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Test Directory

Test Directory Direct Antiglobulin Test

Direct Antiglobulin Test

Test Summary

Use to detect in vivo coating of red blood cells (RBCs) with immunoglobulin and complement degradation products on the patient's RBCs. Useful for antibody identification and evaluation of transfusion reactions, hemolytic disease of the fetus and newborn (HDFN), and autoimmune hemolytic anemia associated with disease and/or drugs.

Aliases

Direct Coombs
Coombs
DAT
Direct Antihuman Globulin Test

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 3mL or 6 mL K2EDTA (lavender top or pink top) tube.
Pink Top recommended

Minimum Volume

3 mL K2EDTA blood (lavender top or pink top) (1 full 3 mL tube or half filled 6 mL tube)

Instructions

Avoid hemolysis. Gently invert 10 times.

Patient Preparation

N/A

Storage

K2EDTA (lavender top) tube or K2EDTA (pink top) tube---not provided by CompuNet

Transport Temperature

Refrigerated

Specimen Stability

Room Temperature = n/a
Refrigerated = 3 days
Frozen = n/a

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

Rejected for gross hemolysis

Order Code

DAT

EPIC (Premier) Code

LAB163

Includes

When a DAT is positive, characterization of antibody will be included (IgG and/or complement)

CPT Code

86880

Billing Code

300637

CPT Statement

Methodology

Tube

FDA Status

FDA Approved

Physician Attestation of Informed Consent

N/A

Testing Laboratory

CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439

Department

Blood Transfusion

Reference Range

N/A

Setup Schedule / Expected Turnaround Time

Sunday - Saturday; Report available: 1 day