Disaccharidases
DISCHA
Test Summary
Lactase, maltase, palatinase and sucrase are mucosal disaccharidases that are present in normal small intestine and fetal colon. A decrease in the enzyme activity of these disaccharidases has been noted in colonic adenomas, adenocarcinomas and malabsorption. Decreased disaccharidase activity is a good indication of mucosal injury with the exception of lactase.
Aliases
N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
5 mg frozen small bowel biopsy collected in a 2 mL cryovial polypropylene screw-cap tube
Minimum Volume
2 mg
Instructions
Place 5 mg small bowel biopsy on the interior surface of a 2 mL polypropylene screw-cap cryovial or small polypropylene collection tube. Freeze within 2 hours of collection at -20° C.
Patient Preparation
N/A
Storage
Frozen
Transport Temperature
Frozen
Specimen Stability
- Room temperature: Unacceptable
- Refrigerated: Unacceptable
- Frozen: 31 days
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Samples frozen later than 2 hours post collection.
Samples received in urine containers.
Samples immersed in a colorless fluid (i.e., water, formalin, saline, etc.).
Samples placed on gauze, filter paper or any other type of support media.
Order Code
DISCHA
EPIC (Premier) Code
LAB6945
Includes
Lactase, Maltase, Palatinase, Sucrase
CPT Code
82657 (x4)
Billing Code
670595
CPT Statement
Methodology
Spectrophotometry (SP)
FDA Status
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
14225 Newbrook Drive
Chantilly VA, 20153
Department
Reference Range
Lactase 15.0-45.5 uM/min/g prot
Sucrase 25.0-69.9 uM/min/g prot
Maltase 100.0-224.4 uM/min/g prot
Palatinase 5.0-26.3 uM/min/g prot
Setup Schedule / Expected Turnaround Time
Sunday -Friday; Report available: 1-3 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
5 mg frozen small bowel biopsy collected in a 2 mL cryovial polypropylene screw-cap tube
Minimum Volume
2 mg
Instructions
Place 5 mg small bowel biopsy on the interior surface of a 2 mL polypropylene screw-cap cryovial or small polypropylene collection tube. Freeze within 2 hours of collection at -20° C.
Patient Preparation
N/A
Storage
Frozen
Transport Temperature
Frozen
Specimen Stability
- Room temperature: Unacceptable
- Refrigerated: Unacceptable
- Frozen: 31 days
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Samples frozen later than 2 hours post collection.
Samples received in urine containers.
Samples immersed in a colorless fluid (i.e., water, formalin, saline, etc.).
Samples placed on gauze, filter paper or any other type of support media.
Result Information
Methodology
Spectrophotometry (SP)
Testing Laboratory
Reference Range
Lactase 15.0-45.5 uM/min/g prot
Sucrase 25.0-69.9 uM/min/g prot
Maltase 100.0-224.4 uM/min/g prot
Palatinase 5.0-26.3 uM/min/g prot
Setup Schedule / Expected Turnaround Time
Sunday -Friday; Report available: 1-3 days