Cryoglobulin Screen with Reflex to Cryoglobulin Panel
Order Code: CRYOG
Test Summary
Cryoglobulins are proteins that precipitate from serum at temperatures below 37C°. Most precipitate when serum is cooled at 4C °, but some gel even at room temperature. Many of the cryoglobulins have the ability to fix complement and initiate an inflammatory reaction similar to classic antigen-antibody complexes and thus, probably play a significant role in the pathogenesis of viral disease with the production of lesions of vasculitis and glomerulonephritis. Cryoglobulin have been detected in a wide variety of lymphoproliferative, autoimmune and infectious diseases.
Aliases
N/A
Specimen Collection
Special Instructions
Allow blood to clot for one (1) hour in a 37°C water bath, incubator, heat block, or heel warmer. After clotting, centrifuge the specimen as quickly as possible. It is not necessary to use a warm centrifuge but do not use a refrigerated one. Decant all of the serum into one transport vial (do not aliquot any of the cryoglobulin serum into any other tube). Transport at room temperature preferred.
Preferred Specimen
Collect 2 FULL red-top (no gel) tubes to yield 3 mL serum at laboratory
Minimum Volume
Collect 2 FULL red-top (no gel) tubes to yield 2 mL serum at laboratory
Instructions
MUST BE COLLECT AT SANDRIDGE, A HOSPITAL, OR PSC/CLIENT WITH INCUBATION REQUIREMENTS
Patient Preparation
Overnight fasting is required
Storage
Plastic screw-top tube
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 5 days
- Refrigerated: 7 days
- Frozen: 30 days
Limitations
If the sample is not kept at 37°C during clotting, the cryoglobulin, if present, can precipitate during centrifugation and be lost in the clot resulting in a false negative result or N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
- Gross hemolysis
- Lipemia
- Icteric
- Serum separator tube (SST)
Order Code
CRYOG
EPIC (Premier) Code
LAB5383
Includes
If cryoglobulin is present (positive), it is identified. Positives are semi-quantitated (as % cryoglobulin) by cryocrit. If Cryoglobulin Screen is positive, then Cryoglobulin Panel (Cryocrit Immunofixation, Cryocrit Immunodiffusion and Rheumatoid Factor) will be performed at an additional charge (CPT Code(s): 86334, 86329, 86431).
CPT Code
- 82595
Billing Code
- 670990
CPT Statement
Methodology
- Cryocrit
- Electrophoresis
- Immunodiffusion (ID)
- Immunofixation
- Rate Nephelometry
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano CA, 92675
33608 Ortega Highway
San Juan Capistrano CA, 92675
Department
Reference Testing
Reference Range
Negative in normal patients
Setup Schedule / Expected Turnaround Time
Sunday - Friday; Report available: 5 - 7 days
Specimen Collection
Special Instructions
Allow blood to clot for one (1) hour in a 37°C water bath, incubator, heat block, or heel warmer. After clotting, centrifuge the specimen as quickly as possible. It is not necessary to use a warm centrifuge but do not use a refrigerated one. Decant all of the serum into one transport vial (do not aliquot any of the cryoglobulin serum into any other tube). Transport at room temperature preferred.
Preferred Specimen
Collect 2 FULL red-top (no gel) tubes to yield 3 mL serum at laboratory
Minimum Volume
Collect 2 FULL red-top (no gel) tubes to yield 2 mL serum at laboratory
Instructions
MUST BE COLLECT AT SANDRIDGE, A HOSPITAL, OR PSC/CLIENT WITH INCUBATION REQUIREMENTS
Patient Preparation
Overnight fasting is required
Storage
Plastic screw-top tube
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 5 days
- Refrigerated: 7 days
- Frozen: 30 days
Limitations
If the sample is not kept at 37°C during clotting, the cryoglobulin, if present, can precipitate during centrifugation and be lost in the clot resulting in a false negative result or N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
- Gross hemolysis
- Lipemia
- Icteric
- Serum separator tube (SST)
Result Information
Methodology
- Cryocrit
- Electrophoresis
- Immunodiffusion (ID)
- Immunofixation
- Rate Nephelometry
Testing Laboratory
N/A
Reference Range
Negative in normal patients
Setup Schedule / Expected Turnaround Time
Sunday - Friday; Report available: 5 - 7 days