QuestAssureD™ 25-Hydroxyvitamin D (D2, D3)
Order Code:
QAVITD
Test Summary
Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25-OH D3 - the endogenous form of the vitamin and 25-OH D2 - the analog form used to treat 25-OH Vitamin D3 deficiency.
Aliases
- Vitamin D3
- 25-hydroxy
- D2 Vitamin
- D3 Vitamin
- 25-Hydroxycholecalciferol (25OHD3)
- 25-Hydroxyergocalciferol (25OHD2)
- 25-Hydroxyvitamin D2
- 25-Hydroxyvitamin D3
- 25-OH-D2
- 25-OH-D3
- Vit D
- LC/MS/MS
- Vitamin D2
Specimen Collection
Special Instructions
N/A
Preferred Specimen
0.5 mL serum from a red top tube
Minimum Volume
0.3 mL
Instructions
Collect blood in a serum separator tube (SST), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours. If blood is collected in a red top tube, allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Patient Preparation
Fasting preferred, but not required
Storage
Plastic screw-cap vial
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 21 days
- Refrigerated: 21 days
- Frozen: 21 days
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Gross hemolysis • Grossly lipemic • Heparinized or EDTA plasma • Serum not separated from serum separator tube (SST) gel or clot within 48 hours • Grossly icteric
Order Code
QAVITD
EPIC (Premier) Code
LAB5404
Includes
Vitamin D, 25-OH, Total Vitamin D, 25-OH, D3 Vitamin D, 25-OH, D2
CPT Code
- 82306
Billing Code
- 670004
CPT Statement
Methodology
Chromatography/Mass Spectrometry
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153
14225 Newbrook Drive
Chantilly VA, 20153
Department
Reference Testing
Reference Range
Vitamin D, 25-OH, Total 30-100 ng/mL Vitamin D, 25-OH, D3 Not established Vitamin D, 25-OH, D2 Not established
Setup Schedule / Expected Turnaround Time
Monday - Saturday; Report available: 2 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
0.5 mL serum from a red top tube
Minimum Volume
0.3 mL
Instructions
Collect blood in a serum separator tube (SST), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours. If blood is collected in a red top tube, allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Patient Preparation
Fasting preferred, but not required
Storage
Plastic screw-cap vial
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 21 days
- Refrigerated: 21 days
- Frozen: 21 days
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Gross hemolysis • Grossly lipemic • Heparinized or EDTA plasma • Serum not separated from serum separator tube (SST) gel or clot within 48 hours • Grossly icteric
Result Information
Methodology
Chromatography/Mass Spectrometry
Testing Laboratory
N/A
Reference Range
Vitamin D, 25-OH, Total 30-100 ng/mL Vitamin D, 25-OH, D3 Not established Vitamin D, 25-OH, D2 Not established
Setup Schedule / Expected Turnaround Time
Monday - Saturday; Report available: 2 days