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Test Directory » Electrophoresis, Immuno And Protein

Electrophoresis, Immuno And Protein

Order Code: SPI

Test Summary

Protein Electrophoresis, with Total Protein and Serum Immunofixation.

Serum protein electrophoresis (SPE) is an analytical technique used to separate serum proteins into six fractions: Albumin, Alpha-1, Alpha-2, Beta-1, Beta-2, and Gamma. Evaluation of increased, decreased, or abnormal protein fraction patterns provides diagnostic information for a variety of conditions, including inflammatory disorders, liver disease, nephrotic syndrome, immune deficiencies, and monoclonal gammopathies (MG). Serum immunofixation electrophoresis (IFE) is performed to identify and characterize monoclonal immunoglobulins (M-proteins) detected by electrophoresis, aiding in the diagnosis and monitoring of plasma cell and lymphoproliferative disorders such as multiple myeloma, Waldenström macroglobulinemia, and monoclonal gammopathy of undetermined significance (MGUS).

Aliases

  • SPEP with IFE
  • Serum Protein Electrophoresis with Immunofixation
  • SPEP/IFE
  • Protein Electrophoresis and Immunofixation
  • Electrophoresis, Immunofixation, and Protein
  • Serum Immunofixation Electrophoresis
  • Immunoelectrophores
  • SPE
  • SPEP
  • IFE

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 mL serum

Minimum Volume

1 mL

Instructions

Avoid hemolysis. Allow to clot in an upright position for 30 minutes. Centrifuge for at least 10 minutes. Keep tightly stoppered.

Patient Preparation

N/A

Storage

Original tube preferred, may be sent in a screw-top transfer tube.

Transport Temperature

Refrigerated

Specimen Stability

  • Room Temperature = 3 days
  • Refrigerated = 10 days
  • Frozen = 2 months

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

Hemolysis, Lipemia, incorrect specimen type, insufficient sample volume, improper storage conditions, exceeds specimen stability guidelines, contaminated sample.

Order Code

SPI

EPIC (Premier) Code

LAB4042

Includes

Immunofixation Serum, Total Protein, Protein Electrophoresis

CPT Code

  • 84155
  • 84165
  • 86334

Billing Code

  • 300366
  • 300536
  • 300362

CPT Statement

CPT Statement

Methodology

See individual tests

FDA Status

FDA Approved

Physician Attestation of Informed Consent

N/A

Testing Laboratory

CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439

Department

Chemistry

Reference Range

See Report

Setup Schedule / Expected Turnaround Time

Monday - Friday; Report available: 2 - 3 days

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 mL serum

Minimum Volume

1 mL

Instructions

Avoid hemolysis. Allow to clot in an upright position for 30 minutes. Centrifuge for at least 10 minutes. Keep tightly stoppered.

Patient Preparation

N/A

Storage

Original tube preferred, may be sent in a screw-top transfer tube.

Transport Temperature

Refrigerated

Specimen Stability

  • Room Temperature = 3 days
  • Refrigerated = 10 days
  • Frozen = 2 months

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

Hemolysis, Lipemia, incorrect specimen type, insufficient sample volume, improper storage conditions, exceeds specimen stability guidelines, contaminated sample.

Billing

CPT Code

  • 84155
  • 84165
  • 86334

Billing Code

  • 300366
  • 300536
  • 300362

CPT Statement

CPT Statement

Result Information

Methodology

See individual tests

Testing Laboratory

N/A

Reference Range

See Report

Setup Schedule / Expected Turnaround Time

Monday - Friday; Report available: 2 - 3 days