Flow Cytometry
Order Code:
86486
Test Summary
To aid in the diagnosis, lineage assignment, subclassification, and response to treatment of hematologic malignancies
Aliases
- Acute Leukemia Profile (Flow Cytometry), CLL Lymphoma Profile
Specimen Collection
Special Instructions
Call Flow Cytometry Lab at 937-208-6687 upon collection to arrange for transportation
Preferred Specimen
VENOUS PERIPHERAL BLOOD: 5 mL peripheral blood Sodium Heparin (dark green top) tube and 5 mL peripheral blood K2EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA lavender top tube. (minimum 2 -3 mLs)
BONE MARROW ASPIRATE: 1 mL bone marrow aspirate Sodium Heparin (dark green top) tube, 1 unstained aspirate smear, 1 wright stained aspirate smear, and 5 mL peripheral blood K2EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA lavender top tube. (minimum 1 mL)]
FLUIDS: 2 mL of fluid in Sodium Heparin (dark green top) tube to prevent clotting and keep at room temperature.
FRESH TISSUE: send specimen in media or saline soaked gauze at room temperature with an H&E slide the following day.
CSF: 2 - 4 mL. Keep refrigerated for transport. MUST CALL prior to collection for CSF.
BONE MARROW ASPIRATE: 1 mL bone marrow aspirate Sodium Heparin (dark green top) tube, 1 unstained aspirate smear, 1 wright stained aspirate smear, and 5 mL peripheral blood K2EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA lavender top tube. (minimum 1 mL)]
FLUIDS: 2 mL of fluid in Sodium Heparin (dark green top) tube to prevent clotting and keep at room temperature.
FRESH TISSUE: send specimen in media or saline soaked gauze at room temperature with an H&E slide the following day.
CSF: 2 - 4 mL. Keep refrigerated for transport. MUST CALL prior to collection for CSF.
Minimum Volume
VENOUS PERIPHERAL BLOOD: (minimum 2 -3 mL)
BONE MARROW ASPIRATE: (minimum 1 mL)
FLUIDS: minimum 2 mL
CSF: minimum 2 mL
BONE MARROW ASPIRATE: (minimum 1 mL)
FLUIDS: minimum 2 mL
CSF: minimum 2 mL
Instructions
Avoid hemolysis. Gently invert 10 times.
Tissues & CSF = Call Flow Lab prior to procedure for instructions
Tissues & CSF = Call Flow Lab prior to procedure for instructions
Patient Preparation
N/A
Storage
Sodium Heparin (dark green top) and K2EDTA (lavender top) tube
Tissue: plastic transport tube CSF: CSF collection tube
Tissue: plastic transport tube CSF: CSF collection tube
Transport Temperature
VENOUS PERIPHERAL BLOOD, BONE MARROW ASPIRATES, TISSUE AND FLUIDS: Room Temperature and transport without delay.
CSF: Refrigerate and on ice. Must be in the lab within the hour of collection.
CSF: Refrigerate and on ice. Must be in the lab within the hour of collection.
Specimen Stability
- VENOUS PERIPHERAL BLOOD, BONE MARROW ASPIRATES, AND FLUIDS:
- Room Temperature: 24 hours post collection
- Refrigerated: unacceptable
- Frozen: unacceptable
- CSF:
- Room Temperature = unacceptable
- Refrigerated = 1 to 2 hours post collection and on ice
- Frozen = unacceptable
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
VENOUS PERIPHERAL BLOOD & BONE MARROW ASPIRATE: clotted, hemolyzed or greater than 24 hours old; microcontainers; finger or heel sticks
FLUIDS: greater than 48 hours old
CSF: greater than 4 hours old or room temperature for greater than 1 hour
FLUIDS: greater than 48 hours old
CSF: greater than 4 hours old or room temperature for greater than 1 hour
Order Code
86486
EPIC (Premier) Code
LAB10008
Includes
CD2, CD38, CD45, CD3, CD4, CD5, CD7, CD8, CD19, CD20, CD22, CD23, kappa, lambda, IgG, IgM, IgD, CD11b, CD13, CD14, CD15, CD33, CD117, CD34, HLA-DR, CD10, TdT CD56, CD11c and additional markers as needed.
CPT Code
- 88184 x1(first marker)
- 88185 x# of markers minus the first marker
- 88187 or 88188 or 88189 dependent upon the number of markers interpreted.
Billing Code
- 88184
- 88185
- 88187
- 88188
- 88189
CPT Statement
Methodology
Flow Cytometry (FC)
FDA Status
Lab Developed Test
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Department
Flow Cytometry
Reference Range
N/A
Setup Schedule / Expected Turnaround Time
Monday - Friday 7:00 am - 6:00 pm and on weekends for life threatening Acute Leukemias. Performed at MVH Flow Cytometry department. Reports availabe: 1 - 4 days: critical cases verbal communication with physician.
Specimen Collection
Special Instructions
Call Flow Cytometry Lab at 937-208-6687 upon collection to arrange for transportation
Preferred Specimen
VENOUS PERIPHERAL BLOOD: 5 mL peripheral blood Sodium Heparin (dark green top) tube and 5 mL peripheral blood K2EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA lavender top tube. (minimum 2 -3 mLs)
BONE MARROW ASPIRATE: 1 mL bone marrow aspirate Sodium Heparin (dark green top) tube, 1 unstained aspirate smear, 1 wright stained aspirate smear, and 5 mL peripheral blood K2EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA lavender top tube. (minimum 1 mL)]
FLUIDS: 2 mL of fluid in Sodium Heparin (dark green top) tube to prevent clotting and keep at room temperature.
FRESH TISSUE: send specimen in media or saline soaked gauze at room temperature with an H&E slide the following day.
CSF: 2 - 4 mL. Keep refrigerated for transport. MUST CALL prior to collection for CSF.
BONE MARROW ASPIRATE: 1 mL bone marrow aspirate Sodium Heparin (dark green top) tube, 1 unstained aspirate smear, 1 wright stained aspirate smear, and 5 mL peripheral blood K2EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA lavender top tube. (minimum 1 mL)]
FLUIDS: 2 mL of fluid in Sodium Heparin (dark green top) tube to prevent clotting and keep at room temperature.
FRESH TISSUE: send specimen in media or saline soaked gauze at room temperature with an H&E slide the following day.
CSF: 2 - 4 mL. Keep refrigerated for transport. MUST CALL prior to collection for CSF.
Minimum Volume
VENOUS PERIPHERAL BLOOD: (minimum 2 -3 mL)
BONE MARROW ASPIRATE: (minimum 1 mL)
FLUIDS: minimum 2 mL
CSF: minimum 2 mL
BONE MARROW ASPIRATE: (minimum 1 mL)
FLUIDS: minimum 2 mL
CSF: minimum 2 mL
Instructions
Avoid hemolysis. Gently invert 10 times.
Tissues & CSF = Call Flow Lab prior to procedure for instructions
Tissues & CSF = Call Flow Lab prior to procedure for instructions
Patient Preparation
N/A
Storage
Sodium Heparin (dark green top) and K2EDTA (lavender top) tube
Tissue: plastic transport tube CSF: CSF collection tube
Tissue: plastic transport tube CSF: CSF collection tube
Transport Temperature
VENOUS PERIPHERAL BLOOD, BONE MARROW ASPIRATES, TISSUE AND FLUIDS: Room Temperature and transport without delay.
CSF: Refrigerate and on ice. Must be in the lab within the hour of collection.
CSF: Refrigerate and on ice. Must be in the lab within the hour of collection.
Specimen Stability
- VENOUS PERIPHERAL BLOOD, BONE MARROW ASPIRATES, AND FLUIDS:
- Room Temperature: 24 hours post collection
- Refrigerated: unacceptable
- Frozen: unacceptable
- CSF:
- Room Temperature = unacceptable
- Refrigerated = 1 to 2 hours post collection and on ice
- Frozen = unacceptable
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
VENOUS PERIPHERAL BLOOD & BONE MARROW ASPIRATE: clotted, hemolyzed or greater than 24 hours old; microcontainers; finger or heel sticks
FLUIDS: greater than 48 hours old
CSF: greater than 4 hours old or room temperature for greater than 1 hour
FLUIDS: greater than 48 hours old
CSF: greater than 4 hours old or room temperature for greater than 1 hour
Billing
CPT Code
- 88184 x1(first marker)
- 88185 x# of markers minus the first marker
- 88187 or 88188 or 88189 dependent upon the number of markers interpreted.
Billing Code
- 88184
- 88185
- 88187
- 88188
- 88189
CPT Statement
Result Information
Methodology
Flow Cytometry (FC)
Testing Laboratory
N/A
Reference Range
N/A
Setup Schedule / Expected Turnaround Time
Monday - Friday 7:00 am - 6:00 pm and on weekends for life threatening Acute Leukemias. Performed at MVH Flow Cytometry department. Reports availabe: 1 - 4 days: critical cases verbal communication with physician.