Acute Leukemia Profile (Flow Cytometry)
Order Code:
71152
Test Summary
To aid in the diagnosis, lineage assignment, subclassification, and response to treatment of hematologic malignancies.
Aliases
- Flow Cytometry
Specimen Collection
Special Instructions
Call Flow Cytometry Lab at 937-208-6687 upon collection to arrange for transportation
Preferred Specimen
VENOUS PERIPHERAL BLOOD: 5 mL peripheral blood Sodium Heparin (dark green top) tube and 5 mL peripheral blood K2EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the EDTA lavender top tube. (minimum 2 -3 mLs)
BONE MARROW ASPIRATE: 1 mL bone marrow aspirate Sodium Heparin (dark green top) tube, 1 unstained aspirate smear, 1 wright stained aspirate smear, and 5 mL peripheral blood EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA lavender top tube. (minimum 1 mL)
FLUIDS: 2 mL of fluid in Sodium Heparin (dark green top) tube to prevent clotting and keep at room temperature.
CSF: 2 - 4 mL. Keep refrigerated for transport. MUST CALL prior to collection for CSF.
BONE MARROW ASPIRATE: 1 mL bone marrow aspirate Sodium Heparin (dark green top) tube, 1 unstained aspirate smear, 1 wright stained aspirate smear, and 5 mL peripheral blood EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA lavender top tube. (minimum 1 mL)
FLUIDS: 2 mL of fluid in Sodium Heparin (dark green top) tube to prevent clotting and keep at room temperature.
CSF: 2 - 4 mL. Keep refrigerated for transport. MUST CALL prior to collection for CSF.
Minimum Volume
VENOUS PERIPHERAL BLOOD: (minimum 2 -3 mLs)
BONE MARROW ASPIRATE: (minimum 1 mL)
FLUIDS: minimum 2 mL
CSF: minimum 2 mL
BONE MARROW ASPIRATE: (minimum 1 mL)
FLUIDS: minimum 2 mL
CSF: minimum 2 mL
Instructions
Avoid hemolysis. Gently invert 10 times.
CSF = Call Flow Cytometry Lab at 937-208-6687 prior to procedure for instructions
CSF = Call Flow Cytometry Lab at 937-208-6687 prior to procedure for instructions
Patient Preparation
N/A
Storage
Sodium Heparin (dark green top) and K2EDTA (lavender top) tube
CSF: Refrigerate and MUST be in the lab within the hour of collection.
CSF: Refrigerate and MUST be in the lab within the hour of collection.
Transport Temperature
VENOUS PERIPHERAL BLOOD, BONE MARROW ASPIRATES, AND FLUIDS: Room Temperature and transport without delay.
CSF: Refrigerate and on ice. Must be in the lab within the hour of collection.
CSF: Refrigerate and on ice. Must be in the lab within the hour of collection.
Specimen Stability
- VENOUS PERIPHERAL BLOOD, BONE MARROW ASPIRATES, AND FLUIDS:
- Room Temperature: 24 hours post collection
- Refrigerated: unacceptable
- Frozen: unacceptable
- CSF:
- Room Temperature = unacceptable
- Refrigerated = 1 to 2 hours post collection and on ice
- Frozen = unacceptable
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
VENOUS PERIPHERAL BLOOD & BONE MARROW ASPIRATE: clotted, hemolyzed or greater than 24 hours old; microcontainers; finger or heel sticks
FLUIDS: greater than 48 hours old
CSF: greater than 4 hours old or room temperature for greater than 1 hour
FLUIDS: greater than 48 hours old
CSF: greater than 4 hours old or room temperature for greater than 1 hour
Order Code
71152
EPIC (Premier) Code
LAB10008
Includes
CD45, CD3, CD4, CD5, CD7, CD8, CD19, CD20, kappa, lambda, CD11b, CD13, CD14, CD15, CD33, CD117, CD34, HLA-DR, CD10, TdT and additional markers as needed.
CPT Code
- 88184 x1(first marker)
- 88185 x# of markers minus the first marker
- 88187 or 88188 or 88189 dependent upon the number of markers interpreted.
Billing Code
- 88184
- 88185
- 88187
- 88188
- 88189
CPT Statement
Methodology
Flow Cytometry (FC)
FDA Status
Lab Developed Test
Physician Attestation of Informed Consent
N/A
Testing Laboratory
CompuNet Clinical Laboratories, LLC
2308 Sandridge Drive
Moraine OH, 45439
2308 Sandridge Drive
Moraine OH, 45439
Department
Flow Cytometry
Reference Range
N/A
Setup Schedule / Expected Turnaround Time
Monday - Friday 7:00 am - 6:00 pm and on weekends for life threatening Acute Leukemias. Performed at MVH Flow Cytometry. Reports availabe: 1 - 4 days: critical cases verbal communication with physician.
Specimen Collection
Special Instructions
Call Flow Cytometry Lab at 937-208-6687 upon collection to arrange for transportation
Preferred Specimen
VENOUS PERIPHERAL BLOOD: 5 mL peripheral blood Sodium Heparin (dark green top) tube and 5 mL peripheral blood K2EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the EDTA lavender top tube. (minimum 2 -3 mLs)
BONE MARROW ASPIRATE: 1 mL bone marrow aspirate Sodium Heparin (dark green top) tube, 1 unstained aspirate smear, 1 wright stained aspirate smear, and 5 mL peripheral blood EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA lavender top tube. (minimum 1 mL)
FLUIDS: 2 mL of fluid in Sodium Heparin (dark green top) tube to prevent clotting and keep at room temperature.
CSF: 2 - 4 mL. Keep refrigerated for transport. MUST CALL prior to collection for CSF.
BONE MARROW ASPIRATE: 1 mL bone marrow aspirate Sodium Heparin (dark green top) tube, 1 unstained aspirate smear, 1 wright stained aspirate smear, and 5 mL peripheral blood EDTA (lavender top) tube, keep both at room temperature, results of a current CBC and a peripheral blood smear may be submitted in place of the K2EDTA lavender top tube. (minimum 1 mL)
FLUIDS: 2 mL of fluid in Sodium Heparin (dark green top) tube to prevent clotting and keep at room temperature.
CSF: 2 - 4 mL. Keep refrigerated for transport. MUST CALL prior to collection for CSF.
Minimum Volume
VENOUS PERIPHERAL BLOOD: (minimum 2 -3 mLs)
BONE MARROW ASPIRATE: (minimum 1 mL)
FLUIDS: minimum 2 mL
CSF: minimum 2 mL
BONE MARROW ASPIRATE: (minimum 1 mL)
FLUIDS: minimum 2 mL
CSF: minimum 2 mL
Instructions
Avoid hemolysis. Gently invert 10 times.
CSF = Call Flow Cytometry Lab at 937-208-6687 prior to procedure for instructions
CSF = Call Flow Cytometry Lab at 937-208-6687 prior to procedure for instructions
Patient Preparation
N/A
Storage
Sodium Heparin (dark green top) and K2EDTA (lavender top) tube
CSF: Refrigerate and MUST be in the lab within the hour of collection.
CSF: Refrigerate and MUST be in the lab within the hour of collection.
Transport Temperature
VENOUS PERIPHERAL BLOOD, BONE MARROW ASPIRATES, AND FLUIDS: Room Temperature and transport without delay.
CSF: Refrigerate and on ice. Must be in the lab within the hour of collection.
CSF: Refrigerate and on ice. Must be in the lab within the hour of collection.
Specimen Stability
- VENOUS PERIPHERAL BLOOD, BONE MARROW ASPIRATES, AND FLUIDS:
- Room Temperature: 24 hours post collection
- Refrigerated: unacceptable
- Frozen: unacceptable
- CSF:
- Room Temperature = unacceptable
- Refrigerated = 1 to 2 hours post collection and on ice
- Frozen = unacceptable
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
VENOUS PERIPHERAL BLOOD & BONE MARROW ASPIRATE: clotted, hemolyzed or greater than 24 hours old; microcontainers; finger or heel sticks
FLUIDS: greater than 48 hours old
CSF: greater than 4 hours old or room temperature for greater than 1 hour
FLUIDS: greater than 48 hours old
CSF: greater than 4 hours old or room temperature for greater than 1 hour
Billing
CPT Code
- 88184 x1(first marker)
- 88185 x# of markers minus the first marker
- 88187 or 88188 or 88189 dependent upon the number of markers interpreted.
Billing Code
- 88184
- 88185
- 88187
- 88188
- 88189
CPT Statement
Result Information
Methodology
Flow Cytometry (FC)
Testing Laboratory
N/A
Reference Range
N/A
Setup Schedule / Expected Turnaround Time
Monday - Friday 7:00 am - 6:00 pm and on weekends for life threatening Acute Leukemias. Performed at MVH Flow Cytometry. Reports availabe: 1 - 4 days: critical cases verbal communication with physician.