Cryoglobulin Screen with Reflex to Cryoglobulin Panel
Test Summary
Cryoglobulins are proteins that precipitate from serum at temperatures below 37C degrees. Most precipitate when serum is cooled at 4C degrees, but some gel even at room temperature. Many of the cryoglobulins have the ability to fix complement and initiate an inflammatory reaction similar to classic antigen-antibody complexes and thus, probably play a significant role in the pathogenesis of viral disease with the production of lesions of vasculitis and glomerulonephritis. Cryoglobulin have been detected in a wide variety of lymphoproliferative, autoimmune and infectious diseases.
Aliases
- N/A
Specimen Collection
Special Instructions
Preferred Specimen
Minimum Volume
Instructions
Stability restrictions: Sample has only 72 hr stability. Only draw Monday through Thursday.
Collect 20 mL of fasting whole blood specimen in a red-top tube (no gel).
SPECIMEN MUST BE PLACED IN A PREWARMED THERMOS 37O DEGREES CONTAINING SAND IMMEDIATLEY AFTER COLLECTION.
10 mL serum required: 3.5 mL minimum.
Avoid hemolysis.
Allow serum sample to clot for one (1) hour in a 37°C water bath, oven, incubator, or heat block.
After clotting, centrifuge the specimen for 10 minutes at 3000 rpm utilizing one of the 3 options below:
1) In a "warm" centrifuge at 37° C; or
2) In a bench-top centrifuge placed in a "warm room" at 37°C; or
3) At room temperature, in a bench-top centrifuge prewarmed by running it for 10 minutes at 2000 rpm. -After centrifuging, decant serum into a screw-capped vial -Transport at room temperature. Note: No other tests can be added to the tube; cryocrit and cryoglobulin evaluation only
Patient Preparation
Storage
Transport Temperature
Specimen Stability
- Room temperature: 72 hours
- Refrigerated: Unacceptable
- Frozen: Unacceptable
Limitations
Other Acceptable Specimens
Unacceptable Specimens
Order Code
EPIC (Premier) Code
Includes
CPT Code
- 82595
Billing Code
- 670990
CPT Statement
Methodology
FDA Status
Physician Attestation of Informed Consent
Testing Laboratory
2308 Sandridge Drive
Moraine OH, 45439
Department
Reference Range
Setup Schedule / Expected Turnaround Time
Specimen Collection
Special Instructions
Preferred Specimen
Minimum Volume
Instructions
Stability restrictions: Sample has only 72 hr stability. Only draw Monday through Thursday.
Collect 20 mL of fasting whole blood specimen in a red-top tube (no gel).
SPECIMEN MUST BE PLACED IN A PREWARMED THERMOS 37O DEGREES CONTAINING SAND IMMEDIATLEY AFTER COLLECTION.
10 mL serum required: 3.5 mL minimum.
Avoid hemolysis.
Allow serum sample to clot for one (1) hour in a 37°C water bath, oven, incubator, or heat block.
After clotting, centrifuge the specimen for 10 minutes at 3000 rpm utilizing one of the 3 options below:
1) In a "warm" centrifuge at 37° C; or
2) In a bench-top centrifuge placed in a "warm room" at 37°C; or
3) At room temperature, in a bench-top centrifuge prewarmed by running it for 10 minutes at 2000 rpm. -After centrifuging, decant serum into a screw-capped vial -Transport at room temperature. Note: No other tests can be added to the tube; cryocrit and cryoglobulin evaluation only
Patient Preparation
Storage
Transport Temperature
Specimen Stability
- Room temperature: 72 hours
- Refrigerated: Unacceptable
- Frozen: Unacceptable