Protein Electrophoresis, Serum
Test Summary
Serum protein electrophoresis (SPE) is an analytical technique that provides separation of serum protein into six fractions: Albumin, Alpha-1, Alpha-2, Beta-1, Beta-2, and Gamma. Interpretation of elevation, decreased, or visual change in different fractions can be used as a diagnostic aid for a variety of different disease states and protein abnormalities, including monoclonal gammopathies
Aliases
- Protein, Total and Protein Electrophoresis (Serum), PEP (Serum), PRO ELEC (Serum), SPE, SPEP
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL serum
Minimum Volume
1 mL
Instructions
Barrier tubes (serum separator tubes or plasma separator tubes) are preferred. If non-barrier tubes are utilized, the serum/plasma must be removed immediately after centrifuging and placed in a screw-cap transfer tube.
Collection notes: Avoid hemolysis. Invert tubes at least 8 times. Allow to clot in an upright position for 30 minutes. Within 2 hours, centrifuge tubes 10 minutes. Keep tightly stoppered.
Do NOT re-centrifuge tubes. Do NOT freeze the primary collection tube.
Collection notes: Avoid hemolysis. Invert tubes at least 8 times. Allow to clot in an upright position for 30 minutes. Within 2 hours, centrifuge tubes 10 minutes. Keep tightly stoppered.
Do NOT re-centrifuge tubes. Do NOT freeze the primary collection tube.
Patient Preparation
N/A
Storage
Original tube preferred, may be sent in a screw-top transfer tube.
Transport Temperature
Refrigerated
Specimen Stability
- Room Temperature = 3 days
- Refrigerated = 10 days
- Frozen = 2 months
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Hemolysis, Lipemia, incorrect specimen type, insufficient sample volume, improper storage conditions, exceeds specimen stability guidelines, contaminated sample.
Order Code
SPE
EPIC (Premier) Code
LAB345
Includes
Includes Total Protein
CPT Code
- 84155
- 84165
Billing Code
- 300362
- 300366
CPT Statement
Methodology
electrophoresis
FDA Status
FDA Approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Department
Chemistry
Reference Range
See Report
Setup Schedule / Expected Turnaround Time
Monday - Friday; Report available: 2 - 3 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 mL serum
Minimum Volume
1 mL
Instructions
Barrier tubes (serum separator tubes or plasma separator tubes) are preferred. If non-barrier tubes are utilized, the serum/plasma must be removed immediately after centrifuging and placed in a screw-cap transfer tube.
Collection notes: Avoid hemolysis. Invert tubes at least 8 times. Allow to clot in an upright position for 30 minutes. Within 2 hours, centrifuge tubes 10 minutes. Keep tightly stoppered.
Do NOT re-centrifuge tubes. Do NOT freeze the primary collection tube.
Collection notes: Avoid hemolysis. Invert tubes at least 8 times. Allow to clot in an upright position for 30 minutes. Within 2 hours, centrifuge tubes 10 minutes. Keep tightly stoppered.
Do NOT re-centrifuge tubes. Do NOT freeze the primary collection tube.
Patient Preparation
N/A
Storage
Original tube preferred, may be sent in a screw-top transfer tube.
Transport Temperature
Refrigerated
Specimen Stability
- Room Temperature = 3 days
- Refrigerated = 10 days
- Frozen = 2 months
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Hemolysis, Lipemia, incorrect specimen type, insufficient sample volume, improper storage conditions, exceeds specimen stability guidelines, contaminated sample.
Result Information
Methodology
electrophoresis
Testing Laboratory
N/A
Reference Range
See Report
Setup Schedule / Expected Turnaround Time
Monday - Friday; Report available: 2 - 3 days