-up at (937) 297-8262 option 1.
K2EDTA lavender top tube: Gently invert 10 times immediately at time of collection (do not spin) then maintain at room temperature or refrigerate.
Sodium Citrate (light blue top) tubes: Process immediately (stable only 4 hours), following instructions below:
1. Centrifuge capped Sodium Citrate (light blue top) tubes at 3000 rpm for 10 minutes, or at speed and time required to consistently produce Platelet Poor Plasma (<5/kmm3).
2. Carefully remove the plasma from all Sodium Citrate (light blue top) tubes; avoid disturbing the WBC/buffy coat, and combine all plasma into a plastic conical centrifuge tube.
Note: If the plasma shows any hemolysis, it must be recollected.
3. Centrifuge the plasma in the plastic conical centrifuge tube for a second time as in step 1.
4. In one smooth motion, carefully and quickly pour the centrifuged plasma into a second plastic conical centrifuge tube. A small amount of plasma along with a red cell button should remain on the bottom of the original conical tube if the pour was executed correctly.
Note: This quick-pour method prevents platelet transfer and contamination.
5. Aliquot at least 1mL of plasma into each aliquot tubes labeled “plasma.” Refer to the Minimum Volume section above for the number of aliquots required per test.
6. Finally, place all aliquots into a <-20°C freezer within 4 hours.
Note: Aliquots must remain frozen until they are picked up by the CompuNet courier. Call CompuNet transportation department if frozen specimen boxes are needed.
Patient Preparation
N/A
Storage
K2EDTA lavender top tube Whole blood Sodium Citrate (light blue top) tubes
OR
Frozen - Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
OR
Frozen - Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended)
Transport Temperature
Room Temperature: K2EDTA lavender top tube; Whole Blood
Specimen Stability
- Room Temperature: K2EDTA lavender top tube <
- 24 hours
- Whole blood Sodium Citrate (light blue top) tubes <
- 4 hours
- Refrigerated: K2EDTA lavender top tube <
- 48hours
- Unacceptable for Sodium Citrate (light blue top) tubes whole blood or capped plasma aliquots
- Frozen: Unacceptable for K2EDTA lavender top tube
- Capped aliquots of Sodium Citrate plasma (Polypropylene tubes recommended) <
- 14 days at <
- &minus
- 20 degrees C
- or <
- 6 months at <
- -70 degrees C
Limitations
Platelet count: Platelet clumping, if present an estimate will be given i.e. decreased, adequate or increased. A numeral result is not possible if clumping is present.
Anti-coagulant medications
Anti-coagulant medications
Other Acceptable Specimens
N/A
Unacceptable Specimens
K2EDTA Lavender Rejected for gross hemolysis, clotted, <1mL, non-EDTA tube type, >48hours old and received frozen. Sodium Citrate Plasma: Rejected for hemolysis, clotted, improper fill, incorrect specimen type, thawed frozen aliquots, refrigerated plasma or blood, expired test tubes, >4 hours old if not processed into separated frozen plasma vials.
Billing
CPT Code
- 85049
- 85670
- 85420
- 85384
- 85610
- 85730
- 85379
Billing Code
- 300482
- 300492
- 300434
- 300468
- 300472
- 300490
- 300467
CPT Statement
Result Information
Methodology
Automated Cell counting, Clot Based, Immunoturbidimetric
Testing Laboratory
N/A
Reference Range
See Report
Setup Schedule / Expected Turnaround Time
Daily; Thrombin/Plasminogen on Tuesday, Thursday SCOAG; Report available: 1 - 4 days