Leukotriene E4, 24 Hour, Urine
TLTE4
Test Summary
Aiding in the evaluation of patients at-risk for mast cell activation syndrome (eg, systemic mastocytosis, IgE-mediated allergies, or aspirin-exacerbated respiratory disease) using 24-hour urine collections
Aliases
- LTE4
- Cysteinyl Leukotriene
Specimen Collection
Special Instructions
Specimen volume (in milliliters) and duration are required
Preferred Specimen
5 mL aliquot of 24 hour urine
Minimum Volume
2 mL
Instructions
Start collection within a few hours of symptom onset and collect urine for 24 hours.
Patient Preparation
Patients taking 5-lipoxygenase inhibitor zileuton (Zyflo) may have decreased concentrations of leukotriene E4 (LTE4). If medically feasible, the patient should not take zileuton for 48 hours before specimen collection.
Storage
Plastic screw-cap vial
Transport Temperature
Frozen
Specimen Stability
Room Temperature: 24 hours
Refrigerated: 7 days
Frozen: 28 days
Limitations
Mast cell (MC) activation disorder is a heterogenous disease, and the absence of elevated LTE4 does not exclude the diagnosis of MC disease. Increased excretion of LTE4 is nonspecific and should not be used alone to make a clinical diagnosis of a MC activation disease.
Other Acceptable Specimens
N/A
Unacceptable Specimens
N/A
Order Code
TLTE4
EPIC (Premier) Code
Includes
Leukotriene E4
Creatinine
CPT Code
82542
Billing Code
655017
CPT Statement
Methodology
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS), Enzymatic Colorimetric Assay
FDA Status
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
3050 Superior Drive NW
Rochester MN, 55901
Department
Reference Range
LEUKOTRIENE E4: < or =104 pg/mg creatinin
CREATININE: <18 years: Not established
Normal values mg per 24 hours:
Males: 930-2955 mg/24 hours
Females: 603-1783 mg/24 hours
Setup Schedule / Expected Turnaround Time
Monday, Tuesday, Thursday; Report available: 2 - 9 days
Specimen Collection
Special Instructions
Specimen volume (in milliliters) and duration are required
Preferred Specimen
5 mL aliquot of 24 hour urine
Minimum Volume
2 mL
Instructions
Start collection within a few hours of symptom onset and collect urine for 24 hours.
Patient Preparation
Patients taking 5-lipoxygenase inhibitor zileuton (Zyflo) may have decreased concentrations of leukotriene E4 (LTE4). If medically feasible, the patient should not take zileuton for 48 hours before specimen collection.
Storage
Plastic screw-cap vial
Transport Temperature
Frozen
Specimen Stability
Room Temperature: 24 hours
Refrigerated: 7 days
Frozen: 28 days
Limitations
Mast cell (MC) activation disorder is a heterogenous disease, and the absence of elevated LTE4 does not exclude the diagnosis of MC disease. Increased excretion of LTE4 is nonspecific and should not be used alone to make a clinical diagnosis of a MC activation disease.
Other Acceptable Specimens
N/A
Unacceptable Specimens
N/A
Result Information
Methodology
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS), Enzymatic Colorimetric Assay
Testing Laboratory
Reference Range
LEUKOTRIENE E4: < or =104 pg/mg creatinin
CREATININE: <18 years: Not established
Normal values mg per 24 hours:
Males: 930-2955 mg/24 hours
Females: 603-1783 mg/24 hours
Setup Schedule / Expected Turnaround Time
Monday, Tuesday, Thursday; Report available: 2 - 9 days