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Test Directory

FISH, APL, PML/RARA, Translocation 15, 17

Test Summary

This test is performed to detect the PML-RARA fusion, t(15;17), by FISH (fluorescence in situ hybridization). This assay is useful for diagnosis and monitoring of acute promyelocytic leukemia.

Aliases

  • N/A

Specimen Collection

Special Instructions

N/A

Preferred Specimen

5 mL whole blood or 3 mL bone marrow collected in a sodium heparin (green-top) tube or EDTA (lavender-top) tube

Minimum Volume

3 mL whole blood • 1 mL bone marrow

Instructions

Bone marrow 1-3 mL or whole blood 3-5 mL collected in a sodium heparin tube

Patient Preparation

N/A

Storage

N/A

Transport Temperature

Room temperature

Specimen Stability

  • Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Limitations

N/A

Other Acceptable Specimens

Whole blood collected in: Sodium heparin (royal blue-top) tube or sodium heparin lead-free (tan-top) tube • Bone marrow collected in: Bone marrow transport tube (available upon request), sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube

Unacceptable Specimens

N/A

Order Code

PMLRAR

EPIC (Premier) Code

LAB3186

Includes

N/A

CPT Code

  • 88271 (x2)
  • 88275

Billing Code

  • 670946
  • 670947

CPT Statement

Methodology

Fluorescence In Situ Hybridization (FISH)

FDA Status

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Physician Attestation of Informed Consent

N/A

Testing Laboratory

Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano CA, 92675

Department

Reference Testing

Reference Range

See Laboratory Report

Setup Schedule / Expected Turnaround Time

Mornings; 7 days a week. Report available 2 - 3 days.

Specimen Collection

Special Instructions

N/A

Preferred Specimen

5 mL whole blood or 3 mL bone marrow collected in a sodium heparin (green-top) tube or EDTA (lavender-top) tube

Minimum Volume

3 mL whole blood • 1 mL bone marrow

Instructions

Bone marrow 1-3 mL or whole blood 3-5 mL collected in a sodium heparin tube

Patient Preparation

N/A

Storage

N/A

Transport Temperature

Room temperature

Specimen Stability

  • Specimen viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Limitations

N/A

Other Acceptable Specimens

Whole blood collected in: Sodium heparin (royal blue-top) tube or sodium heparin lead-free (tan-top) tube • Bone marrow collected in: Bone marrow transport tube (available upon request), sodium heparin (royal blue-top) tube, or sodium heparin lead-free (tan-top) tube

Unacceptable Specimens

N/A

Billing

CPT Code

  • 88271 (x2)
  • 88275

Billing Code

  • 670946
  • 670947

CPT Statement

Result Information

Methodology

Fluorescence In Situ Hybridization (FISH)

Testing Laboratory

N/A

Reference Range

See Laboratory Report

Setup Schedule / Expected Turnaround Time

Mornings; 7 days a week. Report available 2 - 3 days.