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Test Directory T3 (Triiodothyronine) Antibody

T3 (Triiodothyronine) Antibody

Test Summary

This test helps identify triiodothyronine (T3) thyroid hormone autoantibodies (THAAbs), which may interfere with T3 immunoassays in individuals with discordant serum T3 levels [1].

THAAbs may present in healthy individuals but are more common in patients with autoimmune thyroid diseases. THAAbs may affect both 1-step and 2-step immunoassays; the interference may be transient or persist for years. T3 THAAbs interfere with T3 measurements by competing with T3 antibodies used in the T3 immunoassays. When the serum T3 level is discordant with clinical manifestations or other test results (eg, elevated T3 levels with normal or elevated thyroid stimulating hormone [TSH] levels), this test may be used to determine whether T3 THAAbs are the cause of discordance [1].

Other methods of screening for interference by T3 THAAbs are polyethylene glycol precipitation and comparison against equilibrium dialysis. Anomalous T3 levels may also be caused by a range of interfering substances, such as biotin, heterophilic antibodies, and antibodies to streptavidin and ruthenium [1].

Other conditions that may result in elevated free thyroxine and/or free T3 levels without suppressed TSH levels include thyroxine replacement therapy, familial dysalbuminemic hyperthyroxinemia, certain drugs (eg, amiodarone and heparin), nonthyroidal illness, neonatal period, TSH-secreting pituitary adenoma, resistance to thyroid hormone, and disorders of thyroid hormone transport or metabolism [2].

The results of this test should be interpreted in the context of pertinent clinical and family history and physical examination findings.

References
1. Favresse J, et al. Endocr Rev. 2018;39(5):830-850.
2. Gurnell M, et al. Clin Endocrinol (Oxf). 2011;74(6):673-678.

Aliases

Specimen Collection

Special Instructions

N/A

Preferred Specimen

1 mL serum

Minimum Volume

0.5 mL

Instructions

N/A

Patient Preparation

N/A

Storage

Transport tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 14 days
Refrigerated: 14 days
Frozen: 28 days

Limitations

Nonthyroidal chronic diseases, nicotinic acid and nutrition may decrease results. Thyrotoxicosis may not always cause abnormal results. Binding protein variations may cause variations in results. Increases may be associated with pregnancy or oral contraceptive use.

Other Acceptable Specimens

N/A

Unacceptable Specimens

Gross hemolysis • Grossly lipemic

Order Code

T3AB

EPIC (Premier) Code

LAB6095

Includes

N/A

CPT Code

83519

Billing Code

700000

CPT Statement

Methodology

Immunoassay (IA)

FDA Status

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano CA, 92675

Reference Range

Negative

Setup Schedule / Expected Turnaround Time

Sunday, Wedensday; Report available: 4 - 8 days