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Test Directory Helper/Suppressor Ratio (Flow Cytometry)

Helper/Suppressor Ratio (Flow Cytometry)

Test Summary

Assists in evaluating helper cell immune status in immunodeficiency diseases such as AIDS.

Aliases

Specimen Collection

Special Instructions

Any specimen collected on a Friday or a day prior to a holiday should be called to the Flow Cytometry Lab at 937-208-6687 no later than 4 pm so that transportation can be arranged.

Preferred Specimen

VENOUS PERIPHERAL BLOOD: 5 mLs K2EDTA (lavender top) tube. (minimum 2 - 3 mLs)

Minimum Volume

N/A

Instructions

Avoid hemolysis. Gently invert 10 times.

Patient Preparation

N/A

Storage

K2EDTA (lavender top) tube

Transport Temperature

Room Temperature and transport without delay.

Specimen Stability

Room Temperature = 24 hours post collection
Refrigerated = unacceptable
Frozen = unacceptable

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

Rejected for gross hemolysis, clotted. Microcontainers; finger or heel sticks

Order Code

HSR

EPIC (Premier) Code

LAB10004

Includes

% T Cells (CD3) and Total Absolute T cells (CD3), % Helper Cells (CD4) and Total Absolute Helper cells (CD4), % Suppressor Cells (CD8) and Total Absolute Suppressor cells (CD8), and H/S Ratio.

CPT Code

86359
86360

Billing Code

300546
300545

CPT Statement

Methodology

Flow Cytometry (FC)

FDA Status

FDA Approved

Physician Attestation of Informed Consent

N/A

Testing Laboratory

CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439

Department

Flow Cytometry

Reference Range

% T Cells (CD3)	N/M/F	48M	55 - 82	%
		133Y	60 - 90	%
Total T Cells (CD3)	N/M/F	6M	3500 - 5000	/MM3
		12M	3400 - 4600	/MM3
		18M	3200 - 3900	/MM3
		24M	2800 - 3500	/MM3
		30M	2300 - 3300	/MM3
		36M	1900 - 3100	/MM3
		48M	1000 - 3900	/MM3
		133Y	720 - 3240	/MM3
% Helper Cells (CD4)	N/M/F	6M	50 - 57	%
		12M	49 - 55	%
		18M	46 - 51	%
		24M	42 - 48	%
		30M	38 - 46	%
		36M	33 - 44	%
		48M	27 - 57	%
		133Y	32 - 61	%
Total Helper (CD4)	N/M/F	6M	2800 - 3900	/MM3
		12M	2600 - 3500	/MM3
		18M	2300 - 2900	/MM3
		24M	1900 - 2500	/MM3
		30M	1500 - 2200	/MM3
		36M	1200 - 2000	/MM3
		48M	560 - 2700	/MM3
		133Y	384 - 2196	/MM3
% Suppressor Cells (CD8)	N/M/F	36M	8 - 31	%
		48M	14 - 34	%
		133Y	15 - 44	%
Total Suppressor (CD8)	N/M/F	36M	350 - 2500	/MM3
		48M	330 - 1400	/MM3
		133Y	180 - 1584	/MM3
H/S Ratio	N/M/F	36M	1.17 - 6.22
		48M	0.98 - 3.24
		133Y	0.9 - 3.4

Setup Schedule / Expected Turnaround Time

Monday - Friday 7:00 am - 6:00 pm. Test not performed on weekends. Report available: 1 - 2 days