Vitamin D, 25-Hydroxy Total, Screening
Test Summary
Serum 25-OH vitamin D provides a good index of circulating vitamin D activity in patients not suffering from renal disease. Low levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead hypercalcemia.
Aliases
- Vitamin D, 25 Hydroxy Vit D
- 25 OH Vit D
- 25 OH Vitamin D
- Vit D 25 OH
- Vitamin D 25 Hydroxy
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL serum
Minimum Volume
1 mL
Instructions
Barrier tubes (serum separator tubes) are preferred. If non-barrier tubes are utilized, the serum must be removed immediately after centrifuging and placed in a screw-cap transfer tube.
Collection notes: Avoid hemolysis. Invert tubes at least 8 times. Allow to clot in an upright position for 30 minutes. Within 2 hours, centrifuge tubes 10 minutes. Keep tightly stoppered.
Do NOT re-centrifuge tubes. Do NOT freeze the primary collection tube.
Collection notes: Avoid hemolysis. Invert tubes at least 8 times. Allow to clot in an upright position for 30 minutes. Within 2 hours, centrifuge tubes 10 minutes. Keep tightly stoppered.
Do NOT re-centrifuge tubes. Do NOT freeze the primary collection tube.
Patient Preparation
N/A
Storage
Original tube preferred, may be sent in a screw-top transfer tube.
Transport Temperature
Room Temperature or Refrigerated
Specimen Stability
- Room Temperature = 24 hours
- Refrigerated = 5 days
- Frozen = 4 weeks
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Hemolysis, Lipemia, incorrect specimen type, insufficient sample volume, improper storage conditions, exceeds specimen stability guidelines, contaminated sample.
Order Code
VD25
EPIC (Premier) Code
LAB425
Includes
N/A
CPT Code
- 82306
Billing Code
- 300173
CPT Statement
Methodology
Immunoassay (IA)
FDA Status
FDA Approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Department
Chemistry
Reference Range
See Report
Setup Schedule / Expected Turnaround Time
Daily; Report available: Daily
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL serum
Minimum Volume
1 mL
Instructions
Barrier tubes (serum separator tubes) are preferred. If non-barrier tubes are utilized, the serum must be removed immediately after centrifuging and placed in a screw-cap transfer tube.
Collection notes: Avoid hemolysis. Invert tubes at least 8 times. Allow to clot in an upright position for 30 minutes. Within 2 hours, centrifuge tubes 10 minutes. Keep tightly stoppered.
Do NOT re-centrifuge tubes. Do NOT freeze the primary collection tube.
Collection notes: Avoid hemolysis. Invert tubes at least 8 times. Allow to clot in an upright position for 30 minutes. Within 2 hours, centrifuge tubes 10 minutes. Keep tightly stoppered.
Do NOT re-centrifuge tubes. Do NOT freeze the primary collection tube.
Patient Preparation
N/A
Storage
Original tube preferred, may be sent in a screw-top transfer tube.
Transport Temperature
Room Temperature or Refrigerated
Specimen Stability
- Room Temperature = 24 hours
- Refrigerated = 5 days
- Frozen = 4 weeks
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Hemolysis, Lipemia, incorrect specimen type, insufficient sample volume, improper storage conditions, exceeds specimen stability guidelines, contaminated sample.
Result Information
Methodology
Immunoassay (IA)
Testing Laboratory
N/A
Reference Range
See Report
Setup Schedule / Expected Turnaround Time
Daily; Report available: Daily