Parietal Cell Antibodies, IgG, Serum
PCAB
Test Summary
Evaluating patients suspected of having pernicious anemia or autoimmune-mediated deficiency of vitamin B12 with or without megaloblastic anemia
Aliases
Gastric Parietal Cell
Specimen Collection
Special Instructions
N/A
Preferred Specimen
Centrifuge and aliquot serum into a plastic vial.
Minimum Volume
0.4 mL
Instructions
Centrifuge and aliquot serum into a plastic vial.
Patient Preparation
N/A
Storage
Plastic screw-top vial
Transport Temperature
Refrigerated
Specimen Stability
- Room temperature = N/A
- Refrigerated = 21 days
- Frozen = 21 days
Limitations
- The presence of immune complexes or other immunoglobulin aggregates in the patient specimen may cause an increased nonspecific binding and produce false-positive results in this assay.
- A negative result does not rule out the presence of parietal cell antibodies; the concentration of antibody may be below the detection limit of the assay.
- A positive result only indicates the presence of antibody to H(+)/K(+) ATPase and does not necessarily indicate the presence of autoimmune disease or other diseases.
- The assay performance has not been established for pediatric patients.
- Results of this assay should be used in conjunction with clinical findings and other serological tests.
- The assay performance characteristics have not been established for matrices other than serum.
Other Acceptable Specimens
Serum from a red top tube
Unacceptable Specimens
Gross hemolysis, gross lipemia, heat-treated specimen
Order Code
PCAB
EPIC (Premier) Code
Includes
N/A
CPT Code
83516
Billing Code
671409
CPT Statement
Methodology
Enzyme-Linked Immunosorbent Assay (ELISA)
FDA Status
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
3050 Superior Drive NW
Rochester MN, 55901
Department
Reference Range
See Laboratory Result
Setup Schedule / Expected Turnaround Time
Tuesday, Friday; Report available: 2 - 5 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
Centrifuge and aliquot serum into a plastic vial.
Minimum Volume
0.4 mL
Instructions
Centrifuge and aliquot serum into a plastic vial.
Patient Preparation
N/A
Storage
Plastic screw-top vial
Transport Temperature
Refrigerated
Specimen Stability
- Room temperature = N/A
- Refrigerated = 21 days
- Frozen = 21 days
Limitations
- The presence of immune complexes or other immunoglobulin aggregates in the patient specimen may cause an increased nonspecific binding and produce false-positive results in this assay.
- A negative result does not rule out the presence of parietal cell antibodies; the concentration of antibody may be below the detection limit of the assay.
- A positive result only indicates the presence of antibody to H(+)/K(+) ATPase and does not necessarily indicate the presence of autoimmune disease or other diseases.
- The assay performance has not been established for pediatric patients.
- Results of this assay should be used in conjunction with clinical findings and other serological tests.
- The assay performance characteristics have not been established for matrices other than serum.
Other Acceptable Specimens
Serum from a red top tube
Unacceptable Specimens
Gross hemolysis, gross lipemia, heat-treated specimen
Result Information
Methodology
Enzyme-Linked Immunosorbent Assay (ELISA)
Testing Laboratory
Reference Range
See Laboratory Result
Setup Schedule / Expected Turnaround Time
Tuesday, Friday; Report available: 2 - 5 days