Antibody Screen (Indirect Coombs)
Test Summary
This test is used to detect and identify unexpected antibodies directed against red blood cell antigens for use in pretransfusion testing, organ/tissue transplantation, evaluation of transfusion reactions, and to determine the risk for hemolytic disease of the fetus and newborn (HDFN).
Aliases
- AB Screen
- Antibody Screen
- Antibody Screen (Coombs, Indirect)
- Atypical Antibody Screen
- Coombs Indirect
- Indirect Antiglobulin Test
- Indirect Coombs
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 full large (6 mL) K2EDTA (lavender top or pink top) or two full (3 mL) K2EDTA (lavender top) tube(s).
Pink Top recommended
Pink Top recommended
Minimum Volume
3 mL K2EDTA blood (lavender top or pink top) 1 full 3 mL tube or half filled 6 mL tube. 2 mL whole blood
Instructions
Avoid hemolysis. Gently invert 10 times.
Patient Preparation
N/A
Storage
K2EDTA (lavender top) tube or K2EDTA (pink top) tube---not provided by CompuNet
Transport Temperature
Refrigerated
Specimen Stability
- Room Temperature = n/a
- Refrigerated = 3 days
- Frozen = n/a
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Rejected for gross hemolysis
Order Code
IATT
EPIC (Premier) Code
LAB625
Includes
When a Antibody Screen is positive, identification of the antibody will be included.
CPT Code
- 86850
Billing Code
- 300000
CPT Statement
Methodology
Solid Phase Technology
FDA Status
FDA Approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Reference Range
N/A
Setup Schedule / Expected Turnaround Time
Sunday - Saturday; Report available: 1 day
Specimen Collection
Special Instructions
N/A
Preferred Specimen
1 full large (6 mL) K2EDTA (lavender top or pink top) or two full (3 mL) K2EDTA (lavender top) tube(s).
Pink Top recommended
Pink Top recommended
Minimum Volume
3 mL K2EDTA blood (lavender top or pink top) 1 full 3 mL tube or half filled 6 mL tube. 2 mL whole blood
Instructions
Avoid hemolysis. Gently invert 10 times.
Patient Preparation
N/A
Storage
K2EDTA (lavender top) tube or K2EDTA (pink top) tube---not provided by CompuNet
Transport Temperature
Refrigerated
Specimen Stability
- Room Temperature = n/a
- Refrigerated = 3 days
- Frozen = n/a
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Rejected for gross hemolysis
Result Information
Methodology
Solid Phase Technology
Testing Laboratory
N/A
Reference Range
N/A
Setup Schedule / Expected Turnaround Time
Sunday - Saturday; Report available: 1 day