QuestAssureD™ 25-Hydroxyvitamin D (D2, D3)
QAVITD
Test Summary
Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25-OH D3 - the endogenous form of the vitamin and 25-OH D2 - the analog form used to treat 25-OH Vitamin D3 deficiency.
Aliases
- Vitamin D3,25-hydroxy
- D2 Vitamin
- D3 Vitamin
- 25-Hydroxycholecalciferol (25OHD3)
- 25-Hydroxyergocalciferol (25OHD2)
- 25-Hydroxyvitamin D2
- 25-Hydroxyvitamin D3
- 25-OH-D2
- 25-OH-D3
- Vit D
- LC/MS/MS
- Vitamin D2
- 25-hydroxy
Specimen Collection
Special Instructions
N/A
Preferred Specimen
0.5 mL serum red top tube (no-gel)
Minimum Volume
0.3 mL
Instructions
Patient Preparation
Storage
Transport tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 21 days
Refrigerated: 21 days
Frozen: 21 days
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
- Gross hemolysis
- Grossly lipemic
- Heparinized or EDTA plasma
- Serum not separated from serum separator tube (SST) gel or clot within 48 hours
- Grossly icteric
Order Code
QAVITD
EPIC (Premier) Code
LAB5404
Includes
- Vitamin D, 25-OH, Total
- Vitamin D, 25-OH, D3
- Vitamin D, 25-OH, D2
CPT Code
82306
Billing Code
670004
CPT Statement
Methodology
| Chromatography/Mass Spectrometry |
FDA Status
Physician Attestation of Informed Consent
N/A
Testing Laboratory
14225 Newbrook Drive
Chantilly VA, 20153
Department
Reference Range
- Vitamin D, 25-OH, Total 30-100 ng/mL
- Vitamin D, 25-OH D2 Not established
- Vitamin D, 25-OH D3 Not established
Setup Schedule / Expected Turnaround Time
Monday - Saturday; Report available: 2 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
0.5 mL serum red top tube (no-gel)
Minimum Volume
0.3 mL
Instructions
Patient Preparation
Storage
Transport tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 21 days
Refrigerated: 21 days
Frozen: 21 days
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
- Gross hemolysis
- Grossly lipemic
- Heparinized or EDTA plasma
- Serum not separated from serum separator tube (SST) gel or clot within 48 hours
- Grossly icteric
Result Information
Methodology
| Chromatography/Mass Spectrometry |
Testing Laboratory
Reference Range
- Vitamin D, 25-OH, Total 30-100 ng/mL
- Vitamin D, 25-OH D2 Not established
- Vitamin D, 25-OH D3 Not established
Setup Schedule / Expected Turnaround Time
Monday - Saturday; Report available: 2 days