Neutrophil Function, Oxidative Burst
Order Code: NEUTOX
Test Summary
This assay measures the capability of neutrophils (PMN) to undergo oxidative metabolism to produce superoxide anion and hydrogen peroxide, as detected by oxidation of dihydrorhodamine (DHR). Patients with chronic granulomatous disease (CGD) are unable to oxidize DHR due to defects in oxidative capacity slowly decreases as the specimen age (hours post collection) increases. It is thus important that the reference range value appropriate for the specimen age be utilized when assessing the reported results.
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
3 mL whole blood collected in a sodium heparin (green-top) tube
--and--
2nd specimen (Control): 3 mL whole blood in sodium heparin (green-top) tube
--and--
2nd specimen (Control): 3 mL whole blood in sodium heparin (green-top) tube
Minimum Volume
2 mL
Instructions
Specimen must arrive at the referral laboratory within 72 hours of collection in order to meet sample stability requirements. Specimens are accepted <72 hours post collection. No other preservatives are acceptable. Specify time of day specimen was collected. Clients may send 4 mL whole blood in sodium heparin from a healthy donor unrelated to the patient, drawn at the same time as the patient sample to run in parallel with the patient's specimen. The outermost label of the tube must be clearly labeled as "Transport Control". If the draw date is not indicated on the specimen, the client must be called and this information obtained.
Clients: Contact the lab prior to ordering for special logistics arrangements.
PSC: Follow Short Stability Protocol.
Test available by prior arrangement only. Special sample collection and transportation arrangements must be made prior to ordering the test. Contact your local Customer Service Department and request to speak to someone in Referral Testing Department for specific instructions.
Submit Monday through Thursday only. Do not draw day before a holiday. Draw the specimens prior to the last FedEx pick up. Make arrangements with FedEx in advance for same day pick up. Collect and label the specimen(s) according to test requirements. Place the specimen(s) and paperwork into the short stability specimen kit. Use one kit per patient. Use the pre-printed FedEx shipping airbill (provided with the kit) for FedEx Express overnight shipment to Quest Diagnostics Nichols Institute in San Juan Capistrano, CA.
Request item #170903
Samples received > 72 hours after collection will be rejected. Date and time of draw are required and must be provided.
Clients: Contact the lab prior to ordering for special logistics arrangements.
PSC: Follow Short Stability Protocol.
Test available by prior arrangement only. Special sample collection and transportation arrangements must be made prior to ordering the test. Contact your local Customer Service Department and request to speak to someone in Referral Testing Department for specific instructions.
Submit Monday through Thursday only. Do not draw day before a holiday. Draw the specimens prior to the last FedEx pick up. Make arrangements with FedEx in advance for same day pick up. Collect and label the specimen(s) according to test requirements. Place the specimen(s) and paperwork into the short stability specimen kit. Use one kit per patient. Use the pre-printed FedEx shipping airbill (provided with the kit) for FedEx Express overnight shipment to Quest Diagnostics Nichols Institute in San Juan Capistrano, CA.
Request item #170903
Samples received > 72 hours after collection will be rejected. Date and time of draw are required and must be provided.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 72 hours
- Refrigerated: Unacceptable
- Frozen: Unacceptable
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Hemolysis • Lipemia • Icterus • Clotted specimens • Refrigerated and frozen specimens
Order Code
NEUTOX
EPIC (Premier) Code
LAB3897
Includes
N/A
CPT Code
- 82657
Billing Code
- 670351
CPT Statement
Methodology
Flow Cytometry (FC)
FDA Status
The test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulation and is used for clinical purposes.
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano CA, 92675
33608 Ortega Highway
San Juan Capistrano CA, 92675
Department
Reference Testing
Reference Range
%Oxidation Pos Neutrophils
| 24 Hours | ≥93% |
| 48 Hours | ≥83% |
| 72 Hours | ≥82% |
Setup Schedule / Expected Turnaround Time
Daily; Report available: 2 - 3 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
3 mL whole blood collected in a sodium heparin (green-top) tube
--and--
2nd specimen (Control): 3 mL whole blood in sodium heparin (green-top) tube
--and--
2nd specimen (Control): 3 mL whole blood in sodium heparin (green-top) tube
Minimum Volume
2 mL
Instructions
Specimen must arrive at the referral laboratory within 72 hours of collection in order to meet sample stability requirements. Specimens are accepted <72 hours post collection. No other preservatives are acceptable. Specify time of day specimen was collected. Clients may send 4 mL whole blood in sodium heparin from a healthy donor unrelated to the patient, drawn at the same time as the patient sample to run in parallel with the patient's specimen. The outermost label of the tube must be clearly labeled as "Transport Control". If the draw date is not indicated on the specimen, the client must be called and this information obtained.
Clients: Contact the lab prior to ordering for special logistics arrangements.
PSC: Follow Short Stability Protocol.
Test available by prior arrangement only. Special sample collection and transportation arrangements must be made prior to ordering the test. Contact your local Customer Service Department and request to speak to someone in Referral Testing Department for specific instructions.
Submit Monday through Thursday only. Do not draw day before a holiday. Draw the specimens prior to the last FedEx pick up. Make arrangements with FedEx in advance for same day pick up. Collect and label the specimen(s) according to test requirements. Place the specimen(s) and paperwork into the short stability specimen kit. Use one kit per patient. Use the pre-printed FedEx shipping airbill (provided with the kit) for FedEx Express overnight shipment to Quest Diagnostics Nichols Institute in San Juan Capistrano, CA.
Request item #170903
Samples received > 72 hours after collection will be rejected. Date and time of draw are required and must be provided.
Clients: Contact the lab prior to ordering for special logistics arrangements.
PSC: Follow Short Stability Protocol.
Test available by prior arrangement only. Special sample collection and transportation arrangements must be made prior to ordering the test. Contact your local Customer Service Department and request to speak to someone in Referral Testing Department for specific instructions.
Submit Monday through Thursday only. Do not draw day before a holiday. Draw the specimens prior to the last FedEx pick up. Make arrangements with FedEx in advance for same day pick up. Collect and label the specimen(s) according to test requirements. Place the specimen(s) and paperwork into the short stability specimen kit. Use one kit per patient. Use the pre-printed FedEx shipping airbill (provided with the kit) for FedEx Express overnight shipment to Quest Diagnostics Nichols Institute in San Juan Capistrano, CA.
Request item #170903
Samples received > 72 hours after collection will be rejected. Date and time of draw are required and must be provided.
Patient Preparation
N/A
Storage
N/A
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 72 hours
- Refrigerated: Unacceptable
- Frozen: Unacceptable
Limitations
N/A
Other Acceptable Specimens
N/A
Unacceptable Specimens
Hemolysis • Lipemia • Icterus • Clotted specimens • Refrigerated and frozen specimens
Result Information
Methodology
Flow Cytometry (FC)
Testing Laboratory
N/A
Reference Range
%Oxidation Pos Neutrophils
| 24 Hours | ≥93% |
| 48 Hours | ≥83% |
| 72 Hours | ≥82% |
Setup Schedule / Expected Turnaround Time
Daily; Report available: 2 - 3 days