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Ehrlichia chaffeensis Antibodies (IgG, IgM) with Reflex to Titers

Order Code

ECHRF

Test Summary

This test is for the detection of IgG and IgM antibodies against Ehrlichia chaffeensis to aid in the diagnosis of Ehrlichiosis (human monocytic ehrlichiosis). Ehrlichiosis is a tickborne disease caused by transmission of the bacteria via the bite of an infected tick. It is most common in the eastern to southeastern and south-central United States.

Testing for E. chaffeensis is based on a clinical evaluation and risk of tick exposure with consideration to the geographic region. Symptoms may be nonspecific, including headache, fever/chills, malaise, myalgia, gastrointestinal symptoms, and rash. Infection can have similarities with other tickborne illnesses with overlapping vectors, geographic endemicity, and similar clinical signs and symptoms, including Anaplasma spp, Borrelia burgdorferi (Lyme disease) and Babesia microti.

Negative results can occur early in infection. Nucleic acid amplification tests are the preferred method for diagnosis during acute infection. Seroconversion or a four-fold increase between acute and convalescent sera can be used to support a diagnosis. The presence of IgG alone may indicate past infection, and IgM may persist for many months after infection has resolved. Antibody levels may remain elevated for several years after acute illness. Cross-reactivity between related organisms Anaplasma and Ehrlichia spp, can occur. Therefore, interpretation of serologic results is done in the context of pertinent clinical picture, including timing from symptom onset.                

Aliases

N/A

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 mL serum

Minimum Volume

0.2 mL

Instructions

N/A


Patient Preparation

N/A

Storage

Transport tube

Transport Temperature

Room temperature

Specimen Stability

  • Room temperature: 7 days
  • Refrigerated: 14 days
  • Frozen: 30 days

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

N/A

Order Code

ECHRF

EPIC (Premier) Code

LAB6969

Includes

Ehrlichia chaffeensis Antibodies (IgG,IgM).

If E. chaffeensis (IgG) is Detected, then E. chaffeensis (IgG) Titer will be performed at an additional charge (CPT code(s): 86666).

If E. chaffeensis (IgM) is Detected, then E. chaffeensis (IgM) Titer will be performed at an additional charge (CPT code(s): 86666).

CPT Code

86666 (x2)

Billing Code

671359

CPT Statement

Methodology

Immunofluorescence Assay (IFA)

FDA Status

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153

Department

Reference Testing

Reference Range

E. chaffeensis Ab (IgG), Screen Not Detected

E. chaffeensis Ab (IgM), Screen Not Detected

E. chaffeensis Ab (IgG), Titer <1:64 titer

E. chaffeensis Ab (IgM), Titer <1:20 titer               

Setup Schedule / Expected Turnaround Time

Monday, Wednesday - Saturday; Report available: 1 - 3 days

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 mL serum

Minimum Volume

0.2 mL

Instructions

N/A


Patient Preparation

N/A

Storage

Transport tube

Transport Temperature

Room temperature

Specimen Stability

  • Room temperature: 7 days
  • Refrigerated: 14 days
  • Frozen: 30 days

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

N/A

Billing

CPT Code

86666 (x2)

Billing Code

671359

CPT Statement

Result Information

Methodology

Immunofluorescence Assay (IFA)

Testing Laboratory

N/A

Reference Range

E. chaffeensis Ab (IgG), Screen Not Detected

E. chaffeensis Ab (IgM), Screen Not Detected

E. chaffeensis Ab (IgG), Titer <1:64 titer

E. chaffeensis Ab (IgM), Titer <1:20 titer               

Setup Schedule / Expected Turnaround Time

Monday, Wednesday - Saturday; Report available: 1 - 3 days