T & B For Immunity (Flow Cytometry)
Test Summary
Provides percent and total numbers of T-cell subsets, B cells and Natural Killer (NK) cells.
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
VENOUS PERIPHERAL BLOOD: 5 mLs K2EDTA (lavender top) tube.
Minimum Volume
Minimum 2 - 3 mL
Instructions
Avoid Hemolysis. Invert 10 times.
Patient Preparation
N/A
Storage
K2EDTA (lavender top) tube
Transport Temperature
Room Temperature and transport without delay.
Specimen Stability
- Room Temperature = 24 hours post collection
- Refrigerated = unacceptable
- Frozen = unacceptable
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Rejected for gross hemolysis, clotted. Microcontainers; finger or heel sticks
Order Code
TBIMM
EPIC (Premier) Code
LAB10012
Includes
% T Cells (CD3) and Total Absolute T cells (CD3), % Helper Cells (CD4) and Total Absolute Helper cells (CD4), % Suppressor Cells (CD8) and Total Absolute Suppressor cells (CD8), H/S Ratio, % Natural Killer Cells and Total Absolute Natural Killer cells, % B Cells (CD19) and Total Absolute B cells (CD19).
CPT Code
- 86355
- 86357
- 86360
- 86359
Billing Code
- 300543
- 300544
- 300545
- 300546
CPT Statement
Methodology
Flow Cytometry (FC)
FDA Status
FDA Approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Reference Range
| SEX | AGE | Report Normals | Units | |
| % T Cells (CD3) | N/M/F | 48M | 55 - 82 | % |
| 133Y | 60 - 90 | % | ||
| Total T Cells (CD3) | N/M/F | 6M | 3500 - 5000 | /MM3 |
| 12M | 3400 - 4600 | /MM3 | ||
| 18M | 3200 - 3900 | /MM3 | ||
| 24M | 2800 - 3500 | /MM3 | ||
| 30M | 2300 - 3300 | /MM3 | ||
| 36M | 1900 - 3100 | /MM3 | ||
| 48M | 1000 - 3900 | /MM3 | ||
| 133Y | 720 - 3240 | /MM3 | ||
| % Helper Cells (CD4) | N/M/F | 6M | 50 - 57 | % |
| 12M | 49 - 55 | % | ||
| 18M | 46 - 51 | % | ||
| 24M | 42 - 48 | % | ||
| 30M | 38 - 46 | % | ||
| 36M | 33 - 44 | % | ||
| 48M | 27 - 57 | % | ||
| 133Y | 32 - 61 | % | ||
| Total Helper (CD4) | N/M/F | 6M | 2800 - 3900 | /MM3 |
| 12M | 2600 - 3500 | /MM3 | ||
| 18M | 2300 - 2900 | /MM3 | ||
| 24M | 1900 - 2500 | /MM3 | ||
| 30M | 1500 - 2200 | /MM3 | ||
| 36M | 1200 - 2000 | /MM3 | ||
| 48M | 560 - 2700 | /MM3 | ||
| 133Y | 384 - 2196 | /MM3 | ||
| % Suppressor Cells (CD8) | N/M/F | 36M | 8 - 31 | % |
| 48M | 14 - 34 | % | ||
| 133Y | 15 - 44 | % | ||
| Total Suppressor (CD8) | N/M/F | 36M | 350 - 2500 | /MM3 |
| 48M | 330 - 1400 | /MM3 | ||
| 133Y | 180 - 1584 | /MM3 | ||
| H/S Ratio | N/M/F | 36M | 1.17 - 6.22 | |
| 48M | 0.98 - 3.24 | |||
| 133Y | 0.9 - 3.4 | |||
| % Natural Killer Cells | N/M/F | 48M | Reference Range Not Available | |
| 133Y | Reference Range Not Available | |||
| Total Natural Killer Cell | N/M/F | 48M | Reference Range Not Available | |
| 133Y | Reference Range Not Available | |||
| % B Cells (CD19) | N/M/F | 36M | 11 - 45 | % |
| 48M | 9 - 29 | % | ||
| 133Y | 5 - 15 | % | ||
| Total B Cells | N/M/F | 36M | 430 - 3300 | /MM3 |
| 48M | 200 - 1300 | /MM3 | ||
| 133Y | 60 - 540 | /MM3 | ||
| Comment | N/M/F | ALL |
Setup Schedule / Expected Turnaround Time
Monday - Friday 7:00 am - 6:00 pm. Test not performed on weekends. Report available: 1 - 2 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
VENOUS PERIPHERAL BLOOD: 5 mLs K2EDTA (lavender top) tube.
Minimum Volume
Minimum 2 - 3 mL
Instructions
Avoid Hemolysis. Invert 10 times.
Patient Preparation
N/A
Storage
K2EDTA (lavender top) tube
Transport Temperature
Room Temperature and transport without delay.
Specimen Stability
- Room Temperature = 24 hours post collection
- Refrigerated = unacceptable
- Frozen = unacceptable
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Rejected for gross hemolysis, clotted. Microcontainers; finger or heel sticks
Billing
CPT Code
- 86355
- 86357
- 86360
- 86359
Billing Code
- 300543
- 300544
- 300545
- 300546
CPT Statement
Result Information
Methodology
Flow Cytometry (FC)
Testing Laboratory
N/A
Reference Range
| SEX | AGE | Report Normals | Units | |
| % T Cells (CD3) | N/M/F | 48M | 55 - 82 | % |
| 133Y | 60 - 90 | % | ||
| Total T Cells (CD3) | N/M/F | 6M | 3500 - 5000 | /MM3 |
| 12M | 3400 - 4600 | /MM3 | ||
| 18M | 3200 - 3900 | /MM3 | ||
| 24M | 2800 - 3500 | /MM3 | ||
| 30M | 2300 - 3300 | /MM3 | ||
| 36M | 1900 - 3100 | /MM3 | ||
| 48M | 1000 - 3900 | /MM3 | ||
| 133Y | 720 - 3240 | /MM3 | ||
| % Helper Cells (CD4) | N/M/F | 6M | 50 - 57 | % |
| 12M | 49 - 55 | % | ||
| 18M | 46 - 51 | % | ||
| 24M | 42 - 48 | % | ||
| 30M | 38 - 46 | % | ||
| 36M | 33 - 44 | % | ||
| 48M | 27 - 57 | % | ||
| 133Y | 32 - 61 | % | ||
| Total Helper (CD4) | N/M/F | 6M | 2800 - 3900 | /MM3 |
| 12M | 2600 - 3500 | /MM3 | ||
| 18M | 2300 - 2900 | /MM3 | ||
| 24M | 1900 - 2500 | /MM3 | ||
| 30M | 1500 - 2200 | /MM3 | ||
| 36M | 1200 - 2000 | /MM3 | ||
| 48M | 560 - 2700 | /MM3 | ||
| 133Y | 384 - 2196 | /MM3 | ||
| % Suppressor Cells (CD8) | N/M/F | 36M | 8 - 31 | % |
| 48M | 14 - 34 | % | ||
| 133Y | 15 - 44 | % | ||
| Total Suppressor (CD8) | N/M/F | 36M | 350 - 2500 | /MM3 |
| 48M | 330 - 1400 | /MM3 | ||
| 133Y | 180 - 1584 | /MM3 | ||
| H/S Ratio | N/M/F | 36M | 1.17 - 6.22 | |
| 48M | 0.98 - 3.24 | |||
| 133Y | 0.9 - 3.4 | |||
| % Natural Killer Cells | N/M/F | 48M | Reference Range Not Available | |
| 133Y | Reference Range Not Available | |||
| Total Natural Killer Cell | N/M/F | 48M | Reference Range Not Available | |
| 133Y | Reference Range Not Available | |||
| % B Cells (CD19) | N/M/F | 36M | 11 - 45 | % |
| 48M | 9 - 29 | % | ||
| 133Y | 5 - 15 | % | ||
| Total B Cells | N/M/F | 36M | 430 - 3300 | /MM3 |
| 48M | 200 - 1300 | /MM3 | ||
| 133Y | 60 - 540 | /MM3 | ||
| Comment | N/M/F | ALL |
Setup Schedule / Expected Turnaround Time
Monday - Friday 7:00 am - 6:00 pm. Test not performed on weekends. Report available: 1 - 2 days