Enterovirus RNA, QL Real Time RT-PCR
Test Summary
Reverse transcriptase polymerase chain reaction (RT-PCR) is a highly sensitive method used to detect enterovirus (EV) RNA. This assay targets a conserved region of the EV genome which allows detection of nearly the entire group of human enteroviruses, including the coxsackieviruses, polioviruses, and the echoviruses. (It does not differentiate among the EV serotypes.)
The diagnosis of enterovirus infection should not rely solely upon the result of a RT-PCR assay. A negative RT-PCR result indicated the absence of enterovirus RNA in the sample tested and does not exclude the diagnosis of disease.
Aliases
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL CSF in sterile screw-cap container
Minimum Volume
0.4 mL CSF
Instructions
CSF must be sent in original tube or screw-cap container.
Do not put CSF into VTM.
Store refrigerated after collection.
Lower Respiratory Sources:
1.0 mL bronchial wash or bronchial lavage in a sterile container
Do not put CSF into VTM.
Store refrigerated after collection.
Lower Respiratory Sources:
1.0 mL bronchial wash or bronchial lavage in a sterile container
Patient Preparation
N/A
Storage
Sterile screw-cap container
Transport Temperature
Refrigerated
Specimen Stability
- Room Temperature = n/a
- Refrigerated = 3 days
- Frozen = 3 months
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
N/A
Order Code
ENTPCB
EPIC (Premier) Code
LAB5167
Includes
N/A
CPT Code
- 87498
Billing Code
- 300753
CPT Statement
Methodology
This test employs a real time reverse transcriptase polymerase chain reaction (RT-PCR) for the amplification of a conserved region sequence from enterovirus RNA.
FDA Status
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Department
Molecular/Serology
Reference Range
N/A
Setup Schedule / Expected Turnaround Time
Monday - Sunday; MD; Report available: 1 - 2 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL CSF in sterile screw-cap container
Minimum Volume
0.4 mL CSF
Instructions
CSF must be sent in original tube or screw-cap container.
Do not put CSF into VTM.
Store refrigerated after collection.
Lower Respiratory Sources:
1.0 mL bronchial wash or bronchial lavage in a sterile container
Do not put CSF into VTM.
Store refrigerated after collection.
Lower Respiratory Sources:
1.0 mL bronchial wash or bronchial lavage in a sterile container
Patient Preparation
N/A
Storage
Sterile screw-cap container
Transport Temperature
Refrigerated
Specimen Stability
- Room Temperature = n/a
- Refrigerated = 3 days
- Frozen = 3 months
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
N/A
Result Information
Methodology
This test employs a real time reverse transcriptase polymerase chain reaction (RT-PCR) for the amplification of a conserved region sequence from enterovirus RNA.
Testing Laboratory
N/A
Reference Range
N/A
Setup Schedule / Expected Turnaround Time
Monday - Sunday; MD; Report available: 1 - 2 days