HIV-1 RNA, Quantitative Real-Time PCR with Reflex to Coreceptor Tropism, UDS
Order Code: HV1QTR
Test Summary
This test is intended for use in conjunction with clinical presentation and other laboratory markers of disease progress for the clinical management of HIV-1 infected patients. The test can be used to assess patient prognosis by measuring the baseline HIV-1 RNA level or to monitor the effects of antiretroviral therapy by measuring changes in EDTA plasma HIV-1 RNA levels during the course of antiretroviral treatment. This test is not intended for use as a screening test for the presence of HIV-1 in blood or blood products or as a diagnostic test to confirm the presence of HIV-1 infection.
Aliases
- N/A
Specimen Collection
Special Instructions
N/A
Preferred Specimen
3 mL plasma collected in an EDTA (lavender-top) tube
Minimum Volume
2.5 mL
Instructions
Samples submitted in primary tube will be rejected.
Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube.
Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube.
Patient Preparation
N/A
Storage
Plastic screw-cap vial
Transport Temperature
Frozen
Specimen Stability
- Room temperature: Unacceptable
- Refrigerated: 5 days
- Frozen: 30 days
Limitations
N/A
Other Acceptable Specimens
Plasma collected in: PPT potassium EDTA (white-top) tube
Unacceptable Specimens
Samples received in primary collection tube • Gross hemolysis • Lipemia
Order Code
HV1QTR
EPIC (Premier) Code
LAB5863
Includes
If HIV-1 RNA, Quantitative, PCR is ≥1000 copies/mL, then HIV-1 Coreceptor Tropism, Ultradeep Sequencing will be performed at an additional charge (CPT code(s): 87906).
CPT Code
- 87536
Billing Code
- 670076
CPT Statement
Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
FDA Status
FDA Approved
Physician Attestation of Informed Consent
N/A
Testing Laboratory
Quest Diagnostics Nichols Institute
33608 Ortega Highway
San Juan Capistrano CA, 92675
33608 Ortega Highway
San Juan Capistrano CA, 92675
Department
Reference Testing
Reference Range
| HIV-1 RNA, QN,PCR | Not detected | copies/mL | |
| HIV-1 RNA, QN, PCR | Not detected | Log copies/mL | |
Setup Schedule / Expected Turnaround Time
Monday - Saturday; Report available: 2 - 4 days
Specimen Collection
Special Instructions
N/A
Preferred Specimen
3 mL plasma collected in an EDTA (lavender-top) tube
Minimum Volume
2.5 mL
Instructions
Samples submitted in primary tube will be rejected.
Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube.
Freshly drawn specimens (whole blood) may be stored at 2-25°C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube.
Patient Preparation
N/A
Storage
Plastic screw-cap vial
Transport Temperature
Frozen
Specimen Stability
- Room temperature: Unacceptable
- Refrigerated: 5 days
- Frozen: 30 days
Limitations
N/A
Other Acceptable Specimens
Plasma collected in: PPT potassium EDTA (white-top) tube
Unacceptable Specimens
Samples received in primary collection tube • Gross hemolysis • Lipemia
Result Information
Methodology
Real-Time Polymerase Chain Reaction (RT-PCR)
Testing Laboratory
N/A
Reference Range
| HIV-1 RNA, QN,PCR | Not detected | copies/mL | |
| HIV-1 RNA, QN, PCR | Not detected | Log copies/mL | |
Setup Schedule / Expected Turnaround Time
Monday - Saturday; Report available: 2 - 4 days