ACTIN (Smooth Muscle) Antibody (IgG)
Test Summary
The presence of actin antibodies can be used in conjunction with clinical findings and other laboratory tests to aid in the diagnosis of autoimmune liver diseases such as autoimmune hepatitis (AIH) and primary biliary cirrhosis (PBC).
Aliases
- Anti Smooth Muscle
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL Serum SST (speckled top or gold top) tube
Minimum Volume
0.5 mL serum
Instructions
SST or Red top: Avoid hemolysis. Invert a minimum of 5 times, then allow to clot in an upright position for 30 minutes. Centrifuge the tube for at least 10 minutes. Keep tightly stoppered.
Patient Preparation
N/A
Storage
SST (speckled top), Red top , Plastic Vial (transfer) tube
Transport Temperature
Room Temperature or Refrigerated (preferred)
Specimen Stability
- Room Temperature = 24 hours
- Refrigerated = 7 days
- Frozen = at least 7 days
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
N/A
Order Code
ACAB
EPIC (Premier) Code
LAB365
Includes
N/A
CPT Code
- 86015
Billing Code
- 500000
CPT Statement
Methodology
Enzyme Immunoassay (EIA)
FDA Status
Approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Reference Range
| SEX | AGE | Report Normals |
| N/M/F | 1000Y | < 20 = Negative |
| 20 - 30 = Weak Positive | ||
| > 30 = High Positive | ||
| Anti-smooth muscle antibodies (ANTI-SMA) were previously tested for by indirect immunofluorescence (IF) using rat stomach as antigen. To enhance our testing specificity for autoimmune hepatitis type 1 (AIH TYPE 1), immunofluorescence has been replaced by an ELISA based assay to purify F-ACTIN. IgG antibodies to F-ACTIN are present in approximately 75% of patients with AIH TYPE 1, approximately 65% of patients with autoimmune Cholagitis, approximately 30% of patients with primary Biliary Cirrhosis, and approximately 2% of healthy controls. High values are closely correlated with AIH TYPE 1. | ||
Setup Schedule / Expected Turnaround Time
Monday, Wednesday, Friday; BS; Reports available: 3 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL Serum SST (speckled top or gold top) tube
Minimum Volume
0.5 mL serum
Instructions
SST or Red top: Avoid hemolysis. Invert a minimum of 5 times, then allow to clot in an upright position for 30 minutes. Centrifuge the tube for at least 10 minutes. Keep tightly stoppered.
Patient Preparation
N/A
Storage
SST (speckled top), Red top , Plastic Vial (transfer) tube
Transport Temperature
Room Temperature or Refrigerated (preferred)
Specimen Stability
- Room Temperature = 24 hours
- Refrigerated = 7 days
- Frozen = at least 7 days
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
N/A
Result Information
Methodology
Enzyme Immunoassay (EIA)
Testing Laboratory
N/A
Reference Range
| SEX | AGE | Report Normals |
| N/M/F | 1000Y | < 20 = Negative |
| 20 - 30 = Weak Positive | ||
| > 30 = High Positive | ||
| Anti-smooth muscle antibodies (ANTI-SMA) were previously tested for by indirect immunofluorescence (IF) using rat stomach as antigen. To enhance our testing specificity for autoimmune hepatitis type 1 (AIH TYPE 1), immunofluorescence has been replaced by an ELISA based assay to purify F-ACTIN. IgG antibodies to F-ACTIN are present in approximately 75% of patients with AIH TYPE 1, approximately 65% of patients with autoimmune Cholagitis, approximately 30% of patients with primary Biliary Cirrhosis, and approximately 2% of healthy controls. High values are closely correlated with AIH TYPE 1. | ||
Setup Schedule / Expected Turnaround Time
Monday, Wednesday, Friday; BS; Reports available: 3 days