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QuestAssureD™ 25-Hydroxyvitamin D (D2, D3)

Order Code

QAVITD

Test Summary

Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25-OH D3 - the endogenous form of the vitamin and 25-OH D2 - the analog form used to treat 25-OH Vitamin D3 deficiency.

Aliases

  • Vitamin D3,25-hydroxy
  • D2 Vitamin
  • D3 Vitamin
  • 25-Hydroxycholecalciferol (25OHD3)
  • 25-Hydroxyergocalciferol (25OHD2)
  • 25-Hydroxyvitamin D2
  • 25-Hydroxyvitamin D3
  • 25-OH-D2
  • 25-OH-D3
  • Vit D
  • LC/MS/MS
  • Vitamin D2
  • 25-hydroxy

Specimen Collection

Special Instructions

N/A

Preferred Specimen

0.5 mL serum red top tube (no-gel)

Minimum Volume

0.3 mL

Instructions

Collect blood in a serum separator tube (SST), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours. hours.  If blood is collected in a red top tube, allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.

Patient Preparation

Fasting preferred, but not required

Storage

Transport tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 21 days

Refrigerated: 21 days

Frozen: 21 days

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

  • Gross hemolysis
  • Grossly lipemic
  • Heparinized or EDTA plasma
  • Serum not separated from serum separator tube (SST) gel or clot within 48 hours
  • Grossly icteric

Order Code

QAVITD

EPIC (Premier) Code

N/A

Includes

  • Vitamin D, 25-OH, Total
  • Vitamin D, 25-OH, D3
  • Vitamin D, 25-OH, D2

CPT Code

82306

Billing Code

670004

CPT Statement

CPT Statement

Methodology

Chromatography/Mass Spectrometry

FDA Status

This test was developed, and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Quest Diagnostics Nichols Institute
14225 Newbrook Drive
Chantilly VA, 20153

Department

Reference Testing

Reference Range

  • Vitamin D, 25-OH, Total          30-100 ng/mL
  • Vitamin D, 25-OH D2              Not established
  • Vitamin D, 25-OH D3              Not established

Setup Schedule / Expected Turnaround Time

Monday - Saturday; Report available: 2 days 

Specimen Collection

Special Instructions

N/A

Preferred Specimen

0.5 mL serum red top tube (no-gel)

Minimum Volume

0.3 mL

Instructions

Collect blood in a serum separator tube (SST), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours. hours.  If blood is collected in a red top tube, allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.

Patient Preparation

Fasting preferred, but not required

Storage

Transport tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 21 days

Refrigerated: 21 days

Frozen: 21 days

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

  • Gross hemolysis
  • Grossly lipemic
  • Heparinized or EDTA plasma
  • Serum not separated from serum separator tube (SST) gel or clot within 48 hours
  • Grossly icteric

Billing

CPT Code

82306

Billing Code

670004

CPT Statement

CPT Statement

Result Information

Methodology

Chromatography/Mass Spectrometry

Testing Laboratory

N/A

Reference Range

  • Vitamin D, 25-OH, Total          30-100 ng/mL
  • Vitamin D, 25-OH D2              Not established
  • Vitamin D, 25-OH D3              Not established

Setup Schedule / Expected Turnaround Time

Monday - Saturday; Report available: 2 days