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Test Directory Estradiol and Estrone

Estradiol and Estrone

Test Summary

This panel measures the concentrations of estrone and estradiol, the 2 most potent estrogens that are biologically active. This panel may help assess estrogen status in children and nonpregnant adults.

Estrogens are endogenous steroids that regulate growth and maintenance of sex organs and secondary sex characteristics in women. Estrogens also have other functions and effects, such as production of liver proteins, arterial vasodilation, reduction of intraocular pressure, influence on mood, and maintenance of bone density in both males and females [1]; lack of estrogens has important consequences in both men and women. Low estrogen levels in normal men receiving a gonadotropin releasing hormone agonist and an aromatase inhibitor is associated with decreased sexual function and increased body fat [2]. Measurements of estrogens may help evaluate sexual maturity, menstrual abnormalities, fertility abnormalities, fetal-placental health in pregnancy, tumors that excrete estrogens, or feminization syndromes in men [3].

Increased levels of estrogens may be observed in normal pregnancy, precocious puberty, hyperthyroidism, liver cirrhosis, and ovarian, testicular, and adrenal tumors. Decreased levels of estrogens may be observed in failing pregnancy, Turner syndrome, hypopituitarism, primary and secondary hypogonadism, menopause, polycystic ovarian disease, and anorexia nervosa [4].

Estrone levels may exhibit diurnal rhythm in postmenopausal women because of the adrenal origin of the estrone precursor (androstenedione). Estradiol levels do not have the same diurnal variation [3].

The results of this test should be interpreted in the context of pertinent clinical and family history and physical examination findings.

References
1. Gruber CJ, et al. N Engl J Med. 2002;346(5):340-352.
2. Finkelstein JS, et al. N Engl J Med. 2013;369(11):1011-1022.
3.Nerenz RD, et al. Reproductive endocrinology and related disorders. In: Rifai N, et al. eds. Tietz Textbook of Laboratory Medicine. 7th ed. Elservier Inc; 2022:9521-9714.
4. Pagana K, et al. Mosby's Diagnostic and Laboratory Test Reference. 15th ed. Elsevier Inc; 2020:346-400.

Aliases

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 mL serum collected in a red-top tube (no gel)

Minimum Volume

0.45 mL

Instructions

Specify age and sex on test request form

Patient Preparation

N/A

Storage

N/A

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

Room temperature: 24 hours
Refrigerated: 7 days
Frozen: 28 days

Limitations

N/A

Other Acceptable Specimens

N/A

Unacceptable Specimens

Gross hemolysis • Serum separator tube (SST) • Grossly icteric

Order Code

ESTEST

EPIC (Premier) Code

LAB3641

Includes

Estradiol, Ultrasensitive, LC/MS and Estrone

CPT Code

82670
82679

Billing Code

670872

CPT Statement

Methodology

Chromatography/Mass Spectrometry

FDA Status

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano CA, 92675

Department

Reference Testing

Reference Range

See individual tests

Setup Schedule / Expected Turnaround Time

See individual assays