Desmoglein Antibodies (1 and 3)
Test Summary
To detect the presence of auto antibody specific to Desmoglein 1 and/or 3 in a patients serum as an aid to diagnose type of pemphigus
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
0.3 mL serum
Minimum Volume
0.1 mL
Instructions
Aseptically collect 5 to 8 mL of blood by approved venipuncture procedures.
Allow the blood to clot at room temperature (20 to 25° C) and separate as soon as possible to limit hemolysis. No anticoagulants, or preservatives should be added. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. These conditions may cause aberrant test results. Do not use heat-inactivated serum as this affects test results.
Allow the blood to clot at room temperature (20 to 25° C) and separate as soon as possible to limit hemolysis. No anticoagulants, or preservatives should be added. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. These conditions may cause aberrant test results. Do not use heat-inactivated serum as this affects test results.
Patient Preparation
N/A
Storage
Plastic screw-cap vial
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
- Room temperature: 4 days
- Refrigerated: 7 days
- Frozen: 28 days
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
High levels of hemolysis • High levels of lipemia • Icterus or microbial growth
Order Code
DESMAB
EPIC (Premier) Code
LAB3256
Includes
N/A
CPT Code
- 83520 (x2)
Billing Code
- 670992
CPT Statement
Methodology
Enzyme Linked Immunosorbent Immunoassay (ELISA)
FDA Status
FDA Approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano CA, 92675
33608 Ortega Highway
San Juan Capistrano CA, 92675
Department
Reference Testing
Reference Range
Desmoglein 1 Antibody
Desmoglein 3 Antibody
| Negative | <14 U/mL |
| Equivocal | 14-20 U/mL |
| Positive | >20 U/mL |
Desmoglein 3 Antibody
| Negative | <9 U/mL |
| Equivocal | 9-20 U/mL |
| Positive | >20 U/mL |
Setup Schedule / Expected Turnaround Time
Wednesday, Friday; Report available: 2 - 7 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
0.3 mL serum
Minimum Volume
0.1 mL
Instructions
Aseptically collect 5 to 8 mL of blood by approved venipuncture procedures.
Allow the blood to clot at room temperature (20 to 25° C) and separate as soon as possible to limit hemolysis. No anticoagulants, or preservatives should be added. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. These conditions may cause aberrant test results. Do not use heat-inactivated serum as this affects test results.
Allow the blood to clot at room temperature (20 to 25° C) and separate as soon as possible to limit hemolysis. No anticoagulants, or preservatives should be added. Avoid using sera exhibiting a high level of lipemia, hemolysis, icterus or microbial growth. These conditions may cause aberrant test results. Do not use heat-inactivated serum as this affects test results.
Patient Preparation
N/A
Storage
Plastic screw-cap vial
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
- Room temperature: 4 days
- Refrigerated: 7 days
- Frozen: 28 days
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
High levels of hemolysis • High levels of lipemia • Icterus or microbial growth
Result Information
Methodology
Enzyme Linked Immunosorbent Immunoassay (ELISA)
Testing Laboratory
N/A
Reference Range
Desmoglein 1 Antibody
Desmoglein 3 Antibody
| Negative | <14 U/mL |
| Equivocal | 14-20 U/mL |
| Positive | >20 U/mL |
Desmoglein 3 Antibody
| Negative | <9 U/mL |
| Equivocal | 9-20 U/mL |
| Positive | >20 U/mL |
Setup Schedule / Expected Turnaround Time
Wednesday, Friday; Report available: 2 - 7 days