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Antibody Screen (Indirect Coombs)

Test Summary

This test is used to detect and identify unexpected antibodies directed against red blood cell antigens for use in pretransfusion testing, organ/tissue transplantation, evaluation of transfusion reactions, and to determine the risk for hemolytic disease of the fetus and newborn (HDFN).

Aliases

  • AB Screen
  • Antibody Screen
  • Antibody Screen (Coombs, Indirect)
  • Atypical Antibody Screen
  • Coombs Indirect
  • Indirect Antiglobulin Test
  • Indirect Coombs

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 full large (6 mL) K2EDTA (lavender top or pink top) or two full (3 mL) K2EDTA (lavender top) tube(s).
Pink Top recommended

Minimum Volume

3 mL K2EDTA blood (lavender top or pink top) 1 full 3 mL tube or half filled 6 mL tube. 2 mL whole blood

Instructions

Avoid hemolysis. Gently invert 10 times.

Patient Preparation

N/A

Storage

K2EDTA (lavender top) tube or K2EDTA (pink top) tube---not provided by CompuNet

Transport Temperature

Refrigerated

Specimen Stability

  • Room Temperature = n/a
  • Refrigerated = 3 days
  • Frozen = n/a

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

Rejected for gross hemolysis

Order Code

IATT

EPIC (Premier) Code

LAB625

Includes

When a Antibody Screen is positive, identification of the antibody will be included.

CPT Code

  • 86850

Billing Code

  • 300000

CPT Statement

Methodology

Solid Phase Technology

FDA Status

FDA Approved

Physician Attestation of Informed Consent

N/A

Testing Laboratory

CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439

Reference Range

N/A

Setup Schedule / Expected Turnaround Time

Sunday - Saturday; Report available: 1 day

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 full large (6 mL) K2EDTA (lavender top or pink top) or two full (3 mL) K2EDTA (lavender top) tube(s).
Pink Top recommended

Minimum Volume

3 mL K2EDTA blood (lavender top or pink top) 1 full 3 mL tube or half filled 6 mL tube. 2 mL whole blood

Instructions

Avoid hemolysis. Gently invert 10 times.

Patient Preparation

N/A

Storage

K2EDTA (lavender top) tube or K2EDTA (pink top) tube---not provided by CompuNet

Transport Temperature

Refrigerated

Specimen Stability

  • Room Temperature = n/a
  • Refrigerated = 3 days
  • Frozen = n/a

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

Rejected for gross hemolysis

Billing

CPT Code

  • 86850

Billing Code

  • 300000

CPT Statement

Result Information

Methodology

Solid Phase Technology

Testing Laboratory

N/A

Reference Range

N/A

Setup Schedule / Expected Turnaround Time

Sunday - Saturday; Report available: 1 day